Basic Information

Faculty

• Faculty of Engineering

Email Address

• Email Address

  numata.keiji.3nkyoto-u.ac.jp

Academic Degree

• 修士（工学）(東京工業大学)
• 博士（工学)(東京工業大学)

Academic Resume (Graduate Schools)

• 東京工業大学, 大学院総合理工学研究科修士課程物質科学創造専攻, 修了
• 東京工業大学, 大学院総合理工学研究科博士課程物質科学創造専攻, 修了

Academic Resume (Undergraduate School/Majors)

• 東京工業大学, 工学部高分子工学科, 卒業

High School

• High School

  東京学芸大学附属高等学校

Research History

• From Apr. 2020, To Present

  Kyoto University, 材料化学専攻, 教授

ID,URL

• J-Global ID

  201601010345623000

Website(s) (URL(s))

• http://pixy.polym.kyoto-u.ac.jp/index.html

researchmap URL

• https://researchmap.jp/keijinumata_biopoly

Research

Research Areas

• Nanotechnology/Materials, Polymer materials

Papers

• Effect of Oligomers Derived from Biodegradable Polyesters on Eco- and Neurotoxicity

  Naoto Yoshinaga; Ayaka Tateishi; Yasuaki Kobayashi; Tomohiro Kubo; Hitoshi Miyakawa; Kotaro Satoh; Keiji Numata

  Biomacromolecules, 21 Apr. 2023

  
• Papain-Catalyzed, Sequence-Dependent Polymerization Yields Polypeptides Containing Periodic Histidine Residues

  Kayo Terada; Taichi Kurita; Joan Gimenez-Dejoz; Hiroyasu Masunaga; Kousuke Tsuchiya; Keiji Numata

  Macromolecules, 23 Aug. 2022

  
• Unusual pKa Values Mediate the Self-Assembly of Spider Dragline Silk Proteins.

  Nur Alia Oktaviani; Ali D Malay; Akimasa Matsugami; Fumiaki Hayashi; Keiji Numata

  Biomacromolecules, 10 Apr. 2023

  
• Using the organelle glue technique to engineer the plant cell metabolome

  Kazuya Ishikawa; Makoto Kobayashi; Miyako Kusano; Keiji Numata; Yutaka Kodama

  Plant Cell Reports, 27 Jan. 2023

  
• Efficient 3-Hydroxybutyrate Production by Quiescent Escherichia coli Microbial Cell Factories is Facilitated by Indole-Induced Proteomic and Metabolomic Changes

  Nicholas M. Thomson; Tomokazu Shirai; Marco Chiapello; Akihiko Kondo; Krishna J. Mukherjee; Easan Sivaniah; Keiji Numata; David K. Summers

  Biotechnology Journal, 01 May 2018, Peer-reviewed

  
• A high-throughput quantitative method to evaluate peroxisome-chloroplast interactions in Arabidopsis thaliana

  Kazusato Oikawa; Keiko Midorikawa; Yutaka Kodama; Keiji Numata

  Frontiers in Plant Science, 20 Oct. 2022

  
• Organellar Glue: A Molecular Tool to Artificially Control Chloroplast–Chloroplast Interactions

  Shintaro Ichikawa; Shota Kato; Yuta Fujii; Kazuya Ishikawa; Keiji Numata; Yutaka Kodama

  ACS Synthetic Biology, 30 Sep. 2022

  
• 1000 spider silkomes: Linking sequences to silk physical properties

  Kazuharu Arakawa; Nobuaki Kono; Ali D. Malay; Ayaka Tateishi; Nao Ifuku; Hiroyasu Masunaga; Ryota Sato; Kousuke Tsuchiya; Rintaro Ohtoshi; Daniel Pedrazzoli; Asaka Shinohara; Yusuke Ito; Hiroyuki Nakamura; Akio Tanikawa; Yuya Suzuki; Takeaki Ichikawa; Shohei Fujita; Masayuki Fujiwara; Masaru Tomita; Sean J. Blamires; Jo-Ann Chuah; Hamish Craig; Choon P. Foong; Gabriele Greco; Juan Guan; Chris Holland; David L. Kaplan; Kumar Sudesh; Biman B. Mandal; Y. Norma-Rashid; Nur A. Oktaviani; Rucsanda C. Preda; Nicola M. Pugno; Rangam Rajkhowa; Xiaoqin Wang; Kenjiro Yazawa; Zhaozhu Zheng; Keiji Numata

  Science Advances, 14 Oct. 2022

  
• Three-dimensional nanoscale analysis of light-dependent organelle changes in Arabidopsis mesophyll cells

  Keiko Midorikawa; Ayaka Tateishi; Kiminori Toyooka; Mayuko Sato; Takuto Imai; Yutaka Kodama; Keiji Numata

  PNAS Nexus, 04 Oct. 2022

  
• Microbial autotrophic biorefineries: Perspectives for biopolymer production

  Pisanee Srisawat; Mieko Higuchi-Takeuchi; Keiji Numata

  POLYMER JOURNAL, Jul. 2022, Peer-reviewed

  
• Computational study of the interaction between natural rubber alpha-terminal groups and L-quebrachitol, one of the major components of natural rubber

  Joan Gimenez-Dejoz; Katsuhiko Tsunoda; Yasuo Fukushima; Keiji Numata

  POLYMER JOURNAL, Feb. 2022, Peer-reviewed

  
• バイオ高分子が守る資源と環境 : 自然界に存在する材料の理解と利用を目指して

  沼田 圭司

  化学 = Chemistry, Jan. 2022, Peer-reviewed

  
• Molecular dynamics study of the internalization of cell-penetrating peptides containing unnatural amino acids across membranes

  Joan Gimenez-Dejoz; Keiji Numata

  NANOSCALE ADVANCES, Jan. 2022, Peer-reviewed

  
• Chemoenzymatic Synthesis of Sialyl Sulfo-Oligosaccharides as Potent Siglec-8 Ligands via Transglycosylation Catalyzed by Keratanase II

  Shiori Yuge; Ayaka Tateishi; Keiji Numata; Masashi Ohmae

  BIOMACROMOLECULES, Jan. 2022, Peer-reviewed

  
• タンパク質モチーフを利用した機能材料の創出

  沼田 圭司; 大前 仁; 宇治 広隆; 土屋 康佑; 及川 和聡; 藤田 聖矢; Foong Choon Pin; Yagi Shamitha Rao; Thagun Chonprakun; 寺田 佳世

  日本化学繊維研究所講演集 Annual report of the Research Institute for Chemical Fibers, Japan, 2022, Peer-reviewed

  
• クモ糸の階層構造と材料設計

  沼田 圭司

  繊維学会誌, Aug. 2021, Peer-reviewed

  
• クモ糸の階層構造を模倣した人工シルクの創出に向けて

  沼田 圭司

  高分子 = High polymers, Japan : polymers, Jul. 2021, Peer-reviewed

  
• クモ糸の階層構造がはじめて再現された! : 人工クモ糸の誕生に向けての新たな一歩

  沼田 圭司

  化学 = Chemistry, Apr. 2021, Peer-reviewed

  
• ペプチドの自己組織化によるナノキャリア創生

  沼田 圭司; 大前 仁; 宇治 広隆; 土屋 康佑; 及川 和聡; 藤田 聖矢; Choon Pin Foong; 寺田 佳世

  日本化学繊維研究所講演集 Annual report of the Research Institute for Chemical Fibers, Japan, 2021, Peer-reviewed

  
• Functional peptide-mediated plastid transformation in tobacco, rice, and kenaf

  Masaki Odahara; Yoko Horii; Jun Itami; Kenta Watanabe; Keiji Numata

  Frontiers in Plant Science, 23 Sep. 2022

  
• Chemical modification and biosynthesis of silk-like polymers

  Takuya Katashima; Ali D. Malay; Keiji Numata

  CURRENT OPINION IN CHEMICAL ENGINEERING, Jun. 2019, Peer-reviewed

  
• Chemoenzymatic Synthesis of Polypeptides for Use as Functional and Structural Materials

  Kousuke Tsuchiya; Keiji Numata

  MACROMOLECULAR BIOSCIENCE, Nov. 2017, Peer-reviewed

  
• Chemoenzymatic synthesis of polypeptides containing the unnatural amino acid 2-aminoisobutyric acid

  Kousuke Tsuchiya; Keiji Numata

  CHEMICAL COMMUNICATIONS, Jul. 2017, Peer-reviewed

  
• Chemical Synthesis of Multiblock Copolypeptides Inspired by Spider Dragline Silk Proteins

  Kousuke Tsuchiya; Keiji Numata

  ACS MACRO LETTERS, Feb. 2017, Peer-reviewed

  
• Papain-Catalyzed Chemoenzymatic Synthesis of Telechelic Polypeptides Using Bis(Leucine Ethyl Ester) Initiator

  Kousuke Tsuchiya; Keiji Numata

  MACROMOLECULAR BIOSCIENCE, Jul. 2016, Peer-reviewed

  
• The Benzyl Ester Group of Amino Acid Monomers Enhances Substrate Affinity and Broadens the Substrate Specificity of the Enzyme Catalyst in Chemoenzymatic Copolymerization

  Jose Manuel Ageitos; Kenjiro Yazawa; Ayaka Tateishi; Kousuke Tsuchiya; Keiji Numata

  BIOMACROMOLECULES, Jan. 2016, Peer-reviewed

  
• Chemoenzymatic modification of silk fibroin with poly(2,6-dimethyl-1,5-phenylene ether) using horseradish peroxidase

  Leo Simmons; Kousuke Tsuchiya; Keiji Numata

  RSC ADVANCES, 2016, Peer-reviewed

  
• Atomic resolution structure of serine protease proteinase K at ambient temperature.

  Masuda Tetsuya; Suzuki Mamoru; Inoue Shigeyuki; Song Changyong; Nakane Takanori; Nango Eriko; Tanaka Rie; Tono Kensuke; Joti Yasumasa; Kameshima Takashi; Hatsui Takaki; Yabashi Makina; Mikami Bunzo; Nureki Osamu; Numata Keiji; Iwata So; Sugahara Michihiro

  Scientific reports, 31 Mar. 2017

  
• Sucrose supplementation suppressed the growth inhibition in polyhydroxyalkanoate-producing plants.

  Takeshi Yoshizumi; Miwa Yamada; Mieko Higuchi-Takeuchi; Ken'ichiro Matsumoto; Seiichi Taguchi; Minami Matsui; Keiji Numata

  Plant biotechnology (Tokyo, Japan), 2017

  
• Use of extension-deformation-based crystallisation of silk fibres to differentiate their functions in nature

  Keiji Numata; Hiroyasu Masunaga; Takaaki Hikima; Sono Sasaki; Kazuhide Sekiyama; Masaki Takata

  Soft Matter, 21 Aug. 2015, Peer-reviewed

  
• Chemoenzymatic Polymerization of L-Serine Ethyl Ester in Aqueous Media without Side-Group Protection

  Takumi Watanabe; Kayo Terada; Shogo Takemura; Hiroyasu Masunaga; Kousuke Tsuchiya; Alexandros Lamprou; Keiji Numata

  ACS POLYMERS AU, Jun. 2022

  
• Engineered Mutants of a Marine Photosynthetic Purple Nonsulfur Bacterium with Increased Volumetric Productivity of Polyhydroxyalkanoate Bioplastics.

  Choon Pin Foong; Mieko Higuchi-Takeuchi; Kenji Ohtawa; Takuya Asai; Hanqin Liu; Yasuyuki Ozeki; Keiji Numata

  ACS synthetic biology, 18 Feb. 2022

  
• A centrifugation-assisted peptide-mediated gene transfer method for high-throughput analyses.

  Mitsuhiro Kimura; Takeshi Yoshizumi; Keiji Numata

  Plant biotechnology (Tokyo, Japan), 2019, Peer-reviewed

  
• A Screening Method for the Isolation of Polyhydroxyalkanoate-Producing Purple Non-sulfur Photosynthetic Bacteria from Natural Seawater

  Mieko Higuchi-Takeuchi; Kumiko Morisaki; Keiji Numata

  FRONTIERS IN MICROBIOLOGY, Sep. 2016, Peer-reviewed

  
• Double-stranded DNA introduction into intact plants using peptide–DNA complexes

  Manoj Lakshmanan; Takeshi Yoshizumi; Kumar Sudesh; Yutaka Kodama; Keiji Numata

  Plant Biotechnology, 2015, Peer-reviewed

  
• Gene introduction into the mitochondria of Arabidopsis thaliana via peptide-based carriers

  Jo-Ann Chuah; Takeshi Yoshizumi; Yutaka Kodama; Keiji Numata

  SCIENTIFIC REPORTS, Jan. 2015, Peer-reviewed

  
• Synthesis of High-Molecular-Weight Polyhydroxyalkanoates by Marine Photosynthetic Purple Bacteria

  Mieko Higuchi-Takeuchi; Kumiko Morisaki; Kiminori Toyooka; Keiji Numata

  PLOS ONE, Aug. 2016, Peer-reviewed

  
• Intracellular Delivery of Proteins via Fusion Peptides in Intact Plants

  Kiaw Kiaw Ng; Yoko Motoda; Satoru Watanabe; Ahmad Sofiman Othman; Takanori Kigawa; Yutaka Kodama; Keiji Numata

  PLOS ONE, Apr. 2016, Peer-reviewed

  
• Relaxation of the Plant Cell Wall Barrier via Zwitterionic Liquid Pretreatment for Micelle‐Complex‐Mediated DNA Delivery to Specific Plant Organelles

  Takaaki Miyamoto; Kousuke Tsuchiya; Kiminori Toyooka; Yumi Goto; Ayaka Tateishi; Keiji Numata

  Angewandte Chemie International Edition, 07 Jun. 2022

  
• Polymer-coated carbon nanotube hybrids with functional peptides for gene delivery into plant mitochondria.

  Simon Sau Yin Law; Geoffrey Liou; Yukiko Nagai; Joan Giménez-Dejoz; Ayaka Tateishi; Kousuke Tsuchiya; Yutaka Kodama; Tsuyohiko Fujigaya; Keiji Numata

  Nature communications, 16 May 2022

  
• A silk composite fiber reinforced by telechelic-type polyalanine and its strengthening mechanism

  Jianming Chen; Kousuke Tsuchiya; Hiroyasu Masunaga; Ali D. Malay; Keiji Numata

  POLYMER CHEMISTRY, Mar. 2022

  
• Rational Designs at the Forefront of Mitochondria-Targeted Gene Delivery: Recent Progress and Future Perspectives

  Naoto Yoshinaga; Keiji Numata

  ACS Biomaterials Science & Engineering, 14 Feb. 2022

  
• Engineered Nanogel Particles Enhance the Photoautotrophic Biosynthesis of Polyhydroxyalkanoate in Marine Photosynthetic Bacteria

  Pisanee Srisawat; Mieko Higuchi-Takeuchi; Ryutaro Honda; Tomokazu Shirai; Akihiko Kondo; Yu Hoshino; Keiji Numata

  ACS Sustainable Chemistry & Engineering, 04 Apr. 2022

  
• Complexity of Spider Dragline Silk

  Ali D. Malay; Hamish C. Craig; Jianming Chen; Nur Alia Oktaviani; Keiji Numata

  Biomacromolecules, 04 Apr. 2022

  
• Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts.

  Chonprakun Thagun; Yoko Horii; Maai Mori; Seiya Fujita; Misato Ohtani; Kousuke Tsuchiya; Yutaka Kodama; Masaki Odahara; Keiji Numata

  ACS nano, 22 Mar. 2022

  
• An oomycete NLP cytolysin forms transient small pores in lipid membranes

  Katja Pirc; Luke A. Clifton; Neval Yilmaz; Andrea Saltalamacchia; Mojca Mally; Tina Snoj; Nada Žnidaršič; Marija Srnko; Jure Borišek; Petteri Parkkila; Isabell Albert; Marjetka Podobnik; Keiji Numata; Thorsten Nürnberger; Tapani Viitala; Jure Derganc; Alessandra Magistrato; Jeremy H. Lakey; Gregor Anderluh

  Science Advances, 11 Mar. 2022

  
• A Synthetic Multidomain Peptide That Drives a Macropinocytosis-Like Mechanism for Cytosolic Transport of Exogenous Proteins into Plants

  Takaaki Miyamoto; Kiminori Toyooka; Jo-Ann Chuah; Masaki Odahara; Mieko Higchi-Takeuchi; Yumi Goto; Yoko Motoda; Takanori Kigawa; Yutaka Kodama; Keiji Numata

  JACS Au, 24 Jan. 2022

  
• Darwin's bark spider shares a spidroin repertoire with Caerostris extrusa but achieves extraordinary silk toughness through gene expression

  Nobuaki Kono; Rintaro Ohtoshi; Ali D. Malay; Masaru Mori; Hiroyasu Masunaga; Yuki Yoshida; Hiroyuki Nakamura; Keiji Numata; Kazuharu Arakawa

  Open Biology, Dec. 2021

  
• Imaging of the Entry Pathway of a Cell-Penetrating Peptide-DNA Complex From the Extracellular Space to Chloroplast Nucleoids Across Multiple Membranes in Arabidopsis Leaves.

  Kazusato Oikawa; Ayaka Tateishi; Masaki Odahara; Yutaka Kodama; Keiji Numata

  Frontiers in plant science, 2021

  
• Plastics to fertilizers: chemical recycling of a bio-based polycarbonate as a fertilizer source

  Takumi Abe; Rikito Takashima; Takehiro Kamiya; Choon Pin Foong; Keiji Numata; Daisuke Aoki; Hideyuki Otsuka

  Green Chemistry, 2021

  
• Direct introduction of neomycin phosphotransferase II protein into apple leaves to confer kanamycin resistance

  Numata Keiji; Horii Yoko; Motoda Yoko; Hirai Narumi; Nishitani Chikako; Watanabe Satoru; Kigawa Takanori; Kodama Yutaka

  Plant Biotechnology, 2016

  
• The balance of crystalline and amorphous regions in the fibroin structure underpins the tensile strength of bagworm silk

  Nobuaki Kono; Hiroyuki Nakamura; Ayaka Tateishi; Keiji Numata; Kazuharu Arakawa

  Zoological Letters, Dec. 2021

  
• Crystallization-induced mechanofluorescence for visualization of polymer crystallization

  Sota Kato; Shigeki Furukawa; Daisuke Aoki; Raita Goseki; Kazusato Oikawa; Kousuke Tsuchiya; Naohiko Shimada; Atsushi Maruyama; Keiji Numata; Hideyuki Otsuka

  Nature Communications, Dec. 2021

  
• Mitochondrial movement during its association with chloroplasts in Arabidopsis thaliana

  Kazusato Oikawa; Takuto Imai; Chonprakun Thagun; Kiminori Toyooka; Takeshi Yoshizumi; Kazuya Ishikawa; Yutaka Kodama; Keiji Numata

  Communications Biology, Dec. 2021

  
• Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk

  Nobuaki Kono; Hiroyuki Nakamura; Masaru Mori; Yuki Yoshida; Rintaro Ohtoshi; Ali D. Malay; Daniel A. Pedrazzoli Moran; Masaru Tomita; Keiji Numata; Kazuharu Arakawa

  Proceedings of the National Academy of Sciences, 03 Aug. 2021

  
• Draft whole-genome sequence of Bacillus paramycoides LB_RP2, a putative polyhydroxyalkanoate-producing bacterium isolated from an Amazonian blackwater river

  Lorena Mota de Castro; Choon Pin Foong; Mieko Higuchi-Takeuchi; Eraldo Ferreira Lopes; Keiji Numata; Suelen Dias da Silva; Luana da Silva Nonato; Adolfo José da Mota; José Odair Pereira

  MICROBIOLOGY, Jul. 2021, Peer-reviewed

  
• Nanoscale Polyion Complex Vesicles for Delivery of Cargo Proteins and Cas9 Ribonucleoprotein Complexes to Plant Cells

  Masaki Odahara; Kenta Watanabe; Riku Kawasaki; Kousuke Tsuchiya; Ayaka Tateishi; Yoko Motoda; Takanori Kigawa; Yutaka Kodama; Keiji Numata

  ACS Applied Nano Materials, 03 Jun. 2021

  
• All-peptide-based polyion complex vesicles: Facile preparation and encapsulation of the protein in active form

  Seiya Fujita; Kousuke Tsuchiya; Keiji Numata

  ACS POLYMERS, Jun. 2021, Peer-reviewed

  
• Visualization of the Necking Initiation and Propagation Processes during Uniaxial Tensile Deformation of Crystalline Polymer Films via the Generation of Fluorescent Radicals

  Sota Kato; Daisuke Aoki; Kazusato Oikawa; Kousuke Tsuchiya; Keiji Numata; Hideyuki Otsuka

  ACS Macro Letters, 18 May 2021

  
• Construction and Piezoelectric Properties of a Single-Peptide Nanotube Composed of Cyclic β-peptides with Helical Peptides on the Side Chains

  Taichi Kurita; Tomoaki Terabayashi; Shunsaku Kimura; Keiji Numata; Hirotaka Uji

  Biomacromolecules, 17 May 2021

  
• Fusion Peptide-Based Biomacromolecule Delivery System for Plant Cells

  Kenta Watanabe; Masaki Odahara; Takaaki Miyamoto; Keiji Numata

  ACS Biomaterials Science & Engineering, 26 Apr. 2021

  
• Synthetic Mitochondria-Targeting Peptides Incorporating α-Aminoisobutyric Acid with a Stable Amphiphilic Helix Conformation in Plant Cells

  Kayo Terada; Joan Gimenez-Dejoz; Taichi Kurita; Kazusato Oikawa; Hirotaka Uji; Kousuke Tsuchiya; Keiji Numata

  ACS Biomaterials Science & Engineering, 12 Apr. 2021

  
• Xanthurenic Acid Is the Main Pigment of Trichonephila clavata Gold Dragline Silk

  Masayuki Fujiwara; Nobuaki Kono; Akiyoshi Hirayama; Ali D. Malay; Hiroyuki Nakamura; Rintaro Ohtoshi; Keiji Numata; Masaru Tomita; Kazuharu Arakawa

  Biomolecules, 12 Apr. 2021

  
• Lipid Membrane Interaction of Peptide/DNA Complexes Designed for Gene Delivery

  Neval Yilmaz; Yutaka Kodama; Keiji Numata

  Langmuir, 09 Feb. 2021

  
• Reconsidering the “glass transition” hypothesis of intrinsically unstructured CAHS proteins in desiccation tolerance of tardigrades

  Kazuharu Arakawa; Keiji Numata

  Molecular Cell, Feb. 2021

  
• Microbial prospection of an Amazonian blackwater lake and whole-genome sequencing of bacteria capable of polyhydroxyalkanoate synthesis

  Lorena M. Castro; Choon Pin Foong; Mieko Higuchi-Takeuchi; Kumiko Morisaki; Eraldo F. Lopes; Keiji Numata; Adolfo J. Mota

  Polymer Journal, 08 Jan. 2021

  
• Special issue: CO2: capture of, utilization of, and degradation into

  Koji Nakano; Yu Hoshino; Keiji Numata; Keiji Tanaka

  POLYMER JOURNAL, Jan. 2021, Peer-reviewed

  
• Effects of mitochondria-selective fluorescent probes on mitochondrial movement in arabidopsis mesophyll cells evaluated by using the quantification

  Kazusato Oikawa; Takuto Imai; Yutaka Kodama; Keiji Numata

  Plant Biotechnology, 2021

  
• Endosome-escaping micelle complexes dually equipped with cell-penetrating and endosome-disrupting peptides for efficient DNA delivery into intact plants

  Takaaki Miyamoto; Kousuke Tsuchiya; Keiji Numata

  Nanoscale, 2021

  
• Aqueous spinning system with a citrate buffer for highly extensible silk fibers

  Jianming Chen; Yoshinori Ohta; Hiroyuki Nakamura; Hiroyasu Masunaga; Keiji Numata

  Polymer Journal, 01 Jan. 2021

  
• Peptide-Based Polyion Complex Vesicles That Deliver Enzymes into Intact Plants To Provide Antibiotic Resistance without Genetic Modification

  Seiya Fujita; Yoko Motoda; Takanori Kigawa; Kousuke Tsuchiya; Keiji Numata

  Biomacromolecules, 14 Dec. 2020

  
• Simultaneous effect of strain rate and humidity on the structure and mechanical behavior of spider silk

  Kenjiro Yazawa; Ali D. Malay; Hiroyasu Masunaga; Y. Norma-Rashid; Keiji Numata

  Communications Materials, Dec. 2020

  
• Viscosity-adjustable grease matrices for serial nanocrystallography

  Michihiro Sugahara; Koji Motomura; Mamoru Suzuki; Tetsuya Masuda; Yasumasa Joti; Keiji Numata; Kensuke Tono; Makina Yabashi; Tetsuya Ishikawa

  Scientific Reports, Dec. 2020

  
• A marine photosynthetic microbial cell factory as a platform for spider silk production

  Choon Pin Foong; Mieko Higuchi-Takeuchi; Ali D. Malay; Nur Alia Oktaviani; Chonprakun Thagun; Keiji Numata

  Communications Biology, Dec. 2020, Peer-reviewed

  
• Peptide-Mediated Gene Transfer into Marine Purple Photosynthetic Bacteria

  Mieko Higuchi-Takeuchi; Takaaki Miyamoto; Choon Pin Foong; Mami Goto; Kumiko Morisaki; Keiji Numata

  International Journal of Molecular Sciences, Nov. 2020

  
• Spider silk self-assembly via modular liquid-liquid phase separation and nanofibrillation

  Ali D. Malay; Takehiro Suzuki; Takuya Katashima; Nobuaki Kono; Kazuharu Arakawa; Keiji Numata

  Science Advances, Nov. 2020

  
• Development of Reactive Oxygen Species-Triggered Degradable Nanoparticles Using Oligoproline-Containing Peptides

  Riku Kawasaki; Kousuke Tsuchiya; Yutaka Kodama; Keiji Numata

  Biomacromolecules, 12 Oct. 2020, Peer-reviewed

  
• Nearly complete 1H, 13C and 15N chemical shift assignment of monomeric form of N-terminal domain of Nephila clavipes major ampullate spidroin 2

  Nur Alia Oktaviani; Ali D. Malay; Akimasa Matsugami; Fumiaki Hayashi; Keiji Numata

  Biomolecular NMR Assignments, 06 Oct. 2020, Peer-reviewed

  
• How to define and study structural proteins as biopolymer materials

  Keiji Numata

  Polymer Journal, 22 Sep. 2020, Peer-reviewed

  
• Dual Peptide-Based Gene Delivery System for the Efficient Transfection of Plant Callus Cells

  Takaaki Miyamoto; Kousuke Tsuchiya; Keiji Numata

  Biomacromolecules, 13 Jul. 2020, Peer-reviewed

  
• High Capacity Functionalized Protein Superabsorbents from an Agricultural Co-Product: A Cradle-to-Cradle Approach

  Antonio Jose Capezza; Yuxiao Cui; Keiji Numata; Malin Lundman; William Roy Newson; Richard Tobias Olsson; Eva Johansson; Mikael Stefan Hedenqvist

  ADVANCED SUSTAINABLE SYSTEMS, Jul. 2020, Peer-reviewed

  
• Raman image-activated cell sorting

  Nao Nitta; Takanori Iino; Akihiro Isozaki; Mai Yamagishi; Yasutaka Kitahama; Shinya Sakuma; Yuta Suzuki; Hiroshi Tezuka; Minoru Oikawa; Fumihito Arai; Takuya Asai; Dinghuan Deng; Hideya Fukuzawa; Misa Hase; Tomohisa Hasunuma; Takeshi Hayakawa; Kei Hiraki; Kotaro Hiramatsu; Yu Hoshino; Mary Inaba; Yuki Inoue; Takuro Ito; Masataka Kajikawa; Hiroshi Karakawa; Yusuke Kasai; Yuichi Kato; Hirofumi Kobayashi; Cheng Lei; Satoshi Matsusaka; Hideharu Mikami; Atsuhiro Nakagawa; Keiji Numata; Tadataka Ota; Takeichiro Sekiya; Kiyotaka Shiba; Yoshitaka Shirasaki; Nobutake Suzuki; Shunji Tanaka; Shunnosuke Ueno; Hiroshi Watarai; Takashi Yamano; Masayuki Yazawa; Yusuke Yonamine; Dino Di Carlo; Yoichiroh Hosokawa; Sotaro Uemura; Takeaki Sugimura; Yasuyuki Ozeki; Keisuke Goda

  NATURE COMMUNICATIONS, Jul. 2020, Peer-reviewed

  
• Artificial Cell-Penetrating Peptide Containing Periodic α-Aminoisobutyric Acid with Long-Term Internalization Efficiency in Human and Plant Cells

  Kayo Terada; Joan Gimenez-Dejoz; Yu Miyagi; Kazusato Oikawa; Kousuke Tsuchiya; Keiji Numata

  ACS Biomaterials Science & Engineering, 08 Jun. 2020, Peer-reviewed

  
• Zwitterionic Polypeptides: Chemoenzymatic Synthesis and Loosening Function for Cellulose Crystals

  Kousuke Tsuchiya; Neval Yilmaz; Takaaki Miyamoto; Hiroyasu Masunaga; Keiji Numata

  Biomacromolecules, 11 May 2020

  
• Efficient callus induction and a temperature condition for flowering and seed setting in kenaf Hibiscus cannabinus.

  Masaki Odahara; Yoko Horii; Mitsuhiro Kimura; Keiji Numata

  Plant biotechnology (Tokyo, Japan), 25 Mar. 2020

  
• Surface Analysis of Native Spider Draglines by FE-SEM and XPS

  Hiromitsu Sogawa; Kyohei Nakano; Ayaka Tateishi; Keisuke Tajima; Keiji Numata

  Frontiers in Bioengineering and Biotechnology, 20 Mar. 2020

  
• Carotenoids in the eyespot apparatus are required for triggering phototaxis in Euglena gracilis

  Shota Kato; Kazunari Ozasa; Mizuo Maeda; Yuri Tanno; Shun Tamaki; Mieko Higuchi-Takeuchi; Keiji Numata; Yutaka Kodama; Mayuko Sato; Kiminori Toyooka; Tomoko Shinomura

  PLANT JOURNAL, Mar. 2020

  
• Method for the facile transformation of marine purple photosynthetic bacteria using chemically competent cells

  Mieko Higuchi‐Takeuchi; Kumiko Morisaki; Keiji Numata

  MicrobiologyOpen, 22 Jan. 2020

  
• Revealing the Architecture of the Cell Wall in Living Plant Cells by Bioimaging and Enzymatic Degradation

  Neval Yilmaz; Yutaka Kodama; Keiji Numata

  Biomacromolecules, 13 Jan. 2020

  
• Facile terminal functionalization of peptides by protease-catalyzed chemoenzymatic polymerization toward synthesis of polymeric architectures consisting of peptides

  Kousuke Tsuchiya; Keiji Numata

  Polymer Chemistry, 2020

  
• Silk/Natural Rubber (NR) and 3,4-Dihydroxyphenylalanine (DOPA)-Modified Silk/NR Composites: Synthesis, Secondary Structure, and Mechanical Properties

  Hiromitsu Sogawa; Korawit Treratanakulwongs; Hiroyasu Masunaga; Keiji Numata

  Molecules, Jan. 2020

  
• Poly(alanine-nylon-alanine) as a bioplastic: chemoenzymatic synthesis, thermal properties and biological degradation effects

  Prashant G. Gudeangadi; Kei Uchida; Ayaka Tateishi; Kayo Terada; Hiroyasu Masunaga; Kousuke Tsuchiya; Hitoshi Miyakawa; Keiji Numata

  Polymer Chemistry, 2020, Peer-reviewed

  
• Computational study on the polymerization reaction of d-aminopeptidase for the synthesis of d-peptides

  Joan Gimenez-Dejoz; Kousuke Tsuchiya; Ayaka Tateishi; Yoko Motoda; Takanori Kigawa; Yasuhisa Asano; Keiji Numata

  RSC Advances, 2020, Peer-reviewed

  
• A covalently crosslinked silk fibroin hydrogel using enzymatic oxidation and chemoenzymatically synthesized copolypeptide crosslinkers consisting of a GPG tripeptide motif and tyrosine: control of gelation and resilience

  Hiromitsu Sogawa; Takuya Katashima; Keiji Numata

  Polymer Chemistry, 2020, Peer-reviewed

  
• Cellular internalization mechanism of novel Raman probes designed for plant cells

  Keiko Midorikawa; Kousuke Tsuchiya; Simon Sau Yin Law; Yu Miyagi; Takuya Asai; Takanori Iino; Yasuyuki Ozeki; Yutaka Kodama; Keiji Numata

  RSC Chemical Biology, 2020, Peer-reviewed

  
• Simultaneous introduction of multiple biomacromolecules into plant cells using a cell-penetrating peptide nanocarrier

  Chonprakun Thagun; Yoko Motoda; Takanori Kigawa; Yutaka Kodama; Keiji Numata

  Nanoscale, 2020, Peer-reviewed

  
• Targeted Gene Delivery into Various Plastids Mediated by Clustered Cell‐Penetrating and Chloroplast‐Targeting Peptides

  Chonprakun Thagun; Jo‐Ann Chuah; Keiji Numata

  Advanced Science, 23 Dec. 2019

  
• Integrating tough Antheraea pernyi silk and strong carbon fibres for impact-critical structural composites

  Kang Yang; Juan Guan; Keiji Numata; Change Wu; Sujun Wu; Zhengzhong Shao; Robert O. Ritchie

  Nature Communications, Dec. 2019

  
• Periodic introduction of aromatic units in polypeptides via chemoenzymatic polymerization to yield specific secondary structures with high thermal stability

  Kousuke Tsuchiya; Naruki Kurokawa; Joan Gimenez-Dejoz; Prashant G. Gudeangadi; Hiroyasu Masunaga; Keiji Numata

  Polymer Journal, 01 Dec. 2019

  
• The bagworm genome reveals a unique fibroin gene that provides high tensile strength

  Nobuaki Kono; Hiroyuki Nakamura; Rintaro Ohtoshi; Masaru Tomita; Keiji Numata; Kazuharu Arakawa

  Communications Biology, Dec. 2019

  
• Vacuum/Compression Infiltration-mediated Permeation Pathway of a Peptide-pDNA Complex as a Non-Viral Carrier for Gene Delivery in Planta

  Keiko Midorikawa; Yutaka Kodama; Keiji Numata

  Scientific Reports, Dec. 2019

  
• Biomaterials Science and Engineering in Japan: Attractive, Multidisciplinary, and Essential Research Field in Japan

  Akira Matsumoto; Keiji Numata

  ACS Biomaterials Science & Engineering, 11 Nov. 2019

  
• Mechanistic Insights into Silk Fibroin’s Adhesive Properties via Chemical Functionalization of Serine Side Chains

  Cooper J. Love; Bogdan A. Serban; Takuya Katashima; Keiji Numata; Monica A. Serban

  ACS Biomaterials Science & Engineering, 11 Nov. 2019

  
• 3,4-Dihydroxyphenylalanine (DOPA)-Containing Silk Fibroin: Its Enzymatic Synthesis and Adhesion Properties

  Hiromitsu Sogawa; Nao Ifuku; Keiji Numata

  ACS Biomaterials Science & Engineering, 11 Nov. 2019

  
• Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides

  Boyang Guo; Maria Gasset; Jun Itami; Kazusato Oikawa; Yoko Motoda; Takanori Kigawa; Keiji Numata

  PLOS ONE, 30 Jul. 2019

  
• Insights into the Stereospecificity in Papain-Mediated Chemoenzymatic Polymerization from Quantum Mechanics/Molecular Mechanics Simulations

  Joan Gimenez-Dejoz; Kousuke Tsuchiya; Keiji Numata

  ACS Chemical Biology, 21 Jun. 2019

  
• Molecular Interactions and Toughening Mechanisms in Silk Fibroin–Epoxy Resin Blend Films

  Kang Yang; Kenjiro Yazawa; Kousuke Tsuchiya; Keiji Numata; Juan Guan

  Biomacromolecules, 10 Jun. 2019

  
• Cell-Penetrating Peptide-Mediated Transformation of Large Plasmid DNA into Escherichia coli

  Md Monirul Islam; Masaki Odahara; Takeshi Yoshizumi; Kazusato Oikawa; Mitsuhiro Kimura; Masayuki Su’etsugu; Keiji Numata

  ACS Synthetic Biology, 17 May 2019

  
• Optimal iron concentrations for growth-associated polyhydroxyalkanoate biosynthesis in the marine photosynthetic purple bacterium Rhodovulum sulfidophilum under photoheterotrophic condition

  Choon Pin Foong; Martin Koller; Mieko Higuchi-Takeuchi; Keiji Numata

  PLOS ONE, 29 Apr. 2019

  
• Role of Skin Layers on Mechanical Properties and Supercontraction of Spider Dragline Silk Fiber

  Kenjiro Yazawa; Ali D. Malay; Hiroyasu Masunaga; Keiji Numata

  Macromolecular Bioscience, 19 Mar. 2019

  
• Block Copolymer/Plasmid DNA Micelles Postmodified with Functional Peptides via Thiol–Maleimide Conjugation for Efficient Gene Delivery into Plants

  Takaaki Miyamoto; Kousuke Tsuchiya; Keiji Numata

  Biomacromolecules, 11 Feb. 2019

  
• Development of regenerated silk films coated with fluorinated polypeptides to achieve high water repellency and biodegradability in seawater

  Kousuke Tsuchiya; Nao Ifuku; Yasuhito Koyama; Keiji Numata

  Polymer Degradation and Stability, Feb. 2019

  
• Ion effects on the conformation and dynamics of repetitive domains of a spider silk protein: implications for solubility and β-sheet formation

  Nur Alia Oktaviani; Akimasa Matsugami; Fumiaki Hayashi; Keiji Numata

  Chemical Communications, 2019

  
• Screening of a Cell-Penetrating Peptide Library in Escherichia coli: Relationship between Cell Penetration Efficiency and Cytotoxicity

  Kazusato Oikawa; Md Monirul Islam; Yoko Horii; Takeshi Yoshizumi; Keiji Numata

  ACS Omega, 31 Dec. 2018

  
• Library screening of cell-penetrating peptide for BY-2 cells, leaves of Arabidopsis, tobacco, tomato, poplar, and rice callus

  Keiji Numata; Yoko Horii; Kazusato Oikawa; Yu Miyagi; Taku Demura; Misato Ohtani

  Scientific Reports, Dec. 2018

  
• Conformation and dynamics of soluble repetitive domain elucidates the initial β-sheet formation of spider silk

  Nur Alia Oktaviani; Akimasa Matsugami; Ali D. Malay; Fumiaki Hayashi; David L. Kaplan; Keiji Numata

  Nature Communications, Dec. 2018

  
• Spider dragline silk composite films doped with linear and telechelic polyalanine: Effect of polyalanine on the structure and mechanical properties

  Kousuke Tsuchiya; Takaoki Ishii; Hiroyasu Masunaga; Keiji Numata

  Scientific Reports, Dec. 2018

  
• Two Birds with One Stone: Spontaneous Size Separation and Growth Inhibition of Femtosecond Laser-Generated Surfactant-Free Metallic Nanoparticles via ex Situ SU-8 Functionalization

  Dongshi Zhang; Wonsuk Choi; Kenjiro Yazawa; Keiji Numata; Ayaka Tateishi; Sung-Hak Cho; Hsiu-Pen Lin; Yaw Kuen Li; Yoshihiro Ito; Koji Sugioka

  ACS Omega, 30 Sep. 2018

  
• Interplay between Silk Fibroin’s Structure and Its Adhesive Properties

  Erik R. Johnston; Yu Miyagi; Jo-Ann Chuah; Keiji Numata; Monica A. Serban

  ACS Biomaterials Science & Engineering, 13 Aug. 2018

  
• Combination of Amorphous Silk Fiber Spinning and Postspinning Crystallization for Tough Regenerated Silk Fibers

  Kenjiro Yazawa; Ali D. Malay; Nao Ifuku; Takaoki Ishii; Hiroyasu Masunaga; Takaaki Hikima; Keiji Numata

  Biomacromolecules, 11 Jun. 2018

  
• Selective Gene Delivery for Integrating Exogenous DNA into Plastid and Mitochondrial Genomes Using Peptide–DNA Complexes

  Takeshi Yoshizumi; Kazusato Oikawa; Jo-Ann Chuah; Yutaka Kodama; Keiji Numata

  Biomacromolecules, 14 May 2018

  
• Stimulus-Responsive Peptide for Effective Delivery and Release of DNA in Plants

  Jo-Ann Chuah; Keiji Numata

  Biomacromolecules, 09 Apr. 2018

  
• Silk Composite with a Fluoropolymer as a Water-Resistant Protein-Based Material

  Keiji Numata

  Polymers, Apr. 2018

  
• Chemoenzymatic synthesis of polypeptides consisting of periodic di- and tri-peptide motifs similar to elastin

  Prashant G. Gudeangadi; Kousuke Tsuchiya; Takamasa Sakai; Keiji Numata

  Polymer Chemistry, 2018

  
• Class I Polyhydroxyalkanoate Synthase from the Purple Photosynthetic Bacterium Rhodovulum sulfidophilum Predominantly Exists as a Functional Dimer in the Absence of a Substrate

  Mieko Higuchi-Takeuchi; Yoko Motoda; Takanori Kigawa; Keiji Numata

  ACS Omega, 31 Aug. 2017

  
• Silk Resin with Hydrated Dual Chemical-Physical Cross-Links Achieves High Strength and Toughness

  Keiji Numata; Nao Ifuku; Hiroyasu Masunaga; Takaaki Hikima; Takamasa Sakai

  Biomacromolecules, 12 Jun. 2017

  
• Liquid Crystalline Granules Align in a Hierarchical Structure To Produce Spider Dragline Microfibrils

  Ting-Yu Lin; Hiroyasu Masunaga; Ryota Sato; Ali D. Malay; Kiminori Toyooka; Takaaki Hikima; Keiji Numata

  Biomacromolecules, 10 Apr. 2017

  
• Hydroxyethyl cellulose matrix applied to serial crystallography

  Michihiro Sugahara; Takanori Nakane; Tetsuya Masuda; Mamoru Suzuki; Shigeyuki Inoue; Changyong Song; Rie Tanaka; Toru Nakatsu; Eiichi Mizohata; Fumiaki Yumoto; Kensuke Tono; Yasumasa Joti; Takashi Kameshima; Takaki Hatsui; Makina Yabashi; Osamu Nureki; Keiji Numata; Eriko Nango; So Iwata

  SCIENTIFIC REPORTS, Apr. 2017, Peer-reviewed

  
• Tensile Reinforcement of Silk Films by the Addition of Telechelic-Type Polyalanine

  Kousuke Tsuchiya; Hiroyasu Masunaga; Keiji Numata

  Biomacromolecules, 13 Mar. 2017

  
• Analysis of repetitive amino acid motifs reveals the essential features of spider dragline silk proteins.

  Ali D Malay; Kazuharu Arakawa; Keiji Numata

  PloS one, 2017

  
• Chemoenzymatic synthesis of a peptide containing nylon monomer units for thermally processable peptide material application

  Kenjiro Yazawa; Joan Gimenez-Dejoz; Hiroyasu Masunaga; Takaaki Hikima; Keiji Numata

  Polymer Chemistry, 2017

  
• Relationships between physical properties and sequence in silkworm silks.

  Ali D Malay; Ryota Sato; Kenjiro Yazawa; Hiroe Watanabe; Nao Ifuku; Hiroyasu Masunaga; Takaaki Hikima; Juan Guan; Biman B Mandal; Siriporn Damrongsakkul; Keiji Numata

  Scientific reports, 09 Jun. 2016

  
• Oil-free hyaluronic acid matrix for serial femtosecond crystallography.

  Michihiro Sugahara; Changyong Song; Mamoru Suzuki; Tetsuya Masuda; Shigeyuki Inoue; Takanori Nakane; Fumiaki Yumoto; Eriko Nango; Rie Tanaka; Kensuke Tono; Yasumasa Joti; Takashi Kameshima; Takaki Hatsui; Makina Yabashi; Osamu Nureki; Keiji Numata; So Iwata

  Scientific reports, 18 Apr. 2016

  
• Co-expression of Two Polyhydroxyalkanoate Synthase Subunits from Synechocystis sp PCC 6803 by Cell-Free Synthesis and Their Specific Activity for Polymerization of 3-Hydroxybutyryl-Coenzyme A

  Keiji Numata; Yoko Motoda; Satoru Watanabe; Takashi Osanai; Takanori Kigawa

  BIOCHEMISTRY, Feb. 2015

  
• Understanding the Limitations in the Biosynthesis of Polyhydroxyalkanoate (PHA) from Lignin Derivatives

  Tomizawa, Satoshi; Chuah, Jo-Ann; Matsumoto, Keiji; Doi, Yoshiharu; Numata, Keiji

  Acs Sustainable Chemistry & Engineering, 2014

  
• Synthesis of Homopolypeptides by Aminolysis Mediated by Proteases Encapsulated in Silica Nanospheres

  Baker, Peter J.; Patwardhan, Siddharth V.; Numata, Keiji

  Macromolecular Bioscience, 2014

  
• Synthesis of Adhesive Peptides Similar to Those Found in Blue Mussel (Mytilus edulis) Using Papain and Tyrosinase

  Numata, Keiji; Baker, Peter James

  Biomacromolecules, 2014

  
• Silk-Pectin Hydrogel with Superior Mechanical Properties, Biodegradability, and Biocompatibility

  Numata, Keiji; Yamazaki, Shoya; Katashima, Takuya; Chuah, Jo-Ann; Naga, Naofumi; Sakai, Takamasa

  Macromolecular Bioscience, 2014

  
• Short One-Pot Chemo-Enzymatic Synthesis of L-Lysine and L-Alanine Diblock Co-Oligopeptides

  Fagerland, Jenny; Finne-Wistrand, Anna; Numata, Keiji

  Biomacromolecules, 2014

  
• Recent Advances in Chemoenzymatic Peptide Syntheses

  Yazawa, Kenjiro; Numata, Keiji

  Molecules, 2014

  
• Phasin Proteins Activate Aeromonas caviae Polyhydroxyalkanoate (PHA) Synthase but Not Ralstonia eutropha PHA Synthase

  Ushimaru, Kazunori; Motoda, Yoko; Numata, Keiji; Tsuge, Takeharu

  Applied and Environmental Microbiology, 2014

  
• Pathway-Level Acceleration of Glycogen Catabolism by a Response Regulator in the Cyanobacterium Synechocystis Species PCC 6803

  Osanai, Takashi; Oikawa, Akira; Numata, Keiji; Kuwahara, Ayuko; Iijima, Hiroko; Doi, Yoshiharu; Saito, Kazuki; Hirai, Masami Yokota

  Plant Physiology, 2014

  
• Modification of beta-oxidation pathway in Ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from soybean oil

  Insomphun, Chayatip; Mifune, Jun; Orita, Izumi; Numata, Keiji; Nakamura, Satoshi; Fukui, Toshiaki

  Journal of Bioscience and Bioengineering, 2014

  
• Local gene silencing in plants via synthetic dsRNA and carrier peptide

  Numata, Keiji; Ohtani, Misato; Yoshizumi, Takeshi; Demura, Taku; Kodama, Yutaka

  Plant Biotechnology Journal, 2014

  
• Biosynthesis of Polyhydroxyalkanoate by a Marine Bacterium Vibrio sp Strain Using Sugars, Plant Oil, and Unsaturated Fatty Acids as Sole Carbon Sources

  Satoshi Tomizawa; Jo-Ann Chuah; Misato Ohtani; Taku Demura; Keiji Numata

  GREEN POLYMER CHEMISTRY: BIOCATALYSIS AND MATERIALS II, 2013

  
• Synthesis of poly- and oligo(hydroxyalkanoate)s by deep-sea bacteria, Colwellia spp., Moritella spp., and Shewanella spp

  Numata, Keiji; Morisaki, Kumiko; Tomizawa, Satoshi; Ohtani, Misato; Demura, Taku; Miyazaki, Masayuki; Nogi, Yuichi; Deguchi, Shigeru; Doi, Yoshiharu

  Polymer Journal, 2013

  
• Rapid and Efficient Gene Delivery into Plant Cells Using Designed Peptide Carriers

  Lakshmanan, Manoj; Kodama, Yutaka; Yoshizumi, Takeshi; Sudesh, Kumar; Numata, Keiji

  Biomacromolecules, 2013

  
• Proteinase K-Catalyzed Synthesis of Linear and Star Oligo(L-phenylalanine) Conjugates

  Ageitos, Jose M.; Baker, Peter J.; Sugahara, Michihiro; Numata, Keiji

  Biomacromolecules, 2013

  
• Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]

  Yamada, Miwa; Takahashi, Shuntaro; Okahata, Yoshio; Doi, Yoshiharu; Numata, Keiji

  Amb Express, 2013

  
• Increased Bioplastic Production with an RNA Polymerase Sigma Factor SigE during Nitrogen Starvation in Synechocystis sp PCC 6803

  Osanai, Takashi; Numata, Keiji; Oikawa, Akira; Kuwahara, Ayuko; Iijima, Hiroko; Doi, Yoshiharu; Tanaka, Kan; Saito, Kazuki; Hirai, Masami Yokota

  DNA Research, 2013

  
• Characterization of Site-Specific Mutations in a Short-Chain-Length/Medium-Chain-Length Polyhydroxyalkanoate Synthase: In Vivo and In Vitro Studies of Enzymatic Activity and Substrate Specificity

  Chuah, Jo-Ann; Tomizaw, Satoshi; Yamad, Miwa; Tsuge, Takeharu; Doi, Yoshiharu; Sudesh, Kumar; Numata, Keiji

  Applied and Environmental Microbiology, 2013

  
• Biosynthesis and characterization of polyhydroxyalkanoate containing 5-hydroxyvalerate units: Effects of 5HV units on biodegradability, cytotoxicity, mechanical and thermal properties

  Chuah, Jo-Ann; Yamada, Miwa; Taguchi, Seiichi; Sudesh, Kumar; Doi, Yoshiharu; Numata, Keiji

  Polymer Degradation and Stability, 2013

  
• Biopolymer-Based Nanoparticles for Drug/Gene Delivery and Tissue Engineering

  Nitta, Sachiko Kaihara; Numata, Keiji

  International Journal of Molecular Sciences, 2013

  
• Biodegradability of nylon 4 film in a marine environment

  Tachibana, Koichiro; Urano, Yuichi; Numata, Keiji

  Polymer Degradation and Stability, 2013

  
• Silk-Based Nanocomplexes with Tumor-Homing Peptides for Tumor-Specific Gene Delivery

  Numata, Keiji; Mieszawska-Czajkowska, Aneta J.; Kvenvold, Laura A.; Kaplan, David L.

  Macromolecular Bioscience, 2012

  
• Chemoenzymatic Synthesis of Poly(L-alanine) in Aqueous Environment

  Baker, Peter James; Numata, Keiji

  Biomacromolecules, 2012

  
• Chemo-enzymatic synthesis of peptide polymers and their application for gene delivery

  Numata, Keiji

  Abstracts of Papers of the American Chemical Society, 2012

  
• Biosynthesis of Polyhydroxyalkanaotes by a Novel Facultatively Anaerobic Vibrio sp under Marine Conditions

  Numata, Keiji; Doi, Yoshiharu

  Marine Biotechnology, 2012

  
• Biocompatible and Biodegradable Dual-Drug Release System Based on Silk Hydrogel Containing Silk Nanoparticles

  Numata, Keiji; Yamazaki, Shoya; Naga, Naofumi

  Biomacromolecules, 2012

  
• Active Intermediates of Polyhydroxyalkanoate Synthase from Aeromonas caviae in Polymerization Reaction

  Numata, Keiji; Motoda, Yoko; Watanabe, Satoru; Tochio, Naoya; Kigawa, Takanori; Doi, Yoshiharu

  Biomacromolecules, 2012

  
• State of Water, Molecular Structure, and Cytotoxicity of Silk Hydrogels

  Numata, Keiji; Katashima, Takuya; Sakai, Takamasa

  Biomacromolecules, 2011

  
• Spider Silk-Based Gene Carriers for Tumor Cell-Specific Delivery

  Numata, Keiji; Reagan, Michaela R.; Goldstein, Robert H.; Rosenblatt, Michael; Kaplan, David L.

  Bioconjugate Chemistry, 2011

  
• Dual biosyntheses of poly[(R)-3-hydroxybutyric acid] and silk protein for the fabrication of biofunctional bioplastic

  Numata, Keiji; Doi, Yoshiharu

  Polymer Journal, 2011

  
• Differences in Cytotoxicity of beta-Sheet Peptides Originated from Silk and Amyloid beta

  Numata, Keiji; Kaplan, David L.

  Macromolecular Bioscience, 2011

  
• AFM Study of Morphology and Mechanical Properties of a Chimeric Spider Silk and Bone Sialoprotein Protein for Bone Regeneration

  Gomes, Silvia; Numata, Keiji; Leonor, Isabel B.; Mano, Joao F.; Reis, Rui L.; Kapan, David L.

  Biomacromolecules, 2011

  
• Surface-patterned electrode bioreactor for electrical stimulation

  Tandon, Nina; Marsano, Anna; Maidhof, Robert; Numata, Keiji; Montouri-Sorrentino, Chrystina; Cannizzaro, Christopher; Voldman, Joel; Vunjak-Novakovic, Gordana

  Lab on a Chip, 2010

  
• Silk-Based Gene Carriers with Cell Membrane Destabilizing Peptides

  Numata, Keiji; Kaplan, David L.

  Biomacromolecules, 2010

  
• Reinforcing Silk Scaffolds with Silk Particles

  Rajkhowa, Rangam; Gil, Eun Seok; Kluge, Jonathan; Numata, Keiji; Wang, Lijing; Wang, Xungai; Kaplan, David L.

  Macromolecular Bioscience, 2010

  
• Quantifying Osteogenic Cell Degradation of Silk Biomaterials

  Sengupta, Sejuti; Park, Sang-Hyug; Seok, Gil Eun; Patel, Atur; Numata, Keiji; Lu, Chia-Li; Kaplan, David L.

  Biomacromolecules, 2010

  
• Mechanisms of Enzymatic Degradation of Amyloid beta Microfibrils Generating Nanofilaments and Nanospheres Related to Cytotoxicity

  Numata, Keiji; Kaplan, David L.

  Biochemistry, 2010

  
• Mechanism of enzymatic degradation of beta-sheet crystals

  Numata, Keiji; Cebe, Peggy; Kaplan, David L.

  Biomaterials, 2010

  
• Gene delivery mediated by recombinant silk proteins containing cationic and cell binding motifs

  Numata, Keiji; Hamasaki, Juliana; Subramanian, Balajikarthick; Kaplan, David L.

  Journal of Controlled Release, 2010

  
• Expression, Cross-Linking, and Characterization of Recombinant Chitin Binding Resilin

  Qin, Guokui; Lapidot, Shaul; Numata, Keiji; Hu, Xiao; Meirovitch, Sigal; Dekel, Mara; Podoler, Itai; Shoseyov, Oded; Kaplan, David L.

  Biomacromolecules, 2009

  
• Bioengineered silk protein-based gene delivery systems

  Numata, Keiji; Subramanian, Balajikarthick; Currie, Heather A.; Kaplan, David L.

  Biomaterials, 2009

  
• Biodegradability of Poly(hydroxyalkanoate) Materials

  Numata, Keiji; Abe, Hideki; Iwata, Tadahisa

  Materials, 2009

  
• Enzymatic processes for biodegradation of poly(hydroxyalkanoate)s crystals

  Numata, Keiji; Abe, Hideki; Doi, Yoshiharu

  Canadian Journal of Chemistry-Revue Canadienne De Chimie, 2008

  
• Enzymatic degradation of monolayer for poly(lactide) revealed by real-time atomic force microscopy: Effects of stereochemical structure, molecular weight, and molecular branches on hydrolysis rates

  Numata, Keiji; Finne-Wistrand, Anna; Albertsson, Ann-Christine; Doi, Yoshiharu; Abe, Hideki

  Biomacromolecules, 2008

  
• Branched poly(lactide) synthesized by enzymatic polymerization: Effects of molecular branches and stereochernistry on enzymatic degradation and alkaline hydrolysis

  Numata, Keiji; Srivastava, Rajiv K.; Finne-Wistrand, Anna; Albertsson, Ann-Christine; Doi, Yoshiharu; Abe, Hideki

  Biomacromolecules, 2007

  
• Adsorption effects of poly (hydroxybutyric acid) depolymerase on chain-folding surface of polyester single crystals revealed by mutant enzyme and frictional force microscopy

  Numata, Keiji; Sato, Shun; Fujita, Masahiro; Tsuge, Takeharu; Iwata, Tadahisa; Doi, Yoshiharu; Abe, Hideki

  Polymer Degradation and Stability, 2007

  
• Adsorption and hydrolysis reactions of poly(hydroxybutyric acid) depolymerases secreted from Ralstonia pickettii T1 and Penicillium funiculosum onto poly[(R)-3-hydroxybutyric acid]

  Numata, Keiji; Yamashita, Koichi; Fujita, Masahiro; Tsuge, Takeharu; Kasuya, Ken-ichi; Iwata, Tadahisa; Doi, Yoshiharu; Abe, Hideki

  Biomacromolecules, 2007

  
• Adsorption of biopolyester depolymerase on silicon wafer and poly[(R)-3-hydroxybutyric acid] single crystal revealed by real-time AFM

  Numata, K; Kikkawa, Y; Tsuge, T; Iwata, T; Doi, Y; Abe, H

  Macromolecular Bioscience, 2006

  
• Nonspecific hydrophobic interactions of a repressor protein, PhaR, with poly[(R)-3-hydroxybutyrate] film studied with a quartz crystal microbalance

  Yamashita, Koichi; Yamada, Miwa; Numata, Keiji; Taguchi, Seiichi

  Biomacromolecules, 2006

  
• Enzymatic degradation processes of poly[(R)-3-hydroxybutyric acid] and poly[(R)-3-hydroxybutyric acid-co-(R)-3-hydroxyvaleric acid] single crystals revealed by atomic force microscopy: Effects of molecular weight and second-monomer composition on erosion rates

  Numata, K; Kikkawa, Y; Tsuge, T; Iwata, T; Doi, Y; Abe, H

  Biomacromolecules, 2005

  
• Biosynthesis of polyhydroxyalkanoate (PHA) copolymer from fructose using wild-type and laboratory-evolved PHA synthases

  Tsuge, T; Yano, K; Imazu, S; Numata, K; Kikkawa, Y; Abe, H; Taguchi, S; Doi, Y

  Macromolecular Bioscience, 2005

  
• In-situ atomic force microscopy observation of enzymatic degradation in poly(hydroxyalkanoic acid) thin films: Normal and constrained conditions

  Kikkawa, Y; Hirota, T; Numata, K; Tsuge, T; Abe, H; Iwata, T; Doi, Y

  Macromolecular Bioscience, 2004

  
• Enzymatic degradation processes of lamellar crystals in thin films for poly[(R)-3-hydroxybutyric acid] and its copolymers revealed by real-time atomic force microscopy

  Numata, K; Hirota, T; Kikkawa, Y; Tsuge, T; Iwata, T; Abe, H; Doi, Y

  Biomacromolecules, 2004

  

Misc.

• Enzyme-like amidolytic catalysis from optimized poly(N-isopropylacrylamide) microgel

  Yoke Ming Wong; Yu Hoshino; Kumar Sudesh; Yoshiko Miura; Keiji Numata

  ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Aug. 2014

  
• Macromol. Biosci. 7/2016

  Kousuke Tsuchiya; Keiji Numata

  Macromolecular Bioscience, 01 Jul. 2016

  
• XFEL施設SACLAでの連続フェムト秒結晶構造解析における微結晶輸送媒体の利用

  菅原道泰; 菅原道泰; 南後恵理子; 南後恵理子; 田中智之; 田中智之; 山下鮎美; 山下鮎美; 有馬登志; 有馬登志; 田中里枝; 田中里枝; 沼田圭司; 岩田想; 岩田想

  日本結晶学会年会講演要旨集, 2017

  
• Synthesis of peptides with narrow molecular weight distributions via exopeptidase-catalyzed aminolysis of hydrophobic amino-acid alkyl esters

  Nitta Sachiko; Komatsu Ayaka; Ishii Taisei; Iwamoto Hiroyuki; Numata Keiji

  Polymer journal, Sep. 2016

  
• Aeromonas caviae由来ポリヒドロキシアルカン酸合成酵素の活性中間体に関する研究

  元田容子; 渡部暁; 栃尾尚哉; 木川隆則; 土肥義治; 沼田圭司

  高分子学会予稿集(CD-ROM), 2012

  
• ミトコンドリア-葉緑体間の相互作用解析

  及川和聡; 今井拓人; 児玉豊; 児玉豊; 沼田圭司

  日本植物生理学会年会(Web), 2019

  
• ペプチド-DNA複合体を用いた葉緑体形質転換法効率化の試み

  及川和聡; 児玉豊; 沼田圭司

  日本植物細胞分子生物学会大会・シンポジウム講演要旨集, 2018

  
• 細胞内分解システム抑制による葉緑体形質転換効率化の試み

  及川和聡; 児玉豊; 児玉豊; 沼田圭司

  日本植物生理学会年会(Web), 2018

  
• 化学酵素重合による天然を模倣したポリペプチド材料の合成 (特集 生体に優しく,賢い未来材料"生体適合材料")

  土屋 康佑; 沼田 圭司

  化学と工業 = Chemistry & chemical industry, Jun. 2017

  
• ペプチド-DNA複合体のオルガネラへの輸送過程を視る

  及川和聡; 児玉豊; 沼田圭司

  日本植物学会大会研究発表記録, 2017

  
• Poly(amino acid)s/polypeptides as potential functional and structural materials

  Numata Keiji

  Polymer journal, Aug. 2015

  
• 2P-121 Metabolic Engineering of Cyanobacteria with a Response Regulator Rre37

  Osanai Takashi; Oikawa Akira; Numata Keiji; Kuwahara Ayuko; Iijima Hiroko; Saito Kazuki; Hirai Masami

  日本生物工学会大会講演要旨集, 2013

  
• 2Ga08 Overexpression of SigE increases PHB production in cyanobacteria

  Osanai Takashi; Oikawa Akira; Numata Keiji; Toyooka Kiminori; Sato Mayuko; Kuwahara Ayuko; Iijima Hiroko; Doi Yoshiharu; Saito Kazuki; Hirai Masami

  日本生物工学会大会講演要旨集, 2012

  
• Silk-based delivery systems of bioactive molecules

  Numata, Keiji; Kaplan, David L.

  Advanced Drug Delivery Reviews, 2010

  

Books and Other Publications

• Biopolymer science for proteins and peptides

  沼田, 圭司, Single work

  Elsevier, 2021

  Find Kyoto University's collection of books :

  

Awards

• Jun. 2021

  繊維学会, 繊維学会賞
• Aug. 2020

  American Chemical Society, The 2020 ACS Macro Letters/ Biomacromolecules/Macromolecules Young Investigator Award
• Sep. 2019

  高分子学会, 旭化成賞
• Sep. 2019

  日本植物細胞分子生物学会, 日本植物細胞分子生物学会 奨励賞
• Aug. 2018

  BEPS 25th Anniversary Meeting, Bio-Environmental Polymer Society (BEPS), USA, The BEPS Outstanding Young Scientist Award
• Apr. 2018

  文部科学省, 文部科学大臣表彰若手科学者賞
• 2017

  Royal Society of Chemistry, UK, ChemComm Emerging Investigators
• 2016

  American Chemical Society,, The most highly prolific authors for Biomacromolecules
• 2015

  高分子学会 バイオ・高分子研究会, バイオ・高分子研究会 若手研究者奨励講演賞
• 2015

  高分子学会 関西支部, 第61回高分子研究発表会 ヤングサイエンティスト講演賞
• May 2014

  高分子学会, Polymer Journal 論文賞-日本ゼオン賞
• May 2014

  高分子学会, 高分子研究奨励賞
• 2012

  理化学研究所, 平成23年度 理化学研究所 研究奨励賞
• 2009

  the Council for the Lindau Nobel Laureate Meetings, リンダウノーベル賞受賞者会議（化学）派遣

External funds: Kakenhi

• 分解産物の精密解析と生物環境への影響評価

  Grant-in-Aid for Transformative Research Areas (B)

  Transformative Research Areas, Section (II)

  Kyoto University

  沼田 圭司

  From 02 Oct. 2020, To 31 Mar. 2023, Granted

  高分子の生分解;高分子精密分解;環境毒性;細胞毒性

  https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-20H05735/
• 精密高分子分解を志向した高分子解析基盤

  Grant-in-Aid for Transformative Research Areas (B)

  Transformative Research Areas, Section (II)

  Kyoto University

  沼田 圭司

  From 02 Oct. 2020, To 31 Mar. 2023, Granted

  高分子の精密分解;高分子精密分解

  https://kaken.nii.ac.jp/grant/KAKENHI-ORGANIZER-20H05732/
• Precision Degradation of Polymer and Polymeric Material

  Grant-in-Aid for Transformative Research Areas (B)

  From 02 Oct. 2020, To 31 Mar. 2023

  https://kaken.nii.ac.jp/grant/KAKENHI-AREA-20B203/
• Plant cell wall-mimicking silk-pectin hydrogel for coral reef regeneration

  Grant-in-Aid for Young Scientists (B)

  Institute of Physical and Chemical Research

  Keiji Numata

  From 01 Apr. 2013, To 31 Mar. 2016, Project Closed

  シルク;ネットワーク構造;サンゴ;ゲル;ベータシート構造;高分子材料;ペクチン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25810110/
• Gene carriers containing functional peptides for breast cancer treatment

  Grant-in-Aid for Young Scientists (B)

  The Institute of Physical and Chemical Research

  Keiji NUMATA

  From 01 Apr. 2011, To 31 Mar. 2013, Project Closed

  ペプチド;シルク;遺伝子デリバリー;癌細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23750264/
• Quantitative prediction of nucleic acid structures and functions affected by spaciotemporal environmental factors in cells

  Grant-in-Aid for Scientific Research (S)

  Broad Section E

  Konan University

  杉本 直己

  From 27 Apr. 2022, To 31 Mar. 2027, Granted

  核酸構造;細胞内環境;エネルギーデータベース;熱安定性予測;遺伝子発現

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H04975/

Education

Teaching subject(s)

• From 01 Apr. 2023, To 31 Mar. 2024

  Introduction to Industrial Chemistry

  7405, Spring, Faculty of Engineering, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Frontier Chemistry (Frontier Chemistry)

  7127, Spring, Faculty of Engineering, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Biorelated Material Chemistry

  7118, Spring, Faculty of Engineering, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Chemical Basis of Life

  7107, Spring, Faculty of Engineering, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Synthesis of Polymer Materials, Advanced

  S022, Fall, Graduate School of Engineering, 2
• From 01 Apr. 2023, To 31 Mar. 2024

  Frontiers in the Field of Chemical Biology and Biological Chemistry

  H409, Fall, Graduate School of Engineering, 1.5
• From 01 Apr. 2023, To 31 Mar. 2024

  Molecular Function and Composite-Assembly Function

  H404, Spring, Graduate School of Engineering, 1.5
• From 01 Apr. 2023, To 31 Mar. 2024

  Chemistry of Biomaterials

  H031, Fall, Graduate School of Engineering, 1.5
• From 01 Apr. 2023, To 31 Mar. 2024

  Chemistry of Polymer Materials

  H007, Spring, Graduate School of Engineering, 1.5
• From 01 Apr. 2022, To 31 Mar. 2023

  Chemistry of Polymer Materials

  H007, Spring, Graduate School of Engineering, 1.5
• From 01 Apr. 2022, To 31 Mar. 2023

  Material Chemistry Adv. II

  P056, Fall, Graduate School of Engineering, 0.5
• From 01 Apr. 2022, To 31 Mar. 2023

  Material Chemistry Adv. I

  P055, Spring, Graduate School of Engineering, 0.5
• From 01 Apr. 2022, To 31 Mar. 2023

  Master's Thesis

  D099, Year-long, Graduate School of Engineering, 0
• From 01 Apr. 2022, To 31 Mar. 2023

  General Material Chemistry

  P110, Spring, Graduate School of Engineering, 0.5
• From 01 Apr. 2022, To 31 Mar. 2023

  Introduction to Industrial Chemistry

  7405, Spring, Faculty of Engineering, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Laboratory and Exercise in Material Chemistry

  D037, Year-long, Graduate School of Engineering, 8
• From 01 Apr. 2022, To 31 Mar. 2023

  Dimensional Control and Micro-Nano Systems

  H403, Fall, Graduate School of Engineering, 1.5
• From 01 Apr. 2022, To 31 Mar. 2023

  Chemical Industry, Advanced

  P111, Spring, Graduate School of Engineering, 0.5
• From 01 Apr. 2022, To 31 Mar. 2023

  Synthesis of Polymer Materials, Advanced

  S022, Fall, Graduate School of Engineering, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Frontier Chemistry (Frontier Chemistry)

  7127, Spring, Faculty of Engineering, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Biorelated Material Chemistry

  7118, Spring, Faculty of Engineering, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Molecular Function and Composite-Assembly Function

  H404, Spring, Graduate School of Engineering, 1.5
• From Apr. 2021, To Mar. 2022

  Frontier Chemistry (Frontier Chemistry)

  Spring, 工学部
• From Apr. 2021, To Mar. 2022

  Frontiers in the Field of Chemical Biology and Biological Chemistry

  Fall, 工学研究科
• From Apr. 2021, To Mar. 2022

  Introduction to Engineering

  Spring, 工学部
• From Apr. 2021, To Mar. 2022

  Chemistry of Biomaterials

  Fall, 工学研究科
• From Apr. 2021, To Mar. 2022

  Biorelated Material Chemistry

  Spring, 工学部
• From Apr. 2021, To Mar. 2022

  Chemistry of Polymer Materials

  Spring, 工学研究科
• From Apr. 2021, To Mar. 2022

  Synthesis of Polymer Materials, Advanced

  Fall, 工学研究科

Participation in PhD Defense

• Development of analytical techniques for biomedical applications toward point-of-care testing devices

  Manmana Yanawut, Graduate School of Engineering, Sub-chief Examiner

  26 Sep. 2022

Administration

School management (title, position)

• From 01 Apr. 2022, To 31 Mar. 2024

  利益相反審査委員会 委員

Faculty management (title, position)

• From 01 Apr. 2021, To 31 Mar. 2023

  工学研究科・工学部広報委員会　委員

Academic, Social Contribution

Committee Memberships

• From 2022, To Present

  関西支部 幹事, 繊維学会
• From 2021, To Present

  ACS Polymers Au Editorial Advisory Board, American Chemical Society
• From 2021, To Present

  14. 羽ばたく女性研究者賞（マリア・スクウォドフスカ＝キュリー賞）（JST、ポーランド大使館） 選考委員, JST、ポーランド大使館
• From 2020, To Present

  CSJ化学フェスタ実行委員, 日本化学会
• From 2019, To Present

  Editorial Advisory Board of Biomacromolecules, American Chemical Society
• From 2019, To Present

  国際標準化委員会 委員, 一般社団法人 構造タンパク質素材産業推進協会
• From 2018, To Present

  バイオ高分子研究会 運営委員, 高分子学会
• From 2017, To Present

  Editorial Board of Scientific Reports, Springer-Nature
• From 2021, To 2028

  CREST研究領域「分解・劣化・安定化の精密材料科学」（研究総括：高原淳 九州大学特任教授）領域アドバイザー, JST
• From 2022, To 2027

  ACT-X研究領域「生命現象と機能性物質」（研究総括：豊島陽子 東京大学 教授）領域アドバイザー, JST
• From 2021, To 2023

  生体機能関連化学部会 幹事, 日本化学会
• From 2021, To 2022

  旭化成賞選考委員, 高分子学会