DNA nanoreactor self-assembled fluorescent probe functional bio-macromolecular system
Overview of the research
In nature, multi-enzyme synthetic pathways regulate the cellular activities. Understanding the effect of spatial organization on the enzymatic activity in multi-enzyme systems is very important not only for revealing a principle that associates with the function of multi-enzyme system but also for constructing an effective material conversion system in vitro. However, only a few methods are available to systematically evaluate how spatial factors such as distance, orientation and ratio of enzymes influence the efficiency of the enzyme cascade.
Research Interests
ナノ集合体
ケミカルバイオロジー
蛍光性ナノプローブ
バイオイメージングプローブ
レシオメトリー
タンパク質
核酸ナノ構造体
酵素反応アッセイ
ネットワーク
自己集合
細胞内動態
細胞内酵素反応
酵素反応
リアルタイム解析
細胞内pHプローブ
癌低pH環境
蛍光プローブ
蛍光イメージング
ナノリアクター
癌低酸素環境
蛍光波長変化
細胞取り込み過程
標的特異的検出
蛍光強度変化
CPP
動物細胞
音増感剤
超音波
SCI
Research Areas
Life sciences, Biomaterials
Life sciences, Biomedical engineering
Nanotechnology/Materials, Molecular biochemistry
Nanotechnology/Materials, Biochemistry
Nanotechnology/Materials, Nanobioscience
Nanotechnology/Materials, Nanomaterials
Papers
A Practical Approach for Polarity and Quantity Controlled Assembly of Membrane Proteins into Nanoliposomes
Journal of Inclusion Phenomena and Macrocyclic Chemistry, 20 Sep. 2021
Photophysical and elecron-transfer reaction properties of tris(2,2’-bipyridine)ruthenium(II)-based inhibitors that covalently bound to the active site of chymotrypsin
Medicinal Electronomics Bricolage Design of Hypoxia-targeting Antineoplastic Drugs and Invention of Boron Tracedrugs as Innovative Future-architectural Drugs
Hitoshi Hori; Yoshihiro Uto; Eiji Nakata
ANTICANCER RESEARCH, Sep. 2010, Peer-reviewed
Design of a SNARF-based Ratiometric Fluorescent Probe for Esterase
Semisynthetic Fluorescent Sensor Proteins Based on Self-Labeling Protein Tags
Matthias A. Brun; Kui-Thong Tan; Eiji Nakata; Marlon J. Hinner; Kai Johnsson
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Apr. 2009, Peer-reviewed
Constructuin of Tumor Implant Chick Embryo Model as a Next-generation Animal Model and the Evaluation of Tumor-growth Inhibitory Activity by x-ray Irradiation Using the Model
C. Abe; Y. Uto; Y. Endo; Y. Shinmoto; K. Nakashima; K Sano; Y. Sasaki; K. Minami; H. Maezawa; S. Masunaga; E. Nakata; H. Hori
Radiat Biol.Res.Commun、, 2009, Peer-reviewed
A CHEMICAL BIOSYNTHES IS DESIGN FOR AN ANTIATHEROSCLEROSIS DRUG BY ACYCLIC TOCOPHEROL INTERMEDIATE ANALOGUE BASED ON "ISOPRENOMICS"
One-pot and sequential organic chemistry on an enzyme surface to tether a fluorescent probe at the proximity of the active site with restoring enzyme activity
Y Takaoka; H Tsutsumi; N Kasagi; E Nakata; Hamachi, I
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Mar. 2006, Peer-reviewed
日本経済新聞, https://www.nikkei.com/article/DGXZQOUC16AD30W4A210C2000000/, Mar. 2024, Paper
「DNA折り紙」より頑強に
科学新聞, 科学新聞第3949号8面(裏表紙), Dec. 2023, Paper
DNA折り紙 安定化に成功 結合の切れ目 効率的に連結
京都大学新聞, 京都大学新聞第2709号 1面, Nov. 2023, Paper
「DNA折り紙」より頑強に、京大が新たな構造安定化法を開発
MIT Technology Review Japan, https://www.technologyreview.jp/n/2023/11/07/321500/?fbclid=IwAR1VZowKLypLFkBOT8RjAJE1fsV6avZMps7MVMfbfN2bFAcZKz_evet0XUg, Nov. 2023, Internet
External funds: Kakenhi
Construction of highly efficient enzyme reaction system by high-ordered self-assembly of DNA nanostructure