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Shinohara, Takashi
Graduate School of Medicine, Medicine Professor
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Last Updated :2025/05/02
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Last Updated :2025/05/02
Research
Research Topics, Overview of the research
Research Topics
Genetic modification of the germline cells, Culture of germline cells, In vitro spermatogenesisOverview of the research
Our long-term goal is to use spermatogonial stem cells for genetic modification. We have recently succeeded in long-term culture of mouse spermatogonial stem cells. Due to their unique morphology, we named them germline stem (GS) cells. GS cells have several advantages over ES cells. First, although ES cells are only available during the embryonic period, GS cells can be derived from postnatal animals. Second, they are not tumorigenic and committed to the germline lineage. Given that ES cells with germline potential have been obtained only from mice, our GS cell technology may resolve current challenges with ES cells and greatly contribute to the development of new transgenic technologies. We are now trying 1) to modify the genome of GS cells and 2) to derive GS cells from other animal species.
Research Areas
Papers
- Restoration of fertility in nonablated recipient mice after spermatogonial stem cell transplantation.Hiroko Morimoto; Narumi Ogonuki; Shogo Matoba; Mito Kanatsu-Shinohara; Atsuo Ogura; Takashi ShinoharaStem cell reports, 09 Apr. 2024
- Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cellsHiroko Morimoto; Mito Kanatsu-Shinohara; Kyle E. Orwig; Takashi ShinoharaBiology of Reproduction, 12 Feb. 2020
- Lower developmental potential of rat zygotes produced by ooplasmic injection of testicular spermatozoa versus cauda epididymal spermatozoaMisuzu IDE; Ibuki SAITO; Makoto SANBO; Mito KANATSU-SHINOHARA; Takashi SHINOHARA; Masumi HIRABAYASHI; Shinichi HOCHIJournal of Reproduction and Development, 2024
- Allogeneic offspring produced by induction of PD-L1 in spermatogonial stem cells via self-renewal stimulation.Takashi Shinohara; Takuya Yamamoto; Hiroko Morimoto; Yusuke Shiromoto; Mito Kanatsu-ShinoharaStem cell reports, 11 Apr. 2023
- Glutamine protects mouse spermatogonial stem cells against NOX1-derived ROS for sustaining self-renewal division in vitro.Takehiro Miyazaki; Mito Kanatsu-Shinohara; Narumi Ogonuki; Shogo Matoba; Atsuo Ogura; Chihiro Yabe-Nishimura; Hongliang Zhang; Yves Pommier; Andreas Trumpp; Takashi ShinoharaDevelopment (Cambridge, England), 08 Mar. 2023
- Signal regulatory protein alpha is a conserved marker for mouse and rat spermatogonial stem cells.Takehiro Miyazaki; Mito Kanatsu-Shinohara; Masatsugu Ema; Takashi ShinoharaBiology of reproduction, 16 Jan. 2023
- Adenovirus-mediated gene delivery restores fertility in congenitally infertile female mice.Mito Kanatsu-Shinohara; Jiyoung Lee; Takehiro Miyazaki; Hiroko Morimoto; Takashi ShinoharaThe Journal of reproduction and development, 19 Dec. 2022
- Regulation of male germline transmission patterns by the Trp53-Cdkn1a pathway.Mito Kanatsu-Shinohara; Honda Naoki; Takashi Tanaka; Misako Tatehana; Takako Kikkawa; Noriko Osumi; Takashi ShinoharaStem cell reports, 01 Aug. 2022
- Adeno-associated-virus-mediated gene delivery to ovaries restores fertility in congenital infertile mice.Mito Kanatsu-Shinohara; Jiyoung Lee; Takehiro Miyazaki; Hiroko Morimoto; Takashi ShinoharaCell reports. Medicine, 17 May 2022
- 精子幹細胞のホーミングにおける分子機構(Molecular mechanisms of spermatogonial stem cell homing to niche)篠原 美都; 高島 誠司; 篠原 隆司日本生化学会大会プログラム・講演要旨集, Sep. 2011
- Regeneration of spermatogenesis by mouse germ cell transplantation into allogeneic and xenogeneic testis primordia or organoids.Mito Kanatsu-Shinohara; Narumi Ogonuki; Shogo Matoba; Hiroko Morimoto; Yusuke Shiromoto; Atsuo Ogura; Takashi ShinoharaStem cell reports, 12 Apr. 2022
- Genomic stability of mouse spermatogonial stem cells in vitroShinichiro Chuma; Mito Kanatsu-Shinohara; Ami Katanaya; Mihoko Hosokawa; Takashi ShinoharaScientific Reports, Dec. 2021
- Cdc42 is required for male germline niche development in mice.Yoshifumi Mori; Seiji Takashima; Mito Kanatsu-Shinohara; Zheng Yi; Takashi ShinoharaCell reports, 17 Aug. 2021
- Spermatogonial stem cell transplantation into nonablated mouse recipient testes.Hiroko Morimoto; Narumi Ogonuki; Mito Kanatsu-Shinohara; Shogo Matoba; Atsuo Ogura; Takashi ShinoharaStem cell reports, 13 Jul. 2021
- OGG1 protects mouse spermatogonial stem cells from reactive oxygen species in culture†.Yoshifumi Mori; Narumi Ogonuki; Ayumi Hasegawa; Mito Kanatsu-Shinohara; Atsuo Ogura; Yufeng Wang; John R McCarrey; Takashi ShinoharaBiology of reproduction, 11 Mar. 2021
- An interplay of NOX1-derived ROS and oxygen determines the spermatogonial stem cell self-renewal efficiency under hypoxia.Hiroko Morimoto; Takuya Yamamoto; Takehiro Miyazaki; Narumi Ogonuki; Atsuo Ogura; Takashi Tanaka; Mito Kanatsu-Shinohara; Chihiro Yabe-Nishimura; Hongliang Zhang; Yves Pommier; Andreas Trumpp; Takashi ShinoharaGenes & development, 01 Feb. 2021
- CD2 is a surface marker for mouse and rat spermatogonial stem cells.Mito Kanatsu-Shinohara; Guiying Chen; Hiroko Morimoto; Takashi ShinoharaThe Journal of reproduction and development, 20 Aug. 2020, Peer-reviewed
- Autologous transplantation of spermatogonial stem cells restores fertility in congenitally infertile mice.Mito Kanatsu-Shinohara; Narumi Ogonuki; Shogo Matoba; Atsuo Ogura; Takashi ShinoharaProceedings of the National Academy of Sciences of the United States of America, 07 Apr. 2020, Peer-reviewed
- Transgenesis and Genome Editing of Mouse Spermatogonial Stem Cells by Lentivirus Pseudotyped with Sendai Virus F Protein.Takashi Shinohara; Mito Kanatsu-ShinoharaStem cell reports, 10 Mar. 2020, Peer-reviewed
- Aging of spermatogonial stem cells by Jnk-mediated glycolysis activation.Mito Kanatsu-Shinohara; Takuya Yamamoto; Hidehiro Toh; Yasuhiro Kazuki; Kanako Kazuki; Junichi Imoto; Kazuho Ikeo; Motohiko Oshima; Katsuhiko Shirahige; Atsushi Iwama; Yoichi Nabeshima; Hiroyuki Sasaki; Takashi ShinoharaProceedings of the National Academy of Sciences of the United States of America, 13 Aug. 2019, Peer-reviewed
- ROS amplification drives mouse spermatogonial stem cell self-renewal.Hiroko Morimoto; Mito Kanastu-Shinohara; Narumi Ogonuki; Satoshi Kamimura; Atsuo Ogura; Chihiro Yabe-Nishimura; Yoshifumi Mori; Takeshi Morimoto; Satoshi Watanabe; Kinya Otsu; Takuya Yamamoto; Takashi ShinoharaLife science alliance, Apr. 2019, Peer-reviewed
- Sendai virus-mediated transduction of mammalian spermatogonial stem cells.Watanabe, S; Kanatsu-Shinohara, M; Shinohara, TBiol. Reprod., 2019, Peer-reviewed
- Culture and transplantation of spermatogonial stem cells.Takashima, S; Shinohara, TStem Cell Res., 2018, Peer-reviewed
- In vivo genetic manipulation of male germline stem cells and their microenvironment by adeno-associated viruses.Watanabe, S; Kanatsu-Shinohara, M; Ogonuki, N; Matoba, S; Ogura, A; Shinohara, TStem Cell Reports, 2018, Peer-reviewed
- Reversible inhibition of the blood-testis barrier protein improves stem cell homing in mouse testes.Kanatsu-Shinohara, M; Morimoto, H; Watanabe, S; Shinohara, TJ. Reprod. Dev., 2018, Peer-reviewed
- Transfer of a Mouse Artificial Chromosome into Spermatogonial Stem Cells Generates Transchromosomic MiceTakashi Shinohara; Kanako Kazuki; Narumi Ogonuki; Hiroko Morimoto; Shogo Matoba; Kei Hiramatsu; Kazuhisa Honma; Teruhiko Suzuki; Takahiko Hara; Atsuo Ogura; Mitsuo Oshimura; Mito Kanatsu-Shinohara; Yasuhiro KazukiSTEM CELL REPORTS, Oct. 2017, Peer-reviewed
- Adeno-associated virus-mediated delivery of genes to mouse spermatogonial stem cellsSatoshi Watanabe; Mito Kanatsu-Shinohara; Narumi Ogonuki; Shogo Matoba; Atsuo Ogura; Takashi ShinoharaBIOLOGY OF REPRODUCTION, Jan. 2017, Peer-reviewed
- Nonrandom contribution of left and right testes to germline transmission from mouse spermatogonial stem cells.Kanatsu-Shinohara, M; Naoki, H; Shinohara, TBiol. Reprod., 2017, Peer-reviewed
- Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewalMito Kanatsu-Shinohara; Takashi Tanaka; Narumi Ogonuki; Atsuo Ogura; Hiroko Morimoto; Pei Feng Cheng; Robert N. Eisenman; Andreas Trumpp; Takashi ShinoharaGENES & DEVELOPMENT, Dec. 2016, Peer-reviewed
- Nonrandom Germline Transmission of Mouse spermatogonial Stem CellsMito Kanatsu-Shinohara; Honda Naoki; Takashi ShinoharaDEVELOPMENTAL CELL, Aug. 2016, Peer-reviewed
- The Luteinizing Hormone-Testosterone Pathway Regulates Mouse Spermatogonial Stem Cell Self-Renewal by Suppressing WNT5A Expression in Sertoli CellsTakashi Tanaka; Mito Kanatsu-Shinohara; Zhenmin Lei; C. V. Rao; Takashi ShinoharaSTEM CELL REPORTS, Aug. 2016, Peer-reviewed
- Fertility of Male Germline Stem Cells Following Spermatogonial Transplantation in Infertile Mouse ModelsMito Kanatsu-Shinohara; Hiroko Morimoto; Takashi ShinoharaBIOLOGY OF REPRODUCTION, May 2016, Peer-reviewed
- Enrichment of Mouse Spermatogonial Stem Cells by the Stem Cell Dye CDy1Mito Kanatsu-Shinohara; Hiroko Morimoto; Takashi ShinoharaBIOLOGY OF REPRODUCTION, Jan. 2016, Peer-reviewed
- Pluripotent cell derivation from male germline cells by suppression of Dmrt1 and Trp53Takashi Tanaka; Mito Kanatsu-Shinohara; Michiko Hirose; Atsuo Ogura; Takashi ShinoharaJOURNAL OF REPRODUCTION AND DEVELOPMENT, Oct. 2015, Peer-reviewed
- The CDKN1B-RB1-E2F1 pathway protects mouse spermatogonial stem cells from genomic damageTakashi Tanaka; Mito Kanatsu-Shinohara; Takashi ShinoharaJOURNAL OF REPRODUCTION AND DEVELOPMENT, Aug. 2015, Peer-reviewed
- ROS-Generating Oxidase Nox3 Regulates the Self-Renewal of Mouse Spermatogonial Stem CellsHiroko Morimoto; Mito Kanatsu-Shinohara; Takashi ShinoharaBIOLOGY OF REPRODUCTION, Jun. 2015, Peer-reviewed
- Functional Differences between GDNF-Dependent and FGF2-Dependent Mouse Spermatogonial Stem Cell Self-RenewalSeiji Takashima; Mito Kanatsu-Shinohara; Takashi Tanaka; Hiroko Morimoto; Kimiko Inoue; Narumi Ogonuki; Mayumi Jijiwa; Masahide Takahashi; Atsuo Ogura; Takashi ShinoharaSTEM CELL REPORTS, Mar. 2015, Peer-reviewed
- Induction of Primordial Germ Cell-Like Cells From Mouse Embryonic Stem Cells by ERK Signal InhibitionTohru Kimura; Yoshiaki Kaga; Hiroshi Ohta; Mika Odamoto; Yoichi Sekita; Kunpeng Li; Noriko Yamano; Keita Fujikawa; Ayako Isotani; Norihiko Sasaki; Masashi Toyoda; Katsuhiko Hayashi; Masaru Okabe; Takashi Shinohara; Mitinori Saitou; Toru NakanoSTEM CELLS, Oct. 2014, Peer-reviewed
- Improved Serum- and Feeder-Free Culture of Mouse Germline Stem CellsMito Kanatsu-Shinohara; Narumi Ogonuki; Shogo Matoba; Hiroko Morimoto; Atsuo Ogura; Takashi ShinoharaBIOLOGY OF REPRODUCTION, Oct. 2014, Peer-reviewed
- The Trp53-Trp53inp1-Tnfrsf10b Pathway Regulates the Radiation Response of Mouse Spermatogonial Stem CellsKei Ishii; Masamichi Ishiai; Hiroko Morimoto; Mito Kanatsu-Shinohara; Ohtsura Niwa; Minoru Takata; Takashi ShinoharaSTEM CELL REPORTS, Oct. 2014, Peer-reviewed
- Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewalMito Kanatsu-Shinohara; Ichiro Onoyama; Keiichi I. Nakayama; Takashi ShinoharaPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jun. 2014, Peer-reviewed
- Cell-cycle-dependent Colonization of Mouse Spermatogonial Stem Cells After Transplantation into Seminiferous TubulesKei Ishii; Mito Kanatsu-Shinohara; Takashi ShinoharaJOURNAL OF REPRODUCTION AND DEVELOPMENT, Feb. 2014, Peer-reviewed
- Enrichment of mouse spermatogonial stem cells based on aldehyde dehydrogenase activityMito Kanatsu-Shinohara; Yoshifumi Mori; Takashi ShinoharaBiology of Reproduction, 2013, Peer-reviewed
- Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantationMito Kanatsu-Shinohara; Seiji Takashima; Takashi ShinoharaPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Apr. 2010, Peer-reviewed
- Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cellsArata Honda; Michiko Hirose; Kenshiro Hara; Shogo Matoba; Kimiko Inoue; Himomi Miki; Hitoshi Hiura; Mito Kanatsu-Shinohara; Yoshiakira Kanai; Tomohiro Kono; Takashi Shinohara; Atsuo OguraPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jul. 2007, Peer-reviewed
- Adenovirus-mediated gene delivery into mouse spermatogonial stem cellsMasanori Takehashi; Mito Kanatsu-Shinohara; Kimiko Inoue; Narumi Ogonuki; Hiromi Miki; Shinya Toyokuni; Atsuo Ogura; Takashi ShinoharaPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Feb. 2007, Peer-reviewed
- Clonal origin of germ cell colonies after spermatogonial transplantation in miceM Kanatsu-Shinohara; K Inoue; H Miki; N Ogonuki; M Takehashi; T Morimoto; A Ogura; T ShinoharaBIOLOGY OF REPRODUCTION, Jul. 2006, Peer-reviewed
- The use of chromosome-based vectors for animal transgenesisY. Kuroiwa; H. Yoshida; T. Ohshima; T. Shinohara; A. Ohguma; Y. Kazuki; M. Oshimura; I. Ishida; K. TomizukaGene Therapy, 2002
Misc.
- 「万病」を癒すスーパー幹細胞--精原幹細胞のバイオロジー (特集 新世紀医療をめざして(2)クローンと再生医学--Cell therapyに向かう医学のフロンティア)篠原 隆司; 篠原 美都遺伝子医学, May 2000
- 再生医学と精子幹細胞:"次世代"幹細胞の新たな可能性と危険性 (再生医学・再生医療) -- (第1部 再生医学における幹細胞の意義と可能性)篠原 隆司; 篠原 美都現代化学増刊, Jul. 2002
- Pluripotency of spermatogonial stem cells and their potential in medical applications篠原 美都; 篠原 隆司最新医学, Mar. 2009
- 再生医学のいま--基礎研究から臨床への展開に向けて(9)精子幹細胞研究を用いたノックアウト動物の作成とその意外な展開篠原 隆司; 篠原 美都治療, Jul. 2007
- Spermatogonial stem cells: utility in the production of gene knockout and their unexpected features篠原 美都; 篠原 隆司Regenerative medicine, Aug. 2007
- 精子幹細胞の機能的寿命の維持機構篠原 美都; 本田 直樹; 篠原 隆司実験医学, May 2017, Invited
- Aging of Male Germline Stem Cells篠原 美都; 篠原 隆司Anti-aging medicine, Feb. 2013
- Transcriptional and epigenetic landscape of mouse germline stem cellsMihoko Hosokawa; Ami Katanaya; Eri Hayashi; Ayako Mochizuki; Hirofami Suemori; Koji Numata; Kuniya Abe; Takashi Tanaka; Mito Kanatsu-Shinohara; Takashi Shinohara; Norio Nakatsuji; Shinichiro ChumaGENES & GENETIC SYSTEMS, Dec. 2012
- マウス生殖幹細胞のゲノムーエピゲノム解析細川美穂子; 刀谷在美; 林瑛理; 望月綾子; 末盛博文; 沼田興治; 阿部訓也; 田中敬; 篠原美都; 篠原隆司; 中辻憲夫; 中馬新一郎日本遺伝学会第84 回大会（2012.9.26， 福岡）, 2012, Peer-reviewed
- マウス生殖幹細胞の体細胞型増殖から第一減数分裂前期への移行細川美穂子; 田中敬; 林瑛理; 篠原美都; 篠原隆司; 中辻憲夫; 中馬新一郎特定領域研究生殖サイクル第4 回公開シンポジウム（11.17-18, 2011, 大阪）, 2011, Peer-reviewed
- Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantationMito Kanatsu-Shinohara; Seiji Takashima; Takashi ShinoharaPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Apr. 2010, Peer-reviewed
- マウス生殖幹細胞の減数分裂移行実験系作出の試み細川美穂子; 田中敬; 林瑛理; 篠原美都; 篠原隆司; 中辻憲夫; 中馬新一郎特定領域研究「生殖サイクル若手勉強会2010」（2010. 7. 21－23. Tsukuba）, 2010, Peer-reviewed
- Production of Transgenic Rats via Lentiviral Transduction and Xenogeneic Transplantation of Spermatogonial Stem Cells Editorial CommentM. Kanatsu-Shinohara; M. Kato; M. Takehashi; H. Morimoto; S. Takashima; S. Chuma; N. Nakatsuji; M. Hirabayashi; T. ShinoharaJOURNAL OF UROLOGY, Jul. 2009
- HERITABLE IMPRINTING DEFECT CAUSED BY FETAL MALE GERM CELL CULTURE IN MICETakashi Shinohara; Mito Kanatsu-Shinohara; Jiyoung LeeJOURNAL OF PHYSIOLOGICAL SCIENCES, 2009
- 精子幹細胞と再生医療竹橋正則; 篠原美都; 篠原隆司産科と婦人科, 2009
- Germline modification in spermatogonial stem cellsTakashi ShinoharaBIOLOGY OF REPRODUCTION, 2008
- 精子幹細胞による新しい遺伝子改変ストラテジー竹橋正則; 篠原美都; 篠原隆司実験医学, 2008
- トピックス：精子幹細胞の培養と遺伝子改変竹橋正則; 篠原美都; 篠原隆司最新医学, 2008
- Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cellsArata Honda; Michiko Hirose; Kenshiro Hara; Shogo Matoba; Kimiko Inoue; Himomi Miki; Hitoshi Hiura; Mito Kanatsu-Shinohara; Yoshiakira Kanai; Tomohiro Kono; Takashi Shinohara; Atsuo OguraPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jul. 2007, Peer-reviewed
- Adenovirus-mediated gene delivery into mouse spermatogonial stem cellsMasanori Takehashi; Mito Kanatsu-Shinohara; Kimiko Inoue; Narumi Ogonuki; Hiromi Miki; Shinya Toyokuni; Atsuo Ogura; Takashi ShinoharaPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Feb. 2007, Peer-reviewed
- Rats produced by interspecies spermatogonial transplantation in mice and in vitro microinseminationTakashi Shinohara; Megumi Kato; Masanori Takehashi; Jiyoung Lee; Shinichiro Chuma; Norio Nakatsuji; Mito Kanatsu-Shinohara; Masumi HirabayashiPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Sep. 2006, Peer-reviewed
- Functional Restoration of a Genetic Defect in Multipotent Germline Stem Cells by Human Artificial Chromosome Containing a Genomic InsertYasuhiro Kazuki; Hidetoshi Hoshiya; Yoshiteru Kai; Satoshi Abe; Masato Takiguchi; Mitsuhiko Osaki; Mito Kanatsu-Shinohara; Naoyo Kajitani; Yasuaki Shirayoshi; Masaharu Hiratuka; Astuo Ogura; Takashi Shinohara; Mitsuo OshimuraMOLECULAR THERAPY, May 2006
- Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cellsM Kanatsu-Shinohara; M Ikawa; M Takehashi; N Ogonuki; H Miki; K Inoue; Y Kazuki; J Lee; S Toyokuni; M Oshimura; A Ogura; T ShinoharaPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, May 2006, Peer-reviewed
- Culture and genetic modification of male mouse germline stem cells.Takashi Shinohara; Mito Kanatsu-ShinoharaBIOLOGY OF REPRODUCTION, 2006
- 用語解説：GS細胞竹橋正則; 篠原美都; 篠原隆司分子細胞治療, 2006
- Hematopoietic development of multipotent germline stem cells derived from neonatal mouse testis in vitro and in vivo.M Yoshimoto; T Heike; H Chang; S Baba; H Fujino; MK Shinohara; T Shinohara; T NakahataBLOOD, Nov. 2005
- Culture of mouse male germline stem cellsTakashi ShinoharaInternational Symposium on “Germ Cells, Epigenetics, Reprogramming and Embryonic Stem Cells” November 15-18, 2005 at Kyoto University, 2005, Peer-reviewed
- 未分化ES細胞特異的に発現する遺伝子群ECATにおけるDNA メチル化状態のプロファイリング今村公紀; 篠原隆司; 中川誠人; 山中伸弥第28回日本分子生物学会年会（2005.12.9. 福岡）, 2005, Peer-reviewed
- マウス精子幹細胞長期培養系の確立：Germline stem（GS）細胞のもたらす新たな可能性篠原隆司特定領域研究「生殖細胞の発生プロセス・再プログラム化とエピジェネティクス」による公開シンポジウム（2004.2.13）（京大医）, 2004, Peer-reviewed
- Direct visualization of transplanted hematopoietic cell reconstitution in intact mouse organs indicates the presence of a niche (vol 31, pg 733, 2003)M Yoshimoto; T Shinohara; T Heike; M Shiota; M Kanatsu-Shinohara; T NakahataEXPERIMENTAL HEMATOLOGY, Nov. 2003
- Adenovirus-mediated gene delivery and in vitro microinsemination produce offspring from infertile male miceM Kanatsu-Shinohara; A Ogura; M Ikegawa; K Inoue; N Ogonuki; K Tashiro; S Toyokuni; T Honjo; T ShinoharaPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Feb. 2002, Peer-reviewed
- Remodeling of the postnatal mouse testis is accompanied by dramatic changes in stem cell number and niche accessibilityT Shinohara; KE Orwig; MR Avarbock; RL BrinsterPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, May 2001, Peer-reviewed
- Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cellsT Shinohara; KE Orwig; MR Avarbock; RL BrinsterPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Jul. 2000, Peer-reviewed
- beta(1)- and alpha(6)-integrin are surface markers on mouse spermatogonial stem cellsT Shinohara; MR Avarbock; RL BrinsterPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, May 1999, Peer-reviewed
External funds: Kakenhi
- Functional analysis and culture of human spermatogonial stem cellsGrant-in-Aid for Challenging Research (Exploratory)Medium-sized Section 48:Biomedical structure and function and related fieldsKyoto UniversityTakashi ShinoharaFrom 28 Jun. 2019, To 31 Mar. 2022, Project Closed精子形成;精子幹細胞;ヒト;幹細胞;培養
- Isolation and functional analysis of genes controlling totipotencyGrant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)Biological SciencesOsaka University伊川 正人From 28 Jun. 2019, To 31 Mar. 2024, Grantedゲノム編集;精子成熟;受精;卵活性化;全能性;不妊
- Grant-in-Aid for Scientific Research on Innovative Areas "Totipotency" steering groupGrant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)Biological SciencesInstitute of Physical and Chemical Research小倉 淳郎From 28 Jun. 2019, To 31 Mar. 2024, Granted全能性;ゲノム再プログラム化;核移植クローン;受精;胎盤
- Behavior analysis of offspring derived from spermatogonial stem cell transplantationGrant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)Complex systemsKyoto University篠原 隆司From 01 Apr. 2019, To 31 Mar. 2021, Project Closed精子幹細胞;行動解析;エピジェネティックス;行動;精子形成;幹細胞移植
- Molecular Analysis of Spermatogonial Stem Cell AgingGrant-in-Aid for Scientific Research (S)Broad Section IKyoto UniversityTakashi ShinoharaFrom 11 Jun. 2018, To 31 Mar. 2023, Project Closed精子形成;顕微授精;老化;幹細胞;精子幹細胞
- epigenome dynamics and regulation in germ cellsGrant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)Kyoto University篠原 隆司From 01 Apr. 2018, To 31 Mar. 2019, Project Closed生殖細胞;エピゲノム;エピノゲノム
- Functional analysis of suppressive histone modification in sex determinationGrant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)Kyoto University篠原 隆司From 01 Apr. 2018, To 31 Mar. 2020, Project ClosedEzh2;センダイウイルス;精子形成;性転換;エピジェネティックス;性決定;精巣;卵巣
- Support for international research in "epigenome dynamics and regulation in germ cells"Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)Biological SciencesKyoto UniversityShinohara TakashiFrom 06 Nov. 2015, To 31 Mar. 2018, Project Closedエピジェネティクス;精子形成;卵子形成;核移植;顕微受精;生殖細胞
- Genetic engineering of primordial germ cells for germline modificationGrant-in-Aid for Challenging Exploratory ResearchKyoto UniversityTakashi ShinoharaFrom 01 Apr. 2015, To 31 Mar. 2018, Project Closed始原生殖細胞;精子形成;卵子形成;Kit
- Analyses and regulation of germline epigenomeGrant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)From 28 Jun. 2013, To 31 Mar. 2018
- The role of ROS in spermatogonial stem cell self-renewalGrant-in-Aid for Scientific Research (B)Kyoto UniversityTakashi SHINOHARAFrom 01 Apr. 2013, To 31 Mar. 2016, Project Closed精子幹細胞;精子形成;活性酸素;幹細胞
- Analysis of epigenome stability and germ cell tumor formationGrant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)Biological SciencesKyoto UniversityTakashi ShinoharaFrom 28 Jun. 2013, To 31 Mar. 2018, Project Closed精子形成;がん;解糖系;精子幹細胞;生殖細胞
- Regulation of epigenome dynamics and regulation in germ cellsGrant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)Biological SciencesKyoto UniversityTakashi ShinoharaFrom 28 Jun. 2013, To 31 Mar. 2018, Project Closedエピジェネティクス;精子形成;卵子形成;核移植;エピゲノム;生殖細胞;エピジェネティックス;発生・分化
- Reciprocal conversion between germ cells and pluripotent stem cellsGrant-in-Aid for Scientific Research (B)Kitasato UniversityTohru KIMURAFrom 01 Apr. 2012, To 31 Mar. 2015, Project Closed生殖細胞;多能性;幹細胞;分化誘導;ES細胞;EG細胞;iPS細胞;シグナル;相互転換
- Development of a long-term culture system for functional Sertoli cells.Grant-in-Aid for Challenging Exploratory ResearchKyoto UniversityTakashi SHINOHARAFrom 01 Apr. 2011, To 31 Mar. 2014, Project Closed精子形成;セルトリ細胞;精子幹細胞;増殖;T antigen
- 精子形成の試験管内誘導Grant-in-Aid for Challenging Exploratory ResearchKyoto University篠原 隆司From 01 Apr. 2008, To 31 Mar. 2011, Project Closed遺伝学;発生・分化;幹細胞;精子形成;自己複製;移植;顕微授精;セルトリ細胞
- Analyses on the quality control mechanism of mouse spermatogonial stem cellsGrant-in-Aid for Scientific Research on Priority AreasBiological SciencesKyoto UniversityTakashi SHINOHARAFrom 01 Apr. 2008, To 31 Mar. 2013, Project Closed精子形成;幹細胞;移植;培養;エピジェネティックス;自己複製;生殖細胞;遺伝学;発生・分化
- The improvement of microinsemination techniques and the production of transgenic offspring with GS cell-derived spermatids in the ratsGrant-in-Aid for Scientific Research (B)National Institute for Physiological SciencesMasumi HIRABAYASHIFrom 01 Apr. 2007, To 31 Mar. 2010, Project Closedラット;蛍光タンパク質遺伝子;精子幹細胞;人為的活性化;円形精子細胞;胚性幹細胞;精細管移植;キメラ;トランスジェニック;FGFレセプターインヒビター;MEK活性化インヒビター;GSK3インヒビター;LIF;レンチウイルス;レトロウイルス;異種間移植;精子;第二減数分裂後期;イオノマイシン;体内受精;DNAメチル化
- Production of knockout animals by spermatogonial stem cellsGrant-in-Aid for Scientific Research (A)Kyoto UniversityTakashi SHINOHARAFrom 01 Apr. 2007, To 31 Mar. 2011, Project Closed幹細胞;生殖;遺伝学;発生・分化;精子形成;移植;自己複製;顕微授精;セルトリ細胞;ノックアウト;オクルジン
- マウスモデルにおける生殖細胞での遺伝子治療の可能性の検討Grant-in-Aid for Young Scientists (A)Kyoto University篠原 隆司From 01 Apr. 2004, To 31 Mar. 2007, Project Closed幹細胞;精子形成;移植;ゲノム;遺伝子;分化;バイオテクノロジー
- 哺乳類精子幹細胞長期培養系の確立Grant-in-Aid for Exploratory ResearchKyoto University篠原 隆司From 01 Apr. 2004, To 31 Mar. 2006, Project Closed遺伝子;ゲノム;幹細胞;分化;バイオテクノロジー;精子形成;移植
- Development of rat models for human epilepsy by methods of gene modificationGrant-in-Aid for Scientific Research (A)Kyoto UniversityTadao SERIKWAFrom 01 Apr. 2004, To 31 Mar. 2008, Project Closedラット;てんかん;ミュータジェネシス;エチルニトロソウレア;疾患モデル;クロラムブチル;てんかん遺伝子;遺伝子改変ラット, Rat;Epilepsy;Mutagenesis;Etyl nitrosourea;Disease model;Chlorambucil;Epilepsy gene;Gene-modified rat
- 脳機能解析に用いるノックアウトラットの開発Grant-in-Aid for Scientific Research on Priority AreasBiological SciencesNational Institute for Physiological Sciences;Okazaki National Research Institutes平林 真澄From 01 Apr. 2003, To 31 Mar. 2008, Project Closed核移植;クローンラット;円形精子細胞;生殖幹細胞;遺伝子トラップ;ノックアウトラット;ROSI;脱メチル化;インプリンティング;トランスジェニックラット;EGFP;精子形成;顕微授精;p34^
kinase;精原細胞;コンディショナルターゲッティング;Cre-loxP;dsRNAi法 - 精子幹細胞の増殖機構の解明とその発生工学への応用Grant-in-Aid for Scientific Research on Priority AreasBiological SciencesKyoto University篠原 隆司From 01 Apr. 2003, To 31 Mar. 2008, Project Closed精子形成;幹細胞;ノックアウト;オクルジン;アデノウイルス;移植;ゲノム;遺伝子;分化;バイオテクノロジー;不妊;培養;精子幹細胞;精子;生殖細胞
- 精子幹細胞ニッシェ移植法の開発Grant-in-Aid for Scientific Research on Priority AreasBiological SciencesKyoto University篠原 隆司From 01 Apr. 2003, To 31 Mar. 2005, Project Closed精子形成;幹細胞;移植;ニッシェ;不妊;セルトリ細胞;精子;生殖細胞
- 細胞移植による体性幹細胞と生殖細胞の互換性の解析Grant-in-Aid for Scientific Research on Priority AreasBiological SciencesKyoto University篠原 隆司From 01 Apr. 2002, To 31 Mar. 2004, Project Closed精子形成;幹細胞;不妊;移植;培養;精子幹細胞;精子;生殖細胞;精巣
- Development of mouse spermatogonial purification system and its application for fertility restoration in miceGrant-in-Aid for Scientific Research (C)KYOTO UNIVERSITYTakashi SHINOHARAFrom 01 Apr. 2001, To 31 Mar. 2003, Project Closed不妊症;精子形成;幹細胞;移植;精巣;不妊治療;精原幹細胞, spermatogenesis;stem cell;infertility
- Mechanism for autoimmune dilated cardiomyopathy in PD-1-deficient miceGrant-in-Aid for Scientific Research (B)KYOTO UNIVERSITYNagahiro MINATOFrom 01 Apr. 2001, To 31 Mar. 2003, Project ClosedPD-1;PD-L1;抑制性レセプター;自己免疫疾患;自己抗体;自己抗原;拡張型心筋症, PD-1;PD-L1;Negative receptors;Autojmmune diseases;Autoantibody;Antoantigen;Dilated cardiomyopathy
- Isolation of PD-1 ligands and their application for immunosuppressionGrant-in-Aid for Scientific Research (B)KYOTO UNIVERSITYTasuku HONJOFrom 01 Apr. 2000, To 31 Mar. 2002, Project Closed自己免疫疾患;PD-1;DP-L1;PD-L2;SHP-2;PD-L1, Autoimmunity;PD-1;PD-L1;PD-L2;SHP-2
- 自己免疫疾患制御因子PD-1受容体の分子生物学的抑制機構の解明Grant-in-Aid for Scientific Research on Priority Areas (A)Biological SciencesKyoto University篠原 隆司From 01 Apr. 2000, To 31 Mar. 2002, Project Closed自己免疫疾患;PD-1;PD-L1
- Analysis of immune privilege of testis and production of allogeneic offspringGrant-in-Aid for Challenging Research (Exploratory)Medium-sized Section 49:Pathology, infection/immunology, and related fieldsKyoto University篠原 隆司From 30 Jun. 2022, To 31 Mar. 2025, Granted精子形成;PD-L1;免疫寛容
- Analysis of transgenerational abnormalities after germ cell manipulationGrant-in-Aid for Scientific Research (A)Medium-sized Section 48:Biomedical structure and function and related fieldsKyoto University篠原 隆司From 01 Apr. 2023, To 31 Mar. 2026, Granted精子幹細胞;生殖細胞;精子形成
- Next-generation ART: Building a global network to create innovative technologies in m ammalian reproductive engineeringFund for the Promotion of Joint International Research (International Leading Research )Biological SciencesOsaka University林 克彦From 17 Nov. 2023, To 31 Mar. 2030, Granted生殖細胞;生殖補助技術
- Impact of mouse ICSI on agingGrant-in-Aid for Transformative Research Areas (A)Transformative Research Areas, Section (III)Kyoto University篠原 隆司From 01 Apr. 2024, To 31 Mar. 2026, Granted顕微授精
External funds: others
list
Last Updated :2025/05/02
Education
Teaching subject(s)
- From 01 Apr. 2024, To 31 Mar. 2025Embryology and Developmental BiologyE004, Fall, Graduate School of Medicine, 2
- From 01 Apr. 2024, To 31 Mar. 2025EmbryologyB004, Fall, Faculty of Medicine, 1.5
- From 01 Apr. 2023, To 31 Mar. 2024Embryology and Developmental BiologyE004, Fall, Graduate School of Medicine, 2
- From 01 Apr. 2023, To 31 Mar. 2024EmbryologyB004, Fall, Faculty of Medicine, 1.5
- From 01 Apr. 2022, To 31 Mar. 2023Embryology and Developmental BiologyE004, Fall, Graduate School of Medicine, 2
- From 01 Apr. 2022, To 31 Mar. 2023EmbryologyB004, Fall, Faculty of Medicine, 1.5
- From 01 Apr. 2022, To 31 Mar. 2023Embryology and Developmental BiologyM013, Fall, Graduate School of Medicine, 2
- From 01 Apr. 2022, To 31 Mar. 2023EmbryologyL040, Fall, Faculty of Medicine, 0
- From Apr. 2011, To Mar. 2012発生学Fall, 医学研究科
- From Apr. 2012, To Mar. 2013Embryology and Developmental BiologyFall, 医学研究科
- From Apr. 2013, To Mar. 2014Embryology and Developmental BiologyFall, 医学部
- From Apr. 2013, To Mar. 2014Embryology and Developmental BiologyFall, 医学研究科
- From Apr. 2014, To Mar. 2015EmbryologyFall, 医学部
- From Apr. 2014, To Mar. 2015Embryology and Developmental BiologyFall, 医学研究科
- From Apr. 2015, To Mar. 2016EmbryologyFall, 医学部
- From Apr. 2015, To Mar. 2016Embryology and Developmental BiologyFall, 医学研究科
- From Apr. 2016, To Mar. 2017EmbryologyFall, 医学部
- From Apr. 2016, To Mar. 2017Embryology and Developmental BiologyFall, 医学研究科
- From Apr. 2017, To Mar. 2018EmbryologyFall, 医学部
- From Apr. 2017, To Mar. 2018EmbryologyFall, 医学部
- From Apr. 2017, To Mar. 2018Embryology and Developmental BiologyFall, 医学研究科
- From Apr. 2018, To Mar. 2019EmbryologyFall, 医学部
- From Apr. 2018, To Mar. 2019EmbryologyFall, 医学部
- From Apr. 2018, To Mar. 2019Embryology and Developmental BiologyFall, 医学研究科
- From Apr. 2019, To Mar. 2020EmbryologyFall, 医学部
- From Apr. 2019, To Mar. 2020EmbryologyFall, 医学部
- From Apr. 2019, To Mar. 2020Embryology and Developmental BiologyFall, 医学研究科
- From Apr. 2020, To Mar. 2021EmbryologyFall, 医学部
- From Apr. 2020, To Mar. 2021EmbryologyFall, 医学部
- From Apr. 2020, To Mar. 2021Embryology and Developmental BiologyFall, 医学研究科
- From Apr. 2021, To Mar. 2022EmbryologyFall, 医学部
- From Apr. 2021, To Mar. 2022EmbryologyFall, 医学部
- From Apr. 2021, To Mar. 2022Embryology and Developmental BiologyFall, 医学研究科
Participation in PhD Defense
- Nucleome programming is required for the foundation of totipotency in mammalian germline developmentNAGANO MASAHIRO, Graduate School of Medicine, Sub-chief Examiner24 Jul. 2023
- Calcium sparks enhance the tissue fluidity within epithelial layers and promote apical extrusion of transformed cellsKUROMIYA KEISUKE, Graduate School of Medicine, Chief Examiner23 Mar. 2023
- Ex vivo reconstitution of fetal oocyte development in humans and cynomolgus monkeysMIZUTA KEN, Graduate School of Medicine, Chief Examiner23 Mar. 2023
- Tracing the evolutionary history of blood cells to the unicellular ancestor of animalsNAGAHATA YOSUKE, Graduate School of Medicine, Sub-chief Examiner23 Jan. 2023
- Figla promotes secondary follicle growth in mature miceOKUNOMIYA ASUKA, Graduate School of Medicine, Sub-chief Examiner24 Nov. 2021
- Clinical impact of detecting low-frequency variants in cell-free DNA on treatment of castration-resistant prostate cancerMIZUNO KEI, Graduate School of Medicine, Sub-chief Examiner23 Mar. 2022
- Rescue of recurrent deep intronic mutation underlying cell type-dependent quantitative NEMO deficiencyHONDA YOSHITAKA, Graduate School of Medicine, Sub-chief Examiner24 May 2021
- Capturing human trophoblast development with naive pluripotent stem cells in vitroIO SHINGO, Graduate School of Medicine, Sub-chief Examiner24 Nov. 2021
- Three-dimensional human placenta-like bud synthesized from induced pluripotent stem cellsSATO MAI, Graduate School of Medicine, Sub-chief Examiner23 Mar. 2022
list
Last Updated :2025/05/02
Administration
School management (title, position)
- 高等教育開発推進センター協議委員
- From 15 Dec. 2009, To 30 Sep. 2010総長室特命補佐
- From 01 Apr. 2011, To 31 Mar. 2013教育制度委員会 委員
- From 01 Apr. 2013, To 30 Sep. 2014京都大学動物実験委員会 委員
- From 01 Oct. 2012, To 30 Sep. 2014動物実験委員会 委員
- From 01 Apr. 2013, To 31 Mar. 2015動物実験施設長
- From 01 Apr. 2011, To 31 Mar. 2012教育制度委員会：博士課程教育リーディングプログラム学位検討ＷＧ 委員
- From 01 Apr. 2011, To 31 Mar. 2012教育制度委員会WG２ 委員
- From 01 Apr. 2012, To 31 Mar. 2013教育制度委員会WG１ 委員
- From 01 Apr. 2013, To 10 Oct. 2013動物実験委員会 外部評価導入ＷＧ 委員
Faculty management (title, position)
- From 01 Oct. 2022, To 30 Sep. 2024管理体制委員会委員
- １０年後委員会
- 教育体制委員長
- 教育センター長
- 学位審査担当委員
- 大学院コースオーガナイザー
- M.D.／Ph.D.コース運営委員会
- カリキュラム委員
- 実験動物委員
- From 01 Jun. 2010, To 30 Apr. 2011医学研究科附属医学教育推進センター長
- From 01 Apr. 2014, To 31 Mar. 2015人事連絡委員会
- From 01 Apr. 2013, To 31 Mar. 2015動物実験施設管理委員会
- From 01 Apr. 2017, To 31 Mar. 2021医学研究科・医学部 将来計画検討委員会
- 医学研究科・医学部 放射性同位元素等管理委員会
- From 01 Oct. 2012, To 30 Sep. 2018医学研究科寄附講座検討委員会
- From 01 Jan. 2012, To 31 Dec. 2013医学部教育研究支援基金（ＫＭＳ―ＦＵＮＤ）委員会
- From 01 Oct. 2017, To 30 Sep. 2021大学評価部局委員会
- From 01 Apr. 2010, To 31 Mar. 2015寄附講座運営委員会（免疫ゲノム医学）
- From 26 Jan. 2012バイオ・フロンティア・プラットフォーム運営委員会
- To 30 Sep. 2016医学研究科運営委員会
- 放射性同位元素等管理委員会
- From 01 Apr. 2016, To 31 Mar. 2020寄附講座運営委員会（免疫ゲノム講座）
- From 01 Dec. 2016, To 30 Nov. 2020京都大学分子生物実験研究棟管理運営委員会
- From 01 Apr. 2017, To 31 Mar. 2019入学者選抜方法検討委員会
- From 01 May 2017, To 30 Apr. 2021京都大学ウイルス・再生医科学研究所及びiPS細胞研究所合同医の倫理委員会専門小委員会
- From 01 Oct. 2018, To 30 Sep. 2020管理体制委員会
- From 01 Oct. 2018, To 30 Sep. 2020産学共同講座等検討委員会
- 放射線障害防止委員会
- From 01 Nov. 2018, To 31 Oct. 2020教授選考方針検討委員会（基礎系）
- From 01 Oct. 2020, To 30 Sep. 2022管理体制委員会委員
- From 01 May 2021, To 30 Apr. 2023京都大学ウイルス・再生医科学研究所医の倫理委員会専門小委員会委員
