Basic Information

Affiliated programs (koza)

• Graduate School of Medicine, 医学専攻 細胞制御システム工学, 教授
• Graduate School of Medicine, 医科学専攻 細胞制御システム工学, 教授

Academic Degree

• 修士（工学）(東京大学)
• 博士（工学）(東京大学)

Research History

• From Apr. 2017

  京都大学iPS 細胞研究所, 副所長、学系長
• From Oct. 2014

  京都大学iPS 細胞研究所, 教授
• From Jul. 2011, To Sep. 2014

  京都大学iPS 細胞研究所, 特定准教授
• From Apr. 2010, To Sep. 2014

  京都大学白眉センター, 特定准教授
• From Apr. 2007, To Mar. 2010

  Kyoto University, Graduate School of Biostudies, 助教
• From Oct. 2005, To Mar. 2007

  Kyoto University, Graduate School of Biostudies, 助手
• From Apr. 2005, To Sep. 2005

  （財）癌研究会 癌研究所, 科学技術振興機構（JST）CREST 研究員
• From Apr. 2000, To Mar. 2005

  （財）癌研究会 癌研究所, SPD

ID,URL

• J-Global ID

  201401097295466016

• External link

  https://sites.google.com/view/hirohidesaitolabjp

Website(s) (URL(s))

• http://www.cira.kyoto-u.ac.jp/saito/
• https://sites.google.com/view/hirohidesaitolabjp

researchmap URL

• https://researchmap.jp/7000008581

Research

Research Topics, Overview of the research

• Research Topics

  Synthetic biology-based approaches to regulate cellular functions

• Overview of the research

  Research in the synthetic biology field, which attempts to bring about new technologies by understanding life through the process of “artificially creating” biomolecules and biological systems, is becoming established worldwide. Creating artificial biomolecules that freely control the functions of cells and applying them to examinations and medical treatments is one of the research goals of this new field. Our laboratory will use the unique technology of synthetic biology that molecularly designs RNA and/or RNA-protein complexes (RNP) artificially, assuming various functions in vivo, and experimentally evolve them* in order to develop a new technology that will allow the functions and fate of target cells to be freely controlled. Moreover, along with developing such new technologies, in order to understand the evolution/creation of biological systems, we aim to experimentally approach the fundamental questions of “What is life?” and “What are the structural principals of cells?”

Research Areas

• Life sciences, Systems genomics

Papers

• Split RNA switch: Programmable and precise control of gene expression by ensemble of pre- and post-translational regulation

  Itsuki Abe; Hirohisa Ohno; Megumi Mochizuki; Karin Hayashi; Hirohide Saito

  25 Sep. 2024

  
• Growth hormone enhances cellular elastase activity in circulating neutrophils, monocytes and lymphocytes

  T. Inoue; H. Saito

  Journal of Parenteral and Enteral Nutrition, 1998

  
• Purification technologies for induced pluripotent stem cell therapies

  Melanie Generali; Yoshihiko Fujita; Debora Kehl; Moe Hirosawa; Maximilian Y. Emmert; Jun Takahashi; Simon P. Hoerstrup; Hirohide Saito

  Nature Reviews Bioengineering, 22 Jul. 2024

  
• Folding RNA–Protein Complex into Designed Nanostructures

  Tomonori Shibata; Yuki Suzuki; Hiroshi Sugiyama; Masayuki Endo; Hirohide Saito

  Methods in Molecular Biology, 2021

  
• mRNA engineering for the control of mammalian cells in medical applications

  Kei Endo; Hirohide Saito

  Applied RNA Bioscience, 10 Apr. 2018

  
• Design of ligand-controlled genetic switches based on RNA interference

  Shunnichi Kashida; Hirohide Saito

  Synthetic Biology: Parts, Devices and Applications, 15 Mar. 2018

  
• Synthetic RNA–protein nanostructures and their potential applications

  Hirohisa Ohno; Eriko Osada; Tan Inoue; Hirohide Saito

  RNA Nanotechnology and Therapeutics, 01 Jan. 2013

  
• Programmable Computational RNA Droplets Assembled via Kissing-Loop Interaction

  Hirotake Udono; Minzhi Fan; Yoko Saito; Hirohisa Ohno; Shin-ichiro M. Nomura; Yoshihiro Shimizu; Hirohide Saito; Masahiro Takinoue

  ACS Nano, 03 Jun. 2024

  
• RNA Switches Using Cas Proteins.

  Moe Hirosawa; Hirohide Saito

  Methods in molecular biology (Clifton, N.J.), 2024

  
• Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules

  Shodai Komatsu; Hirohisa Ohno; Hirohide Saito

  Nature Communications, 17 Nov. 2023

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• Programmable mammalian translational modulators by CRISPR-associated proteins

  Shunsuke Kawasaki; Hiroki Ono; Moe Hirosawa; Takeru Kuwabara; Shunsuke Sumi; Suji Lee; Knut Woltjen; Hirohide Saito

  Nature Communications, 19 Apr. 2023

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• Versatile strategy using vaccinia virus-capping enzyme to synthesize functional 5' cap-modified mRNAs.

  Hirohisa Ohno; Sae Akamine; Megumi Mochizuki; Karin Hayashi; Shinichiro Akichika; Tsutomu Suzuki; Hirohide Saito

  Nucleic acids research, 03 Feb. 2023

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• Synthetic circular RNA switches and circuits that control protein expression in mammalian cells

  Shigetoshi Kameda; Hirohisa Ohno; Hirohide Saito

  Nucleic Acids Research, 16 Jan. 2023

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• Versatile Design of Intracellular Protein-Responsive Translational Regulation System for Synthetic mRNA.

  Hideyuki Nakanishi; Hirohide Saito; Keiji Itaka

  ACS synthetic biology, 18 Mar. 2022

  
• A versatile and robust cell purification system with an RNA-only circuit composed of microRNA-responsive ON and OFF switches.

  Yoshihiko Fujita; Moe Hirosawa; Karin Hayashi; Takeshi Hatani; Yoshinori Yoshida; Takuya Yamamoto; Hirohide Saito

  Science advances, 07 Jan. 2022

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• Purification of human iPSC-derived cells at large scale using microRNA switch and magnetic-activated cell sorting.

  Yuta Tsujisaka; Takeshi Hatani; Chikako Okubo; Ryo Ito; Azuma Kimura; Megumi Narita; Kazuhisa Chonabayashi; Shunsuke Funakoshi; Antonio Lucena-Cacace; Taro Toyoda; Kenji Osafune; Takeshi Kimura; Hirohide Saito; Yoshinori Yoshida

  Stem cell reports, 12 Jul. 2022

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• A protocol to construct RNA-protein devices for photochemical translational regulation of synthetic mRNAs in mammalian cells

  Hideyuki Nakanishi; Tatsuyuki Yoshii; Shinya Tsukiji; Hirohide Saito

  STAR Protocols, Jun. 2022

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• Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

  Mio Iwasaki; Yuka Kawahara; Chikako Okubo; Tatsuya Yamakawa; Michiko Nakamura; Tsuyoshi Tabata; Yohei Nishi; Megumi Narita; Akira Ohta; Hirohide Saito; Takuya Yamamoto; Masato Nakagawa; Shinya Yamanaka; Kazutoshi Takahashi

  iScience, 20 May 2022

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• Translational recoding by chemical modification of non-AUG start codon ribonucleotide bases.

  Yoshihiko Fujita; Takeru Kameda; Chingakham Ranjit Singh; Whitney Pepper; Ariana Cecil; Madelyn Hilgers; Mackenzie Thornton; Izumi Asano; Carter Moravek; Yuichi Togashi; Hirohide Saito; Katsura Asano

  Science advances, 08 Apr. 2022

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• Translational recoding by chemical modification of non-AUG start codon ribonucleotide bases

  Yoshihiko Fujita; Takeru Kameda; Chingakham Ranjit Singh; Whitney Pepper; Ariana Cecil; Madelyn Hilgers; Mackenzie Thornton; Izumi Asano; Carter Moravek; Yuichi Togashi; Hirohide Saito; Katsura Asano

  Science Advances, Apr. 2022

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• Synthetic RNA-based post-transcriptional expression control methods and genetic circuits.

  Malvin L Pardi; Juanqi Wu; Shunsuke Kawasaki; Hirohide Saito

  Advanced drug delivery reviews, 11 Mar. 2022

  
• An RNA Triangle with Six Ribozyme Units Can Promote a Trans-Splicing Reaction through Trimerization of Unit Ribozyme Dimers

  Junya Akagi; Takahiro Yamada; Kumi Hidaka; Yoshihiko Fujita; Hirohide Saito; Hiroshi Sugiyama; Masayuki Endo; Shigeyoshi Matsumura; Yoshiya Ikawa

  Applied Sciences, 14 Mar. 2021

  
• Light-controllable RNA-protein devices for translational regulation of synthetic mRNAs in mammalian cells.

  Hideyuki Nakanishi; Tatsuyuki Yoshii; Shunsuke Kawasaki; Karin Hayashi; Keita Tsutsui; Choji Oki; Shinya Tsukiji; Hirohide Saito

  Cell chemical biology, 20 Jan. 2021

  
• of Derived from Human Pluripotent Stem Using

  Kenji Miki; Hirohide Saito; Yoshinori Yoshida

  Methods in Molecular Biology, 2021

  
• Purification of Specific Cell Populations Differentiated from Stem Cells Using MicroRNA-Responsive Synthetic Messenger RNAs.

  Hideyuki Nakanishi; Hirohide Saito

  Methods in molecular biology (Clifton, N.J.), 2021

  
• RNA and protein-based nanodevices for mammalian post-transcriptional circuits.

  Shunsuke Kawasaki; Hiroki Ono; Moe Hirosawa; Hirohide Saito

  Current opinion in biotechnology, Jun. 2020, Peer-reviewed

  
• Synthetic mRNA-Based Systems in Mammalian Cells.

  Hirohisa Ohno; Sae Akamine; Hirohide Saito

  Advanced biosystems, May 2020, Peer-reviewed

  
• N 1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells.

  Callum J C Parr; Shunsuke Wada; Kenjiro Kotake; Shigetoshi Kameda; Satoshi Matsuura; Souhei Sakashita; Soyoung Park; Hiroshi Sugiyama; Yi Kuang; Hirohide Saito

  Nucleic acids research, 06 Apr. 2020, Peer-reviewed

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• Caliciviral protein-based artificial translational activator for mammalian gene circuits with RNA-only delivery.

  Hideyuki Nakanishi; Hirohide Saito

  Nature communications, 10 Mar. 2020, Peer-reviewed

  
• Orthogonal Protein-Responsive mRNA Switches for Mammalian Synthetic Biology.

  Hiroki Ono; Shunsuke Kawasaki; Hirohide Saito

  ACS synthetic biology, 17 Jan. 2020, Peer-reviewed

  
• Editorial overview: Mammalian synthetic biology: from devices to multicellular systems.

  Hirohide Saito; Yohei Yokobayashi

  Current opinion in chemical biology, Oct. 2019, Peer-reviewed

  
• Mammalian gene circuits with biomolecule-responsive RNA devices.

  Hideyuki Nakanishi; Hirohide Saito

  Current opinion in chemical biology, Oct. 2019, Peer-reviewed

  
• Numerical operations in living cells by programmable RNA devices.

  Kei Endo; Karin Hayashi; Hirohide Saito

  Science advances, Aug. 2019, Peer-reviewed

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• RNA nanostructures and scaffolds for biotechnology applications.

  Hirohisa Ohno; Sae Akamine; Hirohide Saito

  Current opinion in biotechnology, Aug. 2019, Peer-reviewed

  
• Cell-Type-Specific CRISPR Activation with MicroRNA-Responsive AcrllA4 Switch.

  Moe Hirosawa; Yoshihiko Fujita; Hirohide Saito

  ACS synthetic biology, 19 Jul. 2019, Peer-reviewed

  
• Nanoparticle-based local translation reveals mRNA as a translation-coupled scaffold with anchoring function.

  Shunnichi Kashida; Dan Ohtan Wang; Hirohide Saito; Zoher Gueroui

  Proceedings of the National Academy of Sciences of the United States of America, 02 Jul. 2019, Peer-reviewed

  
• Author Correction: Synthetic RNA-based logic computation in mammalian cells.

  Satoshi Matsuura; Hiroki Ono; Shunsuke Kawasaki; Yi Kuang; Yoshihiko Fujita; Hirohide Saito

  Nature communications, 26 Apr. 2019, Peer-reviewed

  
• MicroRNA-Based Separation of Cortico-Fugal Projection Neuron-Like Cells Derived From Embryonic Stem Cells.

  Tadashi Sunohara; Asuka Morizane; Satoshi Matsuura; Susumu Miyamoto; Hirohide Saito; Jun Takahashi

  Frontiers in neuroscience, 2019, Peer-reviewed

  
• Synthetic RNA-based logic computation in mammalian cells.

  Satoshi Matsuura; Hiroki Ono; Shunsuke Kawasaki; Yi Kuang; Yoshihiko Fujita; Hirohide Saito

  Nature communications, 19 Nov. 2018, Peer-reviewed

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• Efficient mRNA delivery system utilizing chimeric VSVG-L7Ae virus-like particles.

  Yulia Zhitnyuk; Peter Gee; Mandy S Y Lung; Noriko Sasakawa; Huaigeng Xu; Hirohide Saito; Akitsu Hotta

  Biochemical and biophysical research communications, 10 Nov. 2018, Peer-reviewed

  
• Competition between translation initiation factor eIF5 and its mimic protein 5MP determines non-AUG initiation rate genome-wide.

  Leiming Tang; Jacob Morris; Ji Wan; Chelsea Moore; Yoshihiko Fujita; Sarah Gillaspie; Eric Aube; Jagpreet Nanda; Maud Marques; Maika Jangal; Abbey Anderson; Christian Cox; Hiroyuki Hiraishi; Leiming Dong; Hirohide Saito; Chingakham Ranjit Singh; Michael Witcher; Ivan Topisirovic; Shu-Bing Qian; Katsura Asano

  Nucleic acids research, 16 Nov. 2017, Peer-reviewed

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• Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate.

  Tomonori Shibata; Yoshihiko Fujita; Hirohisa Ohno; Yuki Suzuki; Karin Hayashi; Kaoru R Komatsu; Shunsuke Kawasaki; Kumi Hidaka; Shin Yonehara; Hiroshi Sugiyama; Masayuki Endo; Hirohide Saito

  Nature communications, 14 Sep. 2017, Peer-reviewed

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• Cell-type-specific genome editing with a microRNA-responsive CRISPR-Cas9 switch.

  Moe Hirosawa; Yoshihiko Fujita; Callum J C Parr; Karin Hayashi; Shunnichi Kashida; Akitsu Hotta; Knut Woltjen; Hirohide Saito

  Nucleic acids research, 27 Jul. 2017, Peer-reviewed

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• Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells.

  Shunsuke Kawasaki; Yoshihiko Fujita; Takashi Nagaike; Kozo Tomita; Hirohide Saito

  Nucleic acids research, 07 Jul. 2017, Peer-reviewed

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• Efficient, Selective Removal of Human Pluripotent Stem Cells via Ecto-Alkaline Phosphatase-Mediated Aggregation of Synthetic Peptides.

  Yi Kuang; Kenji Miki; Callum J C Parr; Karin Hayashi; Ikue Takei; Jie Li; Mio Iwasaki; Masato Nakagawa; Yoshinori Yoshida; Hirohide Saito

  Cell chemical biology, 22 Jun. 2017, Peer-reviewed

  
• Efficient, Selective Removal of Human Pluripotent Stem Cells via Ecto-Alkaline Phosphatase-Mediated Aggregation of Synthetic Peptides

  Yi Kuang; Kenji Miki; Callum J. C. Parr; Karin Hayashi; Ikue Takei; Jie Li; Mio Iwasaki; Masato Nakagawa; Yoshinori Yoshida; Hirohide Saito

  CELL CHEMICAL BIOLOGY, Jun. 2017, Peer-reviewed

  
• Monitoring and visualizing microRNA dynamics during live cell differentiation using microRNA-responsive non-viral reporter vectors

  Hideyuki Nakanishi; Kenji Miki; Kaoru R. Komatsu; Masayuki Umeda; Megumi Mochizuki; Azusa Inagaki; Yoshinori Yoshida; Hirohide Saito

  BIOMATERIALS, Jun. 2017, Peer-reviewed

  
• RNA-based gene circuits for cell regulation

  Peter Karagiannis; Yoshihiko Fujita; Hirohide Saito

  PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, Nov. 2016, Peer-reviewed

  
• MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells

  Callum J. C. Parr; Shota Katayama; Kenji Miki; Yi Kuang; Yoshinori Yoshida; Asuka Morizane; Jun Takahashi; Shinya Yamanaka; Hirohide Saito

  SCIENTIFIC REPORTS, Sep. 2016, Peer-reviewed

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• High-resolution Identification and Separation of Living Cell Types by Multiple microRNA-responsive Synthetic mRNAs

  Kei Endo; Karin Hayashi; Hirohide Saito

  SCIENTIFIC REPORTS, Feb. 2016, Peer-reviewed

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• RNA/RNP Nanotechnology for Biological Applications

  OHNO Hirohisa; SAITO Hirohide

  Seibutsu Butsuri, 2016, Peer-reviewed, Invited

  
• RNA and RNP as Building Blocks for Nanotechnology and Synthetic Biology

  Hirohisa Ohno; Hirohide Saito

  NANOTECHNOLOGY TOOLS FOR THE STUDY OF RNA, 2016, Peer-reviewed

  
• Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions

  Masamune Morita; Hiroaki Onoe; Miho Yanagisawa; Hiroaki Ito; Masatoshi Ichikawa; Kei Fujiwara; Hirohide Saito; Masahiro Takinoue

  CHEMBIOCHEM, Sep. 2015, Peer-reviewed

  
• Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery

  Liliana Wroblewska; Tasuku Kitada; Kei Endo; Velia Siciliano; Breanna Stillo; Hirohide Saito; Ron Weiss

  NATURE BIOTECHNOLOGY, Aug. 2015, Peer-reviewed

  
• Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches

  Kenji Miki; Kei Endo; Seiya Takahashi; Shunsuke Funakoshi; Ikue Takei; Shota Katayama; Taro Toyoda; Maki Kotaka; Tadashi Takaki; Masayuki Umeda; Chikako Okubo; Misato Nishikawa; Akiko Oishi; Megumi Narita; Ito Miyashita; Kanako Asano; Karin Hayashi; Kenji Osafune; Shinya Yamanaka; Hirohide Saito; Yoshinori Yoshida

  CELL STEM CELL, Jun. 2015, Peer-reviewed

  
• Synthetic RNA Technologies to Control Functions of Mammalian Cells

  Hirohide Saito

  YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, Mar. 2015, Peer-reviewed

  
• Folding RNA-Protein Complex into Designed Nanostructures.

  Tomonori Shibata; Yuki Suzuki; Hiroshi Sugiyama; Masayuki Endo; Hirohide Saito

  Methods in molecular biology (Clifton, N.J.), 2015, Peer-reviewed

  
• Design, assembly, and evaluation of RNA-protein nanostructures.

  Hirohisa Ohno; Eriko Osada; Hirohide Saito

  Methods in molecular biology (Clifton, N.J.), 2015, Peer-reviewed

  
• Expanding the synthetic ribonucleoprotein world in cells

  Kei Endo; Callum Parr; Hirohide Saito

  NATURE METHODS, Nov. 2014, Peer-reviewed

  
• The rapid synthesis of cell-sized liposomes by centrifuge-based microfluidic device

  Masamune Morita; Hiroaki Onoe; Miho Yanagisawa; Kei Fujiwara; Hirohide Saito; Masahiro Takinoue

  Proceedings of The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS2014), Oct. 2014, Peer-reviewed

  
• Engineering RNA-Protein Complexes with Different Shapes for Imaging and Therapeutic Applications

  Eriko Osada; Yuki Suzuki; Kumi Hidaka; Hirohisa Ohno; Hiroshi Sugiyama; Masayuki Endo; Hirohide Saito

  ACS NANO, Aug. 2014, Peer-reviewed

  
• Molecular Robots with Sensors and Intelligence

  Masami Hagiya; Akihiko Konagaya; Satoshi Kobayashi; Hirohide Saito; Satoshi Murata

  ACCOUNTS OF CHEMICAL RESEARCH, Jun. 2014, Peer-reviewed

  
• 3SAA-03 Designing DNA/RNA nanostructure-based information converters(Development of Molecular Robots equipped with Sensors and Intelligence,Symposium,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

  Saito Hirohide; Endo Masayuki; Takinoue Masahiro

  Seibutsu Butsuri, 2014

  
• 1P213 Artificial cell-based molecular robots by fusion of light responsive DNA and cell-sized liposomes(13B. Biological & Artificial membrane: Dynamics,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

  Morita Masamune; Li Hao; Shibata Tomonori; Saito Hirohide; Takinoue Masahiro

  Seibutsu Butsuri, 2014

  
• RNA-Protein nanoteclmology & synthetic biology

  Hirohide Saito

  Seikagaku, 2014, Peer-reviewed

  
• A three-dimensional design strategy for a protein-responsive shRNA switch

  Shunnichi Kashida; Hirohide Saito

  Methods in Molecular Biology, 01 Jan. 2014, Peer-reviewed

  
• Engineering protein-responsive mRNA switch in mammalian cells

  Kei Endo; Hirohide Saito

  Methods in Molecular Biology, 01 Jan. 2014, Peer-reviewed

  
• A versatile cis-acting inverter module for synthetic translational switches

  Kei Endo; Karin Hayashi; Tan Inoue; Hirohide Saito

  NATURE COMMUNICATIONS, Sep. 2013, Peer-reviewed

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• Quantitative and simultaneous translational control of distinct mammalian mRNAs

  Kei Endo; James A. Stapleton; Karin Hayashi; Hirohide Saito; Tan Inoue

  Nucleic Acids Research, Jul. 2013, Peer-reviewed

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• Molecular Robotics: A New Paradigm for Artifacts

  Satoshi Murata; Akihiko Konagaya; Satoshi Kobayashi; Hirohide Saito; Masami Hagiya

  NEW GENERATION COMPUTING, Jan. 2013, Peer-reviewed

  
• Directed evolution of a synthetic RNA-protein module to create a new translational switch

  Tomoaki Hara; Hirohide Saito; Tan Inoue

  CHEMICAL COMMUNICATIONS, 2013, Peer-reviewed

  
• Three-dimensionally designed protein-responsive RNA devices for cell signaling regulation

  Shunnichi Kashida; Tan Inoue; Hirohide Saito

  NUCLEIC ACIDS RESEARCH, Oct. 2012, Peer-reviewed

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• Feedback Control of Protein Expression in Mammalian Cells by Tunable Synthetic Translational Inhibition

  James A. Stapleton; Kei Endo; Yoshihiko Fujita; Karin Hayashi; Masahiro Takinoue; Hirohide Saito; Tan Inoue

  ACS SYNTHETIC BIOLOGY, Mar. 2012, Peer-reviewed

  
• Erratum: Synthetic human cell fate regulation by protein-driven RNA switches (Nature Communications (2011) 2 (160) DOI: 10.1038/ncomms1157)

  H. Saito; Y. Fujita; S. Kashida; K. Hayashi; T. Inoue

  Nature Communications, 2012, Peer-reviewed

  
• Light-Regulated mRNA Condensation by a Photosensitive Surfactant Works as a Series Photoswitch of Translation Activity in the Presence of Small RNAs

  Sergii Rudiuk; Hirohide Saito; Tomoaki Hara; Tan Inoue; Kenichi Yoshikawa; Damien Baigl

  BIOMACROMOLECULES, Nov. 2011, Peer-reviewed

  
• Self-Replication Reactions Dependent on Tertiary Interaction Motifs in an RNA Ligase Ribozyme

  Rei Ohmori; Hirohide Saito; Yoshiya Ikawa; Yoshihiko Fujita; Tan Inoue

  JOURNAL OF MOLECULAR EVOLUTION, Oct. 2011, Peer-reviewed

  
• Synthetic RNA-protein complex shaped like an equilateral triangle

  Hirohisa Ohno; Tetsuhiro Kobayashi; Rinko Kabata; Kei Endo; Takuma Iwasa; Shige H. Yoshimura; Kunio Takeyasu; Tan Inoue; Hirohide Saito

  NATURE NANOTECHNOLOGY, Feb. 2011, Peer-reviewed

  
• Synthetic human cell fate regulation by protein-driven RNA switches

  Hirohide Saito; Yoshihiko Fujita; Shunnichi Kashida; Karin Hayashi; Tan Inoue

  NATURE COMMUNICATIONS, Jan. 2011, Peer-reviewed

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• Synthetic translational regulation by an L7Ae-kink-turn RNP switch

  Hirohide Saito; Tetsuhiro Kobayashi; Tomoaki Hara; Yoshihiko Fujita; Karin Hayashi; Rie Furushima; Tan Inoue

  NATURE CHEMICAL BIOLOGY, Jan. 2010, Peer-reviewed

  
• Coordinated control of a designed trans-acting ligase ribozyme by a loop-receptor interaction

  Shigeyoshi Matsumura; Rei Ohmori; Hirohide Saito; Yoshiya Ikawa; Tan Inoue

  FEBS LETTERS, Sep. 2009, Peer-reviewed

  
• Time-Resolved Tracking of a Minimum Gene Expression System Reconstituted in Giant Liposomes

  Hirohide Saito; Yusho Kato; Mael Le Berre; Ayako Yamada; Tan Inoue; Kenichi Yosikawa; Damien Baigl

  CHEMBIOCHEM, Jul. 2009, Peer-reviewed

  
• A designed RNA selection: establishment of a stable complex between a target and selectant RNA via two coordinated interactions

  Tomoaki Shiohara; Hirohide Saito; Tan Inoue

  NUCLEIC ACIDS RESEARCH, Feb. 2009, Peer-reviewed

  
• Synthetic biology with RNA motifs

  Hirohide Saito; Tan Inoue

  INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Feb. 2009, Peer-reviewed

  
• Motif-programmed artificial protein induces apoptosis in several cancer cells by disrupting mitochondria

  Hirohide Saito; Tamiko Minamisawa; Takao Yamori; Kiyotaka Shiba

  CANCER SCIENCE, Feb. 2008, Peer-reviewed

  
• The role of peptide motifs in the evolution of a protein network

  Hirohide Saito; Shunnichi Kashida; Tan Inoue; Kiyotaka Shiba

  NUCLEIC ACIDS RESEARCH, Oct. 2007, Peer-reviewed

  
• RNA and RNP as new molecular parts in synthetic biology

  Hirohide Saito; Tan Inoue

  JOURNAL OF BIOTECHNOLOGY, Oct. 2007, Peer-reviewed

  
• Motif programming: a microgene-based method for creating synthetic proteins containing multiple functional motifs

  Hirohide Saito; Tamiko Minamisawa; Kiyotaka Shiba

  NUCLEIC ACIDS RESEARCH, Mar. 2007, Peer-reviewed

  
• Designer ribozymes: Programming the tRNA specificity into flexizyme

  K Ramaswamy; H Saito; H Murakami; K Shiba; H Suga

  JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Sep. 2004, Peer-reviewed

  
• Synthesis of functional proteins by mixing peptide motifs

  H Saito; T Honma; T Minamisawa; K Yamazaki; T Noda; T Yamori; K Shiba

  CHEMISTRY & BIOLOGY, Jun. 2004, Peer-reviewed

  
• A versatile tRNA aminoacylation catalyst based on RNA

  H Murakami; H Saito; H Suga

  CHEMISTRY & BIOLOGY, Jul. 2003, Peer-reviewed

  
• Outersphere and innersphere coordinated metal ions in an aminoacyl-tRNA synthetase ribozyme

  H Saito; H Suga

  NUCLEIC ACIDS RESEARCH, Dec. 2002, Peer-reviewed

  
• Searching the origin of life by in vitro evolution system

  H. Saito; K. Watanabe; S. Hiroaki

  Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 2002, Peer-reviewed

  
• Concurrent molecular recognition of the amino acid and tRNA by a ribozyme

  H Saito; K Watanabe; H Suga

  RNA-A PUBLICATION OF THE RNA SOCIETY, Dec. 2001, Peer-reviewed

  
• A ribozyme exclusively aminoacylates the 3 '-hydroxyl group of the tRNA terminal adenosine

  H Saito; H Suga

  JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Jul. 2001, Peer-reviewed

  
• An in vitro evolved precursor tRNA with aminoacylation activity

  H Saito; D Kourouklis; H Suga

  EMBO JOURNAL, Apr. 2001, Peer-reviewed

  

Misc.

• RNAを用いた高効率なヒトiPS細胞の作製に向けて

  山縣茉莉; 山縣茉莉; 弘澤萌; 藤田祥彦; 齊藤博英

  日本分子生物学会年会プログラム・要旨集(Web), 2019

  
• RNAを用いた高効率なiPS細胞の作製に向けて

  山縣茉莉; 山縣茉莉; 弘澤萌; 弘澤萌; 松浦理史; 松浦理史; 藤田祥彦; 齊藤博英

  日本分子生物学会年会プログラム・要旨集(Web), 2018

  
• 人工RNAを基盤としたCRISPR/Cas9Systemの構築

  弘澤萌; 弘澤萌; 樫田俊一; 中村正裕; LI Hongmei; 堀田秋津; 渡辺亮; 齊藤博英

  日本RNA学会年会要旨集, 2015

  
• モチーフ・プログラミング--ペプチドモチーフの組み合わせから人工タンパク質を創出する第二世代進化分子工学 (特集1 マテリアルサイエンスで活躍するタンパク質(part2))

  齊藤 博英; 芝 清隆

  バイオインダストリー, Nov. 2005

  
• Endogenous Signal-Responsive Transgene Switch Systems for Visualization and Purification of Specific Cells

  Hideyuki Nakanishi; Hirohide Saito

  Medical Applications of iPS Cells, May 2019, Peer-reviewed, Invited

  
• RNA-タンパク質間相互作用を利用したプログラムと拡張が可能な細胞内コンピューティング

  小野紘貴; 小野紘貴; 川崎俊輔; 弘澤萌; 齊藤博英

  日本分子生物学会年会プログラム・要旨集(Web), 2019

  
• 翻訳制御に基づく哺乳類細胞コンピュータ

  川崎俊輔; 小野紘貴; 小野紘貴; 弘澤萌; 齊藤博英

  日本分子生物学会年会プログラム・要旨集(Web), 2019

  
• mRNAスイッチによる細胞内シグナルの検出と運命制御

  川崎俊輔; 齊藤博英

  日本分子生物学会年会プログラム・要旨集(Web), 2018

  
• Visualization and selection of target cells using microRNA-responsive switch system

  Hideyuki Nakanishi; Hirohide Saito

  Bioscience & Industry, Nov. 2017, Peer-reviewed, Invited

  
• An update on stem cell biology and engineering for brain development

  C. J.C. Parr; S. Yamanaka; H. Saito

  Molecular Psychiatry, 01 Jun. 2017

  
• Synthetic miRNA Switch Technology Elucidates Heterogeneity in Regulation of Immortalized Megakaryocyte Cell Lines, Associated with Improvement of Platelet Generation Efficiency for Clinical Use

  Kazuya Hashimoto; Satoshi Matsuura; Yoshihiko Fujita; Karin Hayashi; Naoshi Sugimoto; Takuya Yamamoto; Hirohide Saito; Koji Eto

  BLOOD, Dec. 2016

  
• 哺乳類細胞における人工遺伝子ネットワークの構築

  中西秀之; 齊藤博英

  生物工学会誌, Oct. 2015, Peer-reviewed, Invited

  
• Synthetic Biology of Translational Regulation

  藤田 祥彦; 齊藤 博英

  細胞工学, 2015

  
• A Novel Purification Method for Cardiomyocytes and Endothelial Cells Derived From Human Pluripotent Stem Cells Using Micrornaresponsive Messenger Rna

  Kenji Miki; Seiya Takahashi; Shunsuke Funakoshi; Shinya Yamanaka; Hirohide Saito; Yoshinori Yoshida

  CIRCULATION, Nov. 2014

  
• DESIGN OF PROTEIN-RESPONSIVE shRNA SYSTEM FOR TRANSLATIONAL CONTROL IN HUMAN CELL

  Tan Inoue; Shunichi Kashida; Yoshihiko Fujita; Hirohide Saito

  NUCLEIC ACID THERAPEUTICS, Oct. 2011

  
• Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder

  Andre Estevez-Torres; Cecile Crozatier; Antoine Diguet; Tomoaki Hara; Hirohide Saito; Kenichi Yoshikawa; Damien Baigl

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jul. 2009, Peer-reviewed

  
• 生命システムの制御・再構成にむけた合成的アプローチ

  齊藤博英; 井上丹; 芝清隆

  生化学, 2007

  
• Towards constructing synthetic cells: RNA/RNP evolution and cell-free translational systems in giant liposomes

  Hirohide Saito; Ayako Yamada; Rei Ohmori; Yusho Kato; Toru Yamanaka; Kenichi Yoshikawa; Tan Inoue

  2007 INTERNATIONAL SYMPOSIUM ON MICRO-NANO MECHATRONICS AND HUMAN SCIENCE, VOLS 1 AND 2, 2007, Peer-reviewed

  
• A synthetic approach for protein evolution and cell engineering

  Hirohide Saito; Tan Inoue; Kiyotaka Shiba

  2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS, 2006, Peer-reviewed

  
• 試験管内RNA‐Proteinワールドの創出にむけて

  斉藤博英; 菅裕明; 芝清隆

  生化学, 25 Aug. 2002

  

Presentations

• RNP 合成生物学：哺乳類細胞への応用

  Hirohide Saito

  第８回合成生物工学シンポジウム, 26 Sep. 2018, 神戸大学バイオプロダクション次世代農工連携拠点, Invited

  
• 研究者として生きる

  Hirohide Saito

  平成30年度 教育講演会, 21 Sep. 2018, 福井大学教育学部附属義務教育学校後期課程, Invited

  
• RNA synthetic biology and nanotechnology to program cells

  Hirohide Saito

  生物物理学会第56回, 15 Sep. 2018, 生物物理学会

  
• 未来医療を加速するRNAスイッチ ・テクノロジー

  Hirohide Saito

  1st Inovation Forum Japan, 14 Sep. 2018, ザルトリウス・ステディム・ジャパン株式会社, Invited

  
• Synthetic RNA technologies to program cells

  Hirohide Saito

  Synthetic Biology: Engineering Life 2.0 2018, 05 Sep. 2018, 神戸大学

  
• 研究者として生きる

  Hirohide Saito

  岸和田高校 出張講義, 24 Aug. 2018, 岸和田高校, Invited

  
• RNA-スイッチ技術と今年設立された『株式会社aceRNATechnologies』について研究者の立場から

  Hirohide Saito

  第4回KRICフォーラム, 03 Aug. 2018, 近畿経済産業局 関西再生医療産業コンソーシアム(KRIC), Invited

  
• 人工RNA技術を活用した遺伝子操作と細胞運命の制御

  Hirohide Saito

  生物化学セミナー, 27 Jul. 2018, 東京大学 理学部生物化学科・濡木研究室, Invited

  
• Synthetic RNA technologies to control mammalian cell fate

  Hirohide Saito

  Preliminary Program BZH Meeting 2018, 15 Jul. 2018, BZH, Invited

  
• Synthetic RNA switch technologies for programming cell fate

  Hirohide Saito

  Zoher GUEROUI Laboratory, Department of Chemistry,Ecole Normale Sup_rieure, Paris, 11 Jul. 2018, Ecole Normale Sup_rieure, Paris

  
• Synthetic RNA switch technologies to purify and program target cell types of Ipsc

  Hirohide Saito

  Frontier iPS and Stem Cell Therapeutic Summit 2018 in Taipei, 28 Jun. 2018, 台北栄民総医院, Invited

  
• 未来医療を加速するRNAスイッチ・テクノロジー

  Hirohide Saito

  第55回薬剤学懇談会研究討論会, 14 Jun. 2018, 武田薬品工業株式会社, Invited

  
• 科学者という生き方

  Hirohide Saito

  錦林小学校講演, 12 Jun. 2018, 錦林小学校, Invited

  
• 人工RNAシステムによるゲノム編集と細胞運命の制御

  Hirohide Saito

  東京化学会議, 01 Jun. 2018, 日本化学会, Invited

  
• "RNA合成生物学を活用した 細胞機能の制御"

  Hirohide Saito

  名古屋大学講義, 25 May 2018, 名古屋大学, Invited

  
• 研究者を目指したきっかけ：中学時代を振り返って

  Hirohide Saito

  近衛中学 第1回PTA家庭教育講座, 22 May 2018, 近衛中学PTA, Invited

  
• 人工RNAシステムによるゲノム編集と細胞運命の制御

  Hirohide Saito

  第82回 日本生化学会 中部支部 例会・シンポジウム, 19 May 2018, 岐阜大学, Invited

  
• RNA分子ロボティクスのiPS細胞への活用

  Hirohide Saito

  CBI学会, 18 May 2018, 情報計算化学生物学会（CBI学会）関西部会, Invited

  
• 人工RNAシステムによる細胞の識別と運命の制御

  Hirohide Saito

  日本薬学会第138年会, 26 Mar. 2018, Invited

  
• Expanding synthetic RNP world in cells

  Hirohide Saito

  CDBリトリート, 19 Mar. 2018, Invited

  
• Synthetic RNA technologies to program cells

  Hirohide Saito

  The SPIRITS INTERNATIONAL SYMPOSIUM 2018, 27 Feb. 2018, Invited

  
• RNAスイッチ：標的細胞の自在な選別と運命制御

  Hirohide Saito

  平成29年度 第6回 再生医療サポートビジネス講話会, 22 Feb. 2018, Invited

  
• RNAタンパク質複合体(RNP)研究

  Hirohide Saito

  エーザイ筑波サイエンス道場, 20 Feb. 2018, Invited

  
• Synthetic RNA switch technologies to purify and program target cell types

  Hirohide Saito

  14th US-Japan Symposium on Drag Delivery Systems, 17 Dec. 2017, Invited

  
• RNAナノデバイスによる細胞プログラミング

  Hirohide Saito

  第30回バイオエンジニアリング講話会, 14 Dec. 2017, Invited

  
• Synthetic RNA switch technologies to purify and program target cell types

  Hirohide Saito

  CiRA国際シンポジウム, 06 Nov. 2017, Invited

  
• iPS細胞とは何か〜臨床応用への最前線

  Hirohide Saito

  長崎総合化学大学 公開講演会, 05 Nov. 2017, Invited

  
• iPS細胞研究と教育の効果

  Hirohide Saito

  第28回近畿ブロック国際理解教育研究会, 28 Oct. 2017, Invited

  
• 細胞の運命を変える研究：iPS細胞とRNAの不思議にせまる

  Hirohide Saito

  岸和田高校 出張講義, 23 Aug. 2017, Invited

  
• RNAナノテクノロジーを活用した細胞運命の人為的制御法の開発

  Hirohide Saito

  第6回「産業基盤の創生」研究成果発表プログラム, 08 Aug. 2017, Invited

  
• RNAスイッチによる細胞運命制御

  Hirohide Saito

  川西市生涯学習短期大学, 28 Jul. 2017, Invited

  
• RNAスイッチとiPS細胞研究への応用

  Hirohide Saito

  富士フイルム創薬セミナー, 25 Jul. 2017, Invited

  
• RNAスイッチによるiPS細胞分離選択技術と創薬

  Hirohide Saito

  東京大学薬学部 ヒト細胞創薬学寄附講座, 24 Jul. 2017, Invited

  
• Synthetic RNA switches & nanodevices for cell programming

  Hirohide Saito

  FIBER International summit for nucleic acids2017, 19 Jul. 2017, Invited

  
• RNAスイッチのデザインと応用

  Hirohide Saito

  第33回日本DDS学会学術集会, 06 Jul. 2017, Invited

  
• Synthetic RNA switch technologies to Distinguish and program Living cells

  Hirohide Saito

  内藤カンファレンス, 27 Jun. 2017, Invited

  
• RNAテクノロジーを活用した細胞運命制御

  Hirohide Saito

  東京大学 システム生物医学セミナー, 19 May 2017, Invited

  
• Synthetic RNA switches and nanodevices for cell programming

  Hirohide Saito

  OISTセミナー, 18 Apr. 2017, Invited

  
• Biophysics and Physicobiology

  Hirohide Saito

  生物物理学会, 2017, Invited

  
• Synthetic cell programming

  Hirohide Saito

  Malaysian Society for stem Cell Research and Therapy, 17 Nov. 2016, Invited

  
• iPS細胞とRNAスイッチー研究の展望と医療への可能

  Hirohide Saito

  医学部進学フォーラム2016, 19 Sep. 2016, Invited

  
• iPS細胞とRNAスイッチ：目的の細胞をつくることは可能か？

  Hirohide Saito

  平成28年 沼津市民大学, 06 Aug. 2016, Invited

  
• Protein-driven nanostructures:Controlling mammalian cell fate

  Hirohide Saito

  RNA Naonotechnology Conference, 01 Aug. 2016, Invited

  
• Synthetic RNA switches & nanostructures that Indentify and control target mammmalian cells based n intracelluar information

  Hirohide Saito

  FIBER International summit for nucleic acids2016, 16 Jul. 2016, Invited

  
• Synthetic RNA switches & nanostructures

  Hirohide Saito

  RNA2016 satellite-symposium 'Nascent biology and ribosome function, 27 Jun. 2016, Invited

  
• MicroRNA SWITCHES”_THAT IDENTIFY AND ISOLATE TARGET CELLS_IN_HIGH-RESOLUTION

  Hirohide Saito

  ISSCR, 22 Jun. 2016, Invited

  
• Synthetic RNA switches & nanostructures: programming mammalian cell fate

  Hirohide Saito

  19 Jun. 2016, Invited

  
• Synthetic RNA Switches and Circuits to Program Target Cell Populations

  Hirohide Saito

  mSBW3.0, 21 May 2016, Invited

  
• Development of Molecular Sensor and Device for Intelligent Molecular Robots

  Hirohide Saito

  IEEE-NEMS2016, 17 Apr. 2016, Invited

  
• Synthetic RNA Switches and Circuits to Detect and Purify Target Live Cells

  Hirohide Saito

  UC San Diego Kyoto University joint Sympojium, 13 Mar. 2016, Invited

  

Awards

• Jul. 2002

  フジサンケイビジネス社, 独創性を拓く先端科学技術論文 第16回文部科学大臣賞 受賞 　　　　　　　(学生の部最優秀賞）
• Dec. 2012

  日本分子生物学会, 第10回日本分子生物学会三菱化学奨励賞
• Apr. 2013

  文部科学省, 平成25年度文部科学大臣表彰 若手科学者賞
• Jan. 2015

  日本学術振興会, 平成26年度日本学術振興会賞
• Apr. 2015

  公益財団法人 中谷医工計測技術振興財団, 平成26年度中谷賞奨励賞

External funds: Kakenhi

• RNA-based Synthetic Life Systems

  Grant-in-Aid for Specially Promoted Research

  Biological Sciences

  The University of Tokyo;Kyoto University

  齊藤 博英

  From 30 Jul. 2020, To 31 Mar. 2025, Granted

  RNA;人工細胞;合成生物学;合成生命システム;RNP;RNA-Protein相互作用;人工オルガネラ;生命の起源;artificial cell;synthetic biology

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H05626/
• マイクロRNAによる全能性・多能性制御機構の解明

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Biological Sciences

  Kyoto University

  齊藤 博英

  From 01 Apr. 2020, To 31 Mar. 2021, Discontinued

  マイクロRNA;転写後制御;分化

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-20H05363/
• Creation and development of programmable dynamic microparticles "auto-matter"

  Grant-in-Aid for Scientific Research (A)

  Medium-sized Section 61:Human informatics and related fields

  Tohoku University

  野村 慎一郎

  From 01 Apr. 2020, To 31 Mar. 2021, Discontinued

  人工多細胞;人工細胞;分子ロボティクス;オートマター;自己複製;大量生産;電子-分子インタフェイス

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H00598/
• Construction of artificial gene circuit and cell programming based on RNA network control

  Grant-in-Aid for Scientific Research (A)

  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields

  Kyoto University

  齊藤 博英

  From 01 Apr. 2020, To 31 Mar. 2021, Discontinued

  合成生物学;細胞運命制御;遺伝子回路;マイクロRNA;転写後制御

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H00452/
• 大規模RNA構造ライブラリを活用した真のRNA高次構造エレメントの解明

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields

  Kyoto University

  齊藤 博英

  From 28 Jun. 2019, To 31 Mar. 2021, Discontinued

  RNA高次構造;RNA structurome;RNA G-quadruplex;Hoogsten 塩基対;RNAモチーフ;RNA;RNP;RNA結合リガンド;RNA構造;RNA Structurome;G-quadruplex

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K22387/
• Elucidation of mechanism underlying the chondrogenesis-specific translational regulation

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields

  Kyoto University

  YONGHUI JIN

  From 29 Jun. 2018, To 31 Mar. 2020, Project Closed

  軟骨分化;翻訳制御;iPS細胞;分化段階特異的;翻訳制御機構;軟骨分化誘導;RIPアッセイ

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K19614/
• ncRNA構造ライブラリを基盤とする大規模作動エレメント解析技術の開発

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Biological Sciences

  Kyoto University

  齊藤 博英

  From 01 Apr. 2017, To 31 Mar. 2019, Granted

  機能性RNA;リガンド結合性RNA;大規模機能解析技術;RNA高次構造;RNAモチーフ

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-17H05601/
• 人工ＲＮＡを活用した高効率・汎用的な細胞リプログラミング技術の確立

  Grant-in-Aid for JSPS Fellows

  Kyoto University

  齊藤 博英

  From 10 Nov. 2017, To 31 Mar. 2020, Project Closed

  RNA methylation;microRNA;iPSC differentiation

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17F17390/
• Cellular Programming Using Synthetic RNP Nanosystems

  Grant-in-Aid for Scientific Research (S)

  Kyoto University

  Hirohide SAITO

  From 29 May 2015, To 31 Mar. 2020, Project Closed

  合成生物学;生体機能発現;RNA;RNP;人工遺伝子回路;細胞制御;生体分子;再生医学;進化分子工学;ナノバイオ;核酸;幹細胞;再生医療;遺伝子回路;生体内発現;分化;発生;ナノテクノロジー;マイクロRNA;iPS細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H05722/
• Developing DNA / RNA molecular devices for constructing a new generation of artificial cells.

  Grant-in-Aid for Scientific Research (B)

  Tohoku University

  Shin-ichiro NOMURA

  From 01 Apr. 2015, To 31 Mar. 2018, Project Closed

  人工細胞;分子ロボティクス;RNAナノテクノロジー;DNAナノテクノロジー;DNAコンピュータ;細胞融合;RNP;バイオテクノロジー

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H02774/
• マイクロRNAスイッチを活用した標的細胞の識別及び運命制御

  Grant-in-Aid for Scientific Research (A)

  Kyoto University

  齊藤 博英

  From 01 Apr. 2015, To 31 Mar. 2016, Discontinued

  マイクロRNA;RNAスイッチ;細胞選別;iPS細胞;分化;再生医療;合成生物学;翻訳

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H02376/
• Identification and separation of living cells based on synthetic RNA circuits detecting intracellular information

  Grant-in-Aid for Young Scientists (B)

  The University of Tokyo;Kyoto University

  Kei ENDO

  From 01 Apr. 2013, To 31 Mar. 2016, Project Closed

  細胞分画法;mRNA;マイクロRNA;人口遺伝子回路;合成生物学;再生医療;miRNA;遺伝子スイッチ;細胞分画;遺伝子回路;細胞識別;microRNA

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25870355/
• Construction of multi-molecular information conversion devices by employing nucleic acid nanostructures

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Science and Engineering

  Kyoto University

  Saito Hirohide

  From 28 Jun. 2012, To 31 Mar. 2017, Project Closed

  分子ロボティクス;DNAナノ構造;RNA/RNPナノ構造;分子センサー・人工レセプター;リポソーム;人工細胞モデル;自律性;少数分子化学反応;RNA;DNAナノ構造体;人工レセプター;センサー;核酸ナノ構造;核酸スイッチ;情報交換デバイス

  https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-24104002/
• Support and publicity of molecular robotics

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Science and Engineering

  The University of Tokyo

  Masami Hagiya

  From 28 Jun. 2012, To 31 Mar. 2017, Project Closed

  分子ロボティクス;ナノマシン;ナノバイオ;DNAコンピュータ;知能ロボティクス

  https://kaken.nii.ac.jp/grant/KAKENHI-ORGANIZER-24104001/
• Controlling cell function by synthetic biology approaches

  Grant-in-Aid for Young Scientists (A)

  Kyoto University

  Hirohide SAITO

  From 01 Apr. 2011, To 31 Mar. 2014, Project Closed

  合成生物学;RNA;翻訳;RNP;バイオエンジニアリング;遺伝子回路;細胞分化;遺伝子スイッチ;遺伝子発現制御

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23681042/
• Synthetic approaches to investigate artificial living systems by RNA self-replicating ribozymes

  Grant-in-Aid for Challenging Exploratory Research

  Kyoto University

  Hirohide SAITO

  From 01 Apr. 2011, To 31 Mar. 2013, Project Closed

  RNA;人工細胞モデル;生命の起源;合成生物学;人工細胞;RNP;バイオエンジニアリング;リポソーム;実験進化

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651203/
• Design and construction of new systems for regulating human cell fate

  Grant-in-Aid for Scientific Research (S)

  Kyoto University

  Tan INOUE

  From 01 Apr. 2011, To 31 Mar. 2015, Project Closed

  RNP;シンセティックバイオロジー;バイオナノテクノロジー

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23221011/
• Evolutionary engineering of biomacromolecules for improvement of artificial genetic circuits

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Complex systems

  Tokyo Institute of Technology

  Daisuke Kiga

  From 01 Apr. 2011, To 31 Mar. 2016, Project Closed

  合成生物学;進化分子工学;タンパク質;RNA;数理モデル;リボザイム;遺伝暗号;ＲＮＡ;nanobio technology;信号伝達制御

  https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-23119005/
• RNAネットワークを内包した人工細胞の創成

  Grant-in-Aid for Exploratory Research

  Kyoto University

  齊藤 博英

  From 01 Apr. 2007, To 31 Mar. 2009, Project Closed

  人工細胞モデル;RNA;翻訳;リボスイッチ;シンセティックバイオロジー;リポソーム;RNP;情報変換素子;無細胞蛋白質合成;翻訳制御

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19651091/
• シンセティック・バイオロジーによる人工RNP複合体及びシステム進化系の構築

  Grant-in-Aid for Young Scientists (A)

  Kyoto University

  齊藤 博英

  From 01 Apr. 2006, To 31 Mar. 2009, Project Closed

  RNA;進化分子工学;翻訳;リボスイッチ;シンセティックバイオロジー;遺伝子回路;RNP;遺伝子発現制御;リボザイム;試験管内進化

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18681034/
• RNPによる人工遺伝子ネットワークの試験管内進化

  Grant-in-Aid for Scientific Research on Priority Areas

  Science and Engineering

  Kyoto University

  齊藤 博英

  From 01 Apr. 2006, To 31 Mar. 2009, Project Closed

  RNP;試験管内進化;合成生物学;ナノバイオロジー;分子設計;生命の起源;RNA;翻訳;シンセティックバイオロジー;人工蛋白質;進化分子工学;人工細胞

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18048027/
• 特殊RNA合成生物学を基盤とする生命システムの制御と創成

  Grant-in-Aid for Scientific Research (A)

  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields

  The University of Tokyo

  齊藤 博英

  From 01 Apr. 2025, To 31 Mar. 2028, Adopted

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25H00970/

External funds: others

• 新エネルギー・産業技術総合開発機構（NEDO) 産業技術研究助成事業

  From 2013, To 2013

  齊藤　博英
• ＲＮＡ構造を標的とした創薬基盤・ＦＯＲＥＳＴにおける解析スケールの拡張 （2020年度分）

  京都発革新的医療技術研究開発助成事業

  From 01 Apr. 2020, To 31 Mar. 2021

  齊藤　博英

Education

Teaching subject(s)

• From Apr. 2011, To Mar. 2012

  Synergetic Studies for Space

  Spring, 全学共通科目
• From Apr. 2012, To Mar. 2013

  Synergetic Studies for Space

  Spring, 全学共通科目
• From Apr. 2013, To Mar. 2014

  Synergetic Studies for Space

  Spring, 全学共通科目
• From Apr. 2013, To Mar. 2014

  In order to Know, Observe, and Create our Life : The Molecular Science for Life

  Spring, 全学共通科目
• From Apr. 2014, To Mar. 2015

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2014, To Mar. 2015

  Synergetic Studies for Space

  Spring, 全学共通科目
• From Apr. 2014, To Mar. 2015

  Life Sciences: Knowledge, Observation, and Creation of a Life

  Spring, 全学共通科目
• From Apr. 2015, To Mar. 2016

  Synergetic Studies for Space

  Spring, 全学共通科目
• From Apr. 2015, To Mar. 2016

  Biocyclology: interface between philosophy and science

  Spring, 全学共通科目
• From Apr. 2015, To Mar. 2016

  Understanding life: From molecule to ecosystem.

  Spring, 全学共通科目
• From Apr. 2016, To Mar. 2017

  Global Frontier in Life Science A

  Spring, 生命科学研究科
• From Apr. 2017, To Mar. 2018

  Basic Introduction in Medical Science

  Spring, 医学研究科

Participation in PhD Defense

• PDIVAS: Pathogenicity predictor for Deep-Intronic Variants causing Aberrant Splicing

  KUROSAWA RYO, Graduate School of Medicine, Sub-chief Examiner

  24 Mar. 2025
• miR-124-3p downregulates EGR1 to suppress ischemia-hypoxia reperfusion injury in human iPS cell-derived cardiomyocytes

  YANG QIAOKE, Graduate School of Medicine, Sub-chief Examiner

  23 Jan. 2025
• Oxidative phosphorylation is a pivotal therapeutic target of fibrodysplasia ossificans progressiva

  SUN LIPING, Graduate School of Medicine, Chief Examiner

  23 May 2024
• Chemoproteomic Identification of Blue-Light-Damaged Proteins

  TOH KOHEI, Graduate School of Medicine, Chief Examiner

  25 Mar. 2024
• Branchpoints as potential targets of exon-skipping therapies for genetic disorders

  OHARA HIROAKI, Graduate School of Medicine, Chief Examiner

  23 Jan. 2024
• SMN promotes mitochondrial metabolic maturation during myogenesis by regulating the MYOD-miRNA axis

  UEHARA AKIHIRO, Graduate School of Medicine, Chief Examiner

  24 Jul. 2023
• Inhibition of microRNA-33b specifically ameliorates abdominal aortic aneurysm formation via suppression of inflammatory pathways

  YAMASAKI TOMOHIRO, Graduate School of Medicine, Sub-chief Examiner

  23 Mar. 2023
• An ancient retroviral RNA element hidden in mammalian genomes and its involvement in co-opted retroviral gene regulation

  KITAO KOICHI, Graduate School of Medicine, Chief Examiner

  23 Mar. 2023
• The Borna disease virus (BoDV) 2 nucleoprotein is a conspecific protein that enhances BoDV-1 RNA-dependent RNA polymerase activity

  KANDA TAKEHIRO, Graduate School of Medicine, Sub-chief Examiner

  23 Mar. 2022
• Multicellular Modeling of Ciliopathy by Combining iPS cells and Microfluidic Airway-on-a-chip Technology

  SONE NAOYUKI, Graduate School of Medicine, Chief Examiner

  24 Nov. 2021
• Extracellular vesicles synchronize cellular phenotypes of differentiating cells

  MINAKAWA TOMOHIRO, Graduate School of Medicine, Chief Examiner

  23 Mar. 2022
• Local application of Usag-1 siRNA can promote tooth regeneration in Runx2-deficient mice

  MISHIMA SAYAKA, Graduate School of Medicine, Sub-chief Examiner

  24 Jan. 2022

Administration

School management (title, position)

• From 01 Apr. 2017, To 31 Mar. 2018

  iＰＳ細胞学系長
• From 01 Apr. 2017, To 31 Mar. 2020

  iＰＳ細胞学系長
• From 01 Apr. 2017, To 31 Mar. 2022

  iＰＳ細胞学系 学系長

Faculty management (title, position)

• From 01 Apr. 2025, To 31 Mar. 2027

  医学図書館運営委員会
• From 01 Apr. 2024, To 31 Mar. 2026

  白眉センター専門委員会
• From 01 Oct. 2023, To 31 Mar. 2026

  人権委員会1号委員
• From 01 Apr. 2022, To 31 Mar. 2026

  財務委員会
• From 01 Apr. 2017, To 31 Mar. 2020

  副所長
• To 31 Mar. 2022

  管理運営委員会2号委員
• To 31 Mar. 2020

  利益相反審査委員会2号委員
• 教員表彰選考委員会2号委員
• ERES/CSV委員会
• From 01 Apr. 2020, To 31 Mar. 2024

  未来生命科学開拓部門長
• From 06 Jun. 2019, To 31 Mar. 2024

  自己点検・評価委員会委員長、1号委員