Basic Information

Professional Memberships

• From Oct. 2009, To Present

  Biophysical Society
• From Apr. 1993, To Present

  The Biophysical Society of Japan

Academic Degree

• M.Sc.(The University of Tokyo)
• D.Sc.(Nagoya University)

Academic Resume (Graduate Schools)

• The University of Tokyo, Graduate School of Arts and Sciences, Department of Life Sciences, 修了
• The University of Tokyo, Graduate School of Arts and Sciences, Department of Life Sciences, 単位修得退学

Academic Resume (Undergraduate School/Majors)

• Kyoto University, Faculty of Science, Department of Biophysics, 卒業

High School

• High School

  山口県立山口高等学校

Research History

• From Apr. 2017, To Present

  Kyoto University, Bioanalysis Unit, Analysis Center, Institute for Integrated Cell-Material Sciences, KUIAS, Unit Leader
• From Apr. 2017, To Present

  Kyoto University, KUIAS, Institute for Integrated Cell-Material Sciences, Program-Specific Associate Professor
• From May 2015, To Mar. 2017

  Kyoto University, Institute for Integrated Cell-Material Sciences, Program-Specific Research Center Associate Professor
• From Apr. 2013, To Mar. 2017

  Kyoto University, Center for Meso-Bio Single Molecule Imaging, Institute for Integrated Cell-Material Sciences, Deputy Director
• From Dec. 2010, To Apr. 2015

  Kyoto University, Institute for Integrated Cell-Material Sciences, Program-Specific Research Center Senior Lecturer
• From Apr. 2008, To Nov. 2010

  Kyoto University, Institute for Integrated Cell-Material Sciences, Research Assistant Professor
• From Apr. 2005, To Mar. 2010

  Kyoto University, Institute for Frontier Medical Sciences, Research Assistant Professor
• From Apr. 2005, To Mar. 2010

  ICORP, JST, Research Fellow
• From Oct. 2003, To Mar. 2005

  SORST, JST, Research Fellow
• From Oct. 1998, To Sep. 2003

  ERATO, JST, Research Fellow

Profile

• Profile

  I have been making efforts to develop single-molecule imaging techniques in living cells, and to use the methods for understanding the functional mechanisms of the cellular plasma membrane. 

Language of Instruction

• English
• Japanese

ID,URL

• ORCID ID

  0000-0001-5576-759X

• J-Global ID

  201401069909640685

• Researcher ID

  G-2755-2012

• External link

  https://kyouindb.iimc.kyoto-u.ac.jp/j/dP8hT

researchmap URL

• https://researchmap.jp/7000008849

Research

Research Topics, Overview of the research

• Research Topics

  Revealing plasma membrane compartmentalization and regulation of molecular function by developing ultrafast single-molecule imaging

• Overview of the research

  I have been making efforts to develop single-molecule imaging techniques in living cells, and to use the methods for understanding the functional mechanisms of the cellular plasma membrane. I am currently aiming at dual/triple-color simultaneous single fluorescent-molecule observations with a 10-nm (molecular size) spatial precision and the world’s fastest 33-microsecond temporal resolution (the ultimate frame rate for single fluorophores available today), to reveal the mechanisms for the formation and function of signaling molecular complexes at the level of single molecules in the plasma membrane compartmentalized into ~100-nm diameter domains.

Research Interests

• Function of the clellular plasma membrane
• Single-molecule nanobiology

Research Areas

• Nanotechnology/Materials, Nanobioscience
• Life sciences, Cell biology
• Life sciences, Biophysics

Papers

• Stable and Minimum Size Solubilization of Membrane Proteins with Cocktails of Phospholipid Analogues.

  Mai Takagi; Ayana Nagatani; Kenichi Kawano; Ayami Hata; Azusa Yokoyama; Koichi Hayashida; Haruka Hoshi; Mao Sakurai; Tsuyoshi Oyama; Yusuke Kuroda; Yousuke Yamaoka; Takahiro Fujiwara; Yohei Miyanoiri; Masaru Hoshino; Yoshiaki Yano; Kiyosei Takasu; Katsumi Matsuzaki

  ACS applied materials & interfaces, 07 Nov. 2024

  
• Nesprin-2 coordinates opposing microtubule motors during nuclear migration in neurons.

  Chuying Zhou; You Kure Wu; Fumiyoshi Ishidate; Takahiro K Fujiwara; Mineko Kengaku

  The Journal of cell biology, 04 Nov. 2024

  
• Transient, nano-scale, liquid-like molecular assemblies coming of age

  Akihiro Kusumi; Taka A. Tsunoyama; Kenichi G.N. Suzuki; Takahiro K. Fujiwara; Amine Aladag

  Current Opinion in Cell Biology, Aug. 2024

  
• StayGold photostability under different illumination modes.

  Masahiko Hirano; Yasuo Yonemaru; Satoshi Shimozono; Mayu Sugiyama; Ryoko Ando; Yasushi Okada; Takahiro Fujiwara; Atsushi Miyawaki

  Scientific reports, 06 Mar. 2024, Peer-reviewed

  
• Extracellular-Vesicle Catch-and-Release Isolation System Using a Net-Charge Invertible Curvature-Sensing Peptide.

  Kenichi Kawano; Yuki Kuzuma; Koichi Yoshio; Kenta Hosokawa; Yuuto Oosugi; Takahiro Fujiwara; Fumiaki Yokoyama; Katsumi Matsuzaki

  Analytical chemistry, 05 Mar. 2024, Peer-reviewed

  Access this paper :

  
• StayGold variants for molecular fusion and membrane-targeting applications

  Ryoko Ando; Satoshi Shimozono; Hideo Ago; Masatoshi Takagi; Mayu Sugiyama; Hiroshi Kurokawa; Masahiko Hirano; Yusuke Niino; Go Ueno; Fumiyoshi Ishidate; Takahiro Fujiwara; Yasushi Okada; Masaki Yamamoto; Atsushi Miyawaki

  Nature Methods, 30 Nov. 2023, Peer-reviewed

  
• Development of ultrafast camera-based single fluorescent-molecule imaging for cell biology.

  Takahiro K Fujiwara; Shinji Takeuchi; Ziya Kalay; Yosuke Nagai; Taka A Tsunoyama; Thomas Kalkbrenner; Kokoro Iwasawa; Ken P Ritchie; Kenichi G N Suzuki; Akihiro Kusumi

  The Journal of cell biology, 07 Aug. 2023, Peer-reviewed

  Access this paper :

  
• Ultrafast single-molecule imaging reveals focal adhesion nano-architecture and molecular dynamics.

  Takahiro K Fujiwara; Taka A Tsunoyama; Shinji Takeuchi; Ziya Kalay; Yosuke Nagai; Thomas Kalkbrenner; Yuri L Nemoto; Limin H Chen; Akihiro C E Shibata; Kokoro Iwasawa; Ken P Ritchie; Kenichi G N Suzuki; Akihiro Kusumi

  The Journal of cell biology, 07 Aug. 2023, Peer-reviewed

  Access this paper :

  
• Cholesterol- and actin-centered view of the plasma membrane: updating the Singer-Nicolson fluid mosaic model to commemorate its 50th anniversary†.

  Akihiro Kusumi; Taka A Tsunoyama; Bo Tang; Koichiro M Hirosawa; Nobuhiro Morone; Takahiro K Fujiwara; Kenichi G N Suzuki

  Molecular biology of the cell, 01 May 2023, Peer-reviewed

  Access this paper :

  
• A methodology for creating mutants of G‐protein coupled receptors stabilized in active state by combining statistical thermodynamics and evolutionary molecular engineering

  Taisei Yamamoto; Satoshi Yasuda; Rinshi S. Kasai; Ryosuke Nakano; Simon Hikiri; Kanna Sugaya; Tomohiko Hayashi; Satoshi Ogasawara; Mitsunori Shiroishi; Takahiro K. Fujiwara; Masahiro Kinoshita; Takeshi Murata

  Protein Science, Oct. 2022, Peer-reviewed

  
• A highly photostable and bright green fluorescent protein.

  Masahiko Hirano; Ryoko Ando; Satoshi Shimozono; Mayu Sugiyama; Noriyo Takeda; Hiroshi Kurokawa; Ryusaku Deguchi; Kazuki Endo; Kei Haga; Reiko Takai-Todaka; Shunsuke Inaura; Yuta Matsumura; Hiroshi Hama; Yasushi Okada; Takahiro Fujiwara; Takuya Morimoto; Kazuhiko Katayama; Atsushi Miyawaki

  Nature biotechnology, 25 Apr. 2022, Peer-reviewed

  
• Single-Molecule Imaging Reveals Rapid Estradiol Action on the Surface Movement of AMPA Receptors in Live Neurons

  Soma Godó; Klaudia Barabás; Ferenc Lengyel; Dávid Ernszt; Tamás Kovács; Miklós Kecskés; Csaba Varga; Tibor Z. Jánosi; Géza Makkai; Gergely Kovács; Barbara Orsolits; Takahiro Fujiwara; Akihiro Kusumi; István M. Ábrahám

  Frontiers in Cell and Developmental Biology, 23 Sep. 2021, Peer-reviewed

  
• Extracellular vesicles synchronize cellular phenotypes of differentiating cells

  Tomohiro Minakawa; Tetsuya Matoba; Fumiyoshi Ishidate; Takahiro K. Fujiwara; Sho Takehana; Yasuhiko Tabata; Jun K. Yamashita

  Journal of Extracellular Vesicles, Sep. 2021, Peer-reviewed

  Access this paper :

  
• Redox-Sensitive Cysteines Confer Proximal Control of the Molecular Crowding Barrier in the Nuclear Pore.

  Wanzhen Zhang; Ryuji Watanabe; Hide A Konishi; Takahiro Fujiwara; Shige H Yoshimura; Masahiro Kumeta

  Cell reports, 15 Dec. 2020, Peer-reviewed

  Access this paper :

  
• High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases

  Ikuko Koyama-Honda; Takahiro K. Fujiwara; Rinshi S. Kasai; Kenichi G.N. Suzuki; Eriko Kajikawa; Hisae Tsuboi; Taka A. Tsunoyama; Akihiro Kusumi

  Journal of Cell Biology, 07 Dec. 2020, Peer-reviewed

  
• Synergetic Roles of Formyl Peptide Receptor 1 Oligomerization in Ligand-Induced Signal Transduction

  Tomoki Nishiguchi; Hideaki Yoshimura; Rinshi S. Kasai; Takahiro K. Fujiwara; Takeaki Ozawa

  ACS Chemical Biology, 18 Sep. 2020, Peer-reviewed

  
• Defining raft domains in the plasma membrane.

  Kusumi A; Fujiwara TK; Tsunoyama TA; Kasai RS; Liu AA; Hirosawa KM; Kinoshita M; Matsumori N; Komura N; Ando H; Suzuki KGN

  Traffic (Copenhagen, Denmark), Jan. 2020, Peer-reviewed

  
• Dynamic actin-mediated nano-scale clustering of CD44 regulates its meso-scale organization at the plasma membrane.

  Sil P; Mateos N; Nath S; Buschow S; Manzo C; Suzuki KGN; Fujiwara T; Kusumi A; Garcia-Parajo MF; Mayor S

  Molecular biology of the cell, Oct. 2019, Peer-reviewed

  
• HsSAS-6-dependent cartwheel assembly ensures stabilization of centriole intermediates.

  Yoshiba S; Tsuchiya Y; Ohta M; Gupta A; Shiratsuchi G; Nozaki Y; Ashikawa T; Fujiwara T; Natsume T; Kanemaki MT; Kitagawa D

  Journal of cell science, Jun. 2019, Peer-reviewed

  
• Dynamic Contact Guidance of Myoblasts by Feature Size and Reversible Switching of Substrate Topography: Orchestration of Cell Shape, Orientation, and Nematic Ordering of Actin Cytoskeletons.

  Linke P; Suzuki R; Yamamoto A; Nakahata M; Kengaku M; Fujiwara T; Ohzono T; Tanaka M

  Langmuir : the ACS journal of surfaces and colloids, Nov. 2018, Peer-reviewed

  
• THE EFFECT OF LACTOFERRIN AND PEPSIN-TREATED LACTOFERRIN ON IEC-6 CELL DAMAGE INDUCED BY CLOSTRIDIUM DIFFICILE TOXIN B

  Kosuke Otake; Norio Sato; Ayako Kitaguchi; Takayuki Irahara; Satoru Murata; Keiichiro Shiraga; Yuichi Ogawa; Takahiro K. Fujiwara; Kaoru Koike; Hiroyuki Yokota

  SHOCK, Jul. 2018, Peer-reviewed

  
• Super-long single-molecule tracking reveals dynamic-anchorage-induced integrin function

  Taka A. Tsunoyama; Yusuke Watanabe; Junri Goto; Kazuma Naito; Rinshi S. Kasai; Kenichi G. N. Suzuki; Takahiro K. Fujiwara; Akihiro Kusumi

  Nature Chemical Biology, 01 May 2018, Peer-reviewed

  
• Unraveling of Lipid Raft Organization in Cell Plasma Membranes by Single-Molecule Imaging of Ganglioside Probes.

  Suzuki KGN; Ando H; Komura N; Fujiwara T; Kiso M; Kusumi A

  Advances in experimental medicine and biology, 2018, Peer-reviewed

  
• Revealing the Raft Domain Organization in the Plasma Membrane by Single-Molecule Imaging of Fluorescent Ganglioside Analogs

  Kenichi G.N. Suzuki; Hiromune Ando; Naoko Komura; Miku Konishi; Akihiro Imamura; Hideharu Ishida; Makoto Kiso; Takahiro K. Fujiwara; Akihiro Kusumi

  Methods in Enzymology, 2018, Peer-reviewed

  
• Dynamic Meso-Scale Anchorage of GPI-Anchored Receptors in the Plasma Membrane: Prion Protein vs. Thy1

  Yuri L. Nemoto; Roger J. Morris; Hiroko Hijikata; Taka A. Tsunoyama; Akihiro C. E. Shibata; Rinshi S. Kasai; Akihiro Kusumi; Takahiro K. Fujiwara

  CELL BIOCHEMISTRY AND BIOPHYSICS, Dec. 2017, Peer-reviewed

  
• The Class-A GPCR Dopamine D2 Receptor Forms Transient Dimers Stabilized by Agonists: Detection by Single-Molecule Tracking

  Rinshi S. Kasai; Shuichi V. Ito; Ryo M. Awane; Takahiro K. Fujiwara; Akihiro Kusumi

  Cell Biochemistry and Biophysics, 07 Nov. 2017, Peer-reviewed

  
• Development of new ganglioside probes and unraveling of raft domain structure by single-molecule imaging

  Kenichi G. N. Suzuki; Hiromune Ando; Naoko Komura; Takahiro K. Fujiwara; Makoto Kiso; Akihiro Kusumi

  BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, Oct. 2017, Peer-reviewed

  
• Cortical actin nodes: Their dynamics and recruitment of podosomal proteins as revealed by super-resolution and single-molecule microscopy

  Yuki M. Shirai; Taka A. Tsunoyama; Nao Hiramoto-Yamaki; Koichiro M. Hirosawa; Akihiro C. E. Shibata; Kenichi Kondo; Atsushi Tsurumune; Fumiyoshi Ishidate; Akihiro Kusumi; Takahiro K. Fujiwara

  PLOS ONE, Nov. 2017, Peer-reviewed

  
• Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs

  Masanao Kinoshita; Kenichi G. N. Suzuki; Nobuaki Matsumori; Misa Takada; Hikaru Ano; Kenichi Morigaki; Mitsuhiro Abe; Asami Makino; Toshihide Kobayashi; Koichiro M. Hirosawa; Takahiro K. Fujiwara; Akihiro Kusumi; Michio Murata

  JOURNAL OF CELL BIOLOGY, Apr. 2017, Peer-reviewed

  Access this paper :

  
• Spatiotemporal analysis with a genetically encoded fluorescent RNA probe reveals TERRA function around telomeres

  Toshimichi Yamada; Hideaki Yoshimura; Rintaro Shimada; Mitsuru Hattori; Masatoshi Eguchi; Takahiro K. Fujiwara; Akihiro Kusumi; Takeaki Ozawa

  SCIENTIFIC REPORTS, Dec. 2016, Peer-reviewed

  Access this paper :

  
• Raft-based interactions of gangliosides with a GPI-anchored receptor

  Naoko Komura; Kenichi G. N. Suzuki; Hiromune Ando; Miku Konishi; Machi Koikeda; Akihiro Imamura; Rahul Chadda; Takahiro K. Fujiwara; Hisae Tsuboi; Ren Sheng; Wonhwa Cho; Koichi Furukawa; Keiko Furukawa; Yoshio Yamauchi; Hideharu Ishida; Akihiro Kusumi; Makoto Kiso

  NATURE CHEMICAL BIOLOGY, Jun. 2016, Peer-reviewed

  
• Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane

  Takahiro K. Fujiwara; Kokoro Iwasawa; Ziya Kalay; Taka A. Tsunoyama; Yusuke Watanabe; Yasuhiro M. Umemura; Hideji Murakoshi; Kenichi G. N. Suzuki; Yuri L. Nemoto; Nobuhiro Morone; Akihiro Kusumi

  MOLECULAR BIOLOGY OF THE CELL, Apr. 2016, Peer-reviewed

  Access this paper :

  
• Selective Labeling of Proteins on Living Cell Membranes Using Fluorescent Nanodiamond Probes

  Shingo Sotoma; Jun Iimura; Ryuji Igarashi; Koichiro M. Hirosawa; Hidenori Ohnishi; Shin Mizukami; Kazuya Kikuchi; Takahiro K. Fujiwara; Masahiro Shirakawa; Hidehito Tochio

  NANOMATERIALS, Apr. 2016, Peer-reviewed

  Access this paper :

  
• Leader-Containing Uncapped Viral Transcript Activates RIG-I in Antiviral Stress Granules

  Seong-Wook Oh; Koji Onomoto; Mai Wakimoto; Kazuhide Onoguchi; Fumiyoshi Ishidate; Takahiro Fujiwara; Mitsutoshi Yoneyama; Hiroki Kato; Takashi Fujita

  PLOS PATHOGENS, Feb. 2016, Peer-reviewed

  Access this paper :

  
• Tracking single molecules at work in living cells

  Akihiro Kusumi; Taka A. Tsunoyama; Kohichiro M. Hirosawa; Rinshi S. Kasai; Takahiro K. Fujiwara

  NATURE CHEMICAL BIOLOGY, Jul. 2014, Peer-reviewed

  
• Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes

  Nao Hiramoto-Yamaki; Kenji A. K. Tanaka; Kenichi G. N. Suzuki; Koichiro M. Hirosawa; Manami S. H. Miyahara; Ziya Kalay; Koichiro Tanaka; Rinshi S. Kasai; Akihiro Kusumi; Takahiro K. Fujiwara

  TRAFFIC, Jun. 2014, Peer-reviewed

  
• Lateral diffusion in a discrete fluid membrane with immobile particles

  Ziya Kalay; Takahiro K. Fujiwara; Akihisa Otaka; Akihiro Kusumi

  Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, 26 Feb. 2014, Peer-reviewed

  
• Transient GPI-anchored protein homodimers are units for raft organization and function

  Kenichi G. N. Suzuki; Rinshi S. Kasai; Koichiro M. Hirosawa; Yuri L. Nemoto; Munenori Ishibashi; Yoshihiro Miwa; Takahiro K. Fujiwara; Akihiro Kusumi

  NATURE CHEMICAL BIOLOGY, Sep. 2012, Peer-reviewed

  
• Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone

  Akihiro C. E. Shibata; Takahiro K. Fujiwara; Limin Chen; Kenichi G. N. Suzuki; Yoshiro Ishikawa; Yuri L. Nemoto; Yoshihiro Miwa; Ziya Kalay; Rahul Chadda; Keiji Naruse; Akihiro Kusumi

  CYTOSKELETON, Jun. 2012, Peer-reviewed

  
• Confining Domains Lead to Reaction Bursts: Reaction Kinetics in the Plasma Membrane

  Ziya Kalay; Takahiro K. Fujiwara; Akihiro Kusumi

  PLOS ONE, Mar. 2012, Peer-reviewed

  Access this paper :

  
• Dynamic Organizing Principles of the Plasma Membrane that Regulate Signal Transduction: Commemorating the Fortieth Anniversary of Singer and Nicolson's Fluid-Mosaic Model

  Akihiro Kusumi; Takahiro K. Fujiwara; Rahul Chadda; Min Xie; Taka A. Tsunoyama; Ziya Kalay; Rinshi S. Kasai; Kenichi G. N. Suzuki

  ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 28, 2012, Peer-reviewed

  
• Hierarchical mesoscale domain organization of the plasma membrane

  Akihiro Kusumi; Kenichi G. N. Suzuki; Rinshi S. Kasai; Ken Ritchie; Takahiro K. Fujiwara

  TRENDS IN BIOCHEMICAL SCIENCES, Nov. 2011, Peer-reviewed

  
• Full characterization of GPCR monomer-dimer dynamic equilibrium by single molecule imaging

  Rinshi S. Kasai; Kenichi G. N. Suzuki; Eric R. Prossnitz; Ikuko Koyama-Honda; Chieko Nakada; Takahiro K. Fujiwara; Akihiro Kusumi

  JOURNAL OF CELL BIOLOGY, Feb. 2011, Peer-reviewed

  
• Membrane molecules mobile even after chemical fixation

  Kenji A. K. Tanaka; Kenichi G. N. Suzuki; Yuki M. Shirai; Shusaku T. Shibutani; Manami S. H. Miyahara; Hisae Tsuboi; Miyako Yahara; Akihiko Yoshimura; Satyajit Mayor; Takahiro K. Fujiwara; Akihiro Kusumi

  NATURE METHODS, Nov. 2010, Peer-reviewed

  Access this paper :

  
• GPI-anchored receptor clusters transiently recruit Lyn and G alpha for temporary cluster immobilization and Lyn activation: single-molecule tracking study 1

  Kenichi G. N. Suzuki; Takahiro K. Fujiwara; Fumiyuki Sanematsu; Ryota Iino; Michael Edidin; Akihiro Kusumi

  JOURNAL OF CELL BIOLOGY, May 2007, Peer-reviewed

  
• Dynamic recruitment of phospholipase C gamma at transiently immobilized GPI- anchored receptor clusters induces IP3-Ca2+ signaling: single-molecule tracking study 2

  Kenichi G. N. Suzuki; Takahiro K. Fujiwara; Michael Edidin; Akihiro Kusumi

  JOURNAL OF CELL BIOLOGY, May 2007, Peer-reviewed

  
• Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography

  Nobuhiro Morone; Takahiro Fujiwara; Kotono Murase; Rinshi S. Kasai; Hiroshi Ike; Shigeki Yuasa; Jiro Usukura; Akihiro Kusumi

  JOURNAL OF CELL BIOLOGY, Sep. 2006, Peer-reviewed

  Access this paper :

  
• Detection of non-Brownian diffusion in the cell membrane in single molecule tracking

  K Ritchie; XY Shan; J Kondo; K Iwasawa; T Fujiwara; A Kusumi

  BIOPHYSICAL JOURNAL, Mar. 2005, Peer-reviewed

  
• Fluorescence imaging for monitoring the colocalization of two single molecules in living cells

  Koyama-Honda, I; K Ritchie; T Fujiwara; R Iino; H Murakoshi; RS Kasai; A Kusumi

  BIOPHYSICAL JOURNAL, Mar. 2005, Peer-reviewed

  
• Single-molecule imaging of diffusion, recruitment, and activation of signaling molecules in living cells.

  A. Kusumi; H. Murakoshi; K. Murase; T. Fujiwara

  In “Biophysical aspects of transmembrane signaling”, Chapter 5, S. Damjanovich, Ed. Springer-Heidelberg pp. 123-152.（2005）., 2005, Peer-reviewed, Invited

  
• Paradigm shift of the plasma membrane concept from the two-dimensional continuum fluid to the partitioned fluid: High-speed single-molecule tracking of membrane molecules

  A Kusumi; C Nakada; K Ritchie; K Murase; K Suzuki; H Murakoshi; RS Kasai; J Kondo; T Fujiwara

  ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 2005, Peer-reviewed

  
• Ultrafine membrane compartments for molecular diffusion as revealed by single molecule techniques

  K Murase; T Fujiwara; Y Umemura; K Suzuki; R Iino; H Yamashita; M Saito; H Murakoshi; K Ritchie; A Kusumi

  BIOPHYSICAL JOURNAL, Jun. 2004, Peer-reviewed

  
• Single-molecule imaging analysis of Ras activation in living cells

  H Murakoshi; R Iino; T Kobayashi; T Fujiwara; C Ohshima; A Yoshimura; A Kusumi

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, May 2004, Peer-reviewed

  
• Accumulation of anchored proteins forms membrane diffusion barriers during neuronal polarization

  C Nakada; K Ritchie; Y Oba; M Nakamura; Y Hotta; R Iino; RS Kasai; K Yamaguchi; T Fujiwara; A Kusumi

  NATURE CELL BIOLOGY, Jul. 2003, Peer-reviewed

  
• Phospholipids undergo hop diffusion in compartmentalized cell membrane

  T Fujiwara; K Ritchie; H Murakoshi; K Jacobson; A Kusumi

  JOURNAL OF CELL BIOLOGY, Jun. 2002, Peer-reviewed

  
• Oscillatory Control of Factors Determining Multipotency and Fate in Mouse Neural Progenitors

  Itaru Imayoshi; Akihiro Isomura; Yukiko Harima; Kyogo Kawaguchi; Hiroshi Kori; Hitoshi Miyachi; Takahiro Fujiwara; Fumiyoshi Ishidate; Ryoichiro Kageyama

  SCIENCE, Dec. 2013, Peer-reviewed

  Access this paper :

  
• Biexciton state causes photoluminescence fluctuations in CdSe/ZnS core/shell quantum dots at high photoexcitation densities

  N. Yoshikawa; H. Hirori; H. Watanabe; T. Aoki; T. Ihara; R. Kusuda; C. Wolpert; T. K. Fujiwara; A. Kusumi; Y. Kanemitsu; K. Tanaka

  Physical Review B - Condensed Matter and Materials Physics, 31 Oct. 2013, Peer-reviewed

  
• Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site

  Ikuko Koyama-Honda; Eisuke Itakura; Takahiro K. Fujiwara; Noboru Mizushima

  AUTOPHAGY, Oct. 2013, Peer-reviewed

  
• Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging

  Hirohito Nishimura; Ken Ritchie; Rinshi S. Kasai; Nobuhiro Morone; Hiroyuki Sugimura; Koichiro Tanaka; Ichiro Sase; Akihiko Yoshimura; Yoshitaro Nakano; Takahiro K. Fujiwara; Akihiro Kusumi

  JOURNAL OF CELL BIOLOGY, Sep. 2013, Peer-reviewed

  
• Rac1 recruitment to the archipelago structure of the focal adhesion through the fluid membrane as revealed by single-molecule analysis

  Akihiro C.E. Shibata; Limin H. Chen; Rie Nagai; Fumiyoshi Ishidate; Rahul Chadda; Yoshihiro Miwa; Keiji Naruse; Yuki M. Shirai; Takahiro K. Fujiwara; Akihiro Kusumi

  Cytoskeleton, Mar. 2013, Peer-reviewed

  
• Functional Characterization of Domains of IPS-1 Using an Inducible Oligomerization System

  Shiori Takamatsu; Kazuhide Onoguchi; Koji Onomoto; Ryo Narita; Kiyohiro Takahasi; Fumiyoshi Ishidate; Takahiro K. Fujiwara; Mitsutoshi Yoneyama; Hiroki Kato; Takashi Fujita

  PLoS ONE, 15 Jan. 2013, Peer-reviewed

  Access this paper :

  
• Single-Molecule Imaging of Receptor-Receptor Interactions

  Kenichi G. N. Suzuki; Rinshi S. Kasai; Takahiro K. Fujiwara; Akihiro Kusumi

  RECEPTOR-RECEPTOR INTERACTIONS, 2013, Peer-reviewed, Invited

  
• Cholesterol modulates cell signaling and protein networking by specifically interacting with PDZ domain-containing scaffold proteins

  Ren Sheng; Yong Chen; Heon Yung Gee; Ewa Stec; Heather R. Melowic; Nichole R. Blatner; Moe P. Tun; Yonjung Kim; Morten Kaellberg; Takahiro K. Fujiwara; Ji Hye Hong; Kwang Pyo Kim; Hui Lu; Akihiro Kusumi; Min Goo Lee; Wonhwa Cho

  NATURE COMMUNICATIONS, Dec. 2012, Peer-reviewed

  
• Membrane mechanisms for signal transduction: The coupling of the meso-scale raft domains to membrane-skeleton-induced compartments and dynamic protein complexes

  Akihiro Kusumi; Takahiro K. Fujiwara; Nobuhiro Morone; Kenta J. Yoshida; Rahul Chadda; Min Xie; Rinshi S. Kasai; Kenichi G. N. Suzuki

  SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, Apr. 2012, Peer-reviewed

  
• Cytoskeleton-Induced Mesoscale Domains

  Ziya Kalay; Takahiro K. Fujiwara; Akihiro Kusumi

  Cellular Domains, 31 May 2011, Peer-reviewed, Invited

  
• Hierarchical organization of the plasma membrane: Investigations by single-molecule tracking vs. fluorescence correlation spectroscopy

  Akihiro Kusumi; Yuki M. Shirai; Ikuko Koyama-Honda; Kenichi G. N. Suzuki; Takahiro K. Fujiwara

  FEBS LETTERS, May 2010, Peer-reviewed

  Access this paper :

  
• Paradigm Shift of the Molecular Dynamics Concept in the Cell Membrane: High-Speed Single-Molecule Tracking Revealed the Partitioning of the Cell Membrane

  Akihiro Kusumi; Yasuhiro Umemura; Nobuhiro Morone; Takahiro Fujiwara

  Anomalous Transport: Foundations and Applications, 30 Sep. 2008, Peer-reviewed, Invited

  
• Both MHC class II and its GPI-anchored form undergo hop diffusion as observed by single-molecule tracking

  Yasuhiro M. Umemura; Marija Vrljic; Stefanie Y. Nishimura; Takahiro K. Fujiwara; Kenichi G. N. Suzuki; Akihiro Kusumi

  BIOPHYSICAL JOURNAL, Jul. 2008, Peer-reviewed

  Access this paper :

  
• Rapid hop diffusion of a G-protein-coupled receptor in the plasma membrane as revealed by single-molecule techniques

  K Suzuki; K Ritchie; E Kajikawa; T Fujiwara; A Kusumi

  BIOPHYSICAL JOURNAL, May 2005, Peer-reviewed

  
• Single-molecule tracking of membrane molecules: plasma membrane compartmentalization and dynamic assembly of raft-philic signaling molecules

  A Kusumi; H Ike; C Nakada; K Murase; T Fujiwara

  SEMINARS IN IMMUNOLOGY, Feb. 2005, Peer-reviewed

  
• Mechanism of Lck recruitment to the T-cell receptor cluster as studied by single-molecule-fluorescence video Imaging

  H Ike; A Kosugi; A Kato; R Iino; H Hirano; T Fujiwara; K Ritchie; A Kusumi

  CHEMPHYSCHEM, Jun. 2003, Peer-reviewed

  
• The fence and picket structure of the plasma membrane of live cells as revealed by single molecule techniques (Review)

  K Ritchie; R Iino; T Fujiwara; K Murase; A Kusumi

  MOLECULAR MEMBRANE BIOLOGY, Jan. 2003, Peer-reviewed

  
• Relationship of lipid rafts to transient confinement zones detected by single particle tracking

  C Dietrich; B Yang; T Fujiwara; A Kusumi; K Jacobson

  BIOPHYSICAL JOURNAL, Jan. 2002, Peer-reviewed

  
• LS3A1 Diffusion barrier in the neuronal cell membrane : a single molecule study

  Nakada C.; Ritchie Kenneth; Fujiwara T.; Nakamura M.; Oba Y.; Hotta Y.; Iino R.; Yamaguchi K.; Kusumi A.

  Seibutsu Butsuri, 2002

  
• 3N1415 Single molecule imaging of trimeric G-Protein activation by a formyl peptide receptor

  Kasai R.; Iino R.; Fujiwara T.; Kobayashi T.; Prossnitz Eric; Kusumi A.

  Seibutsu Butsuri, 2002

  
• 3N1515 Stabilization of rafts in signal transduction via CD-14 as studied by single molecule observations

  Mizoguchi H.; Hotta Y.; Suzuki K.; Kenneth Ritchic; Sanematsu F.; Fujiwara T.; Kusumi A.

  Seibutsu Butsuri, 2002

  
• 3N1500 Inter-leaflet conpling of lipid raft molecules in the cell membrane

  Koyama I.; Iino R.; Murakoshi H.; Fujiwara T.; Kajikawa E.; Yoshimura A.; Kobayashi T.; Kusumi A.

  Seibutsu Butsuri, 2002

  
• S3B5 Stimulation-induced formation of transient but stabilized rafts.(Single Molecure Dynamics and Reactions)

  Suzuki Kenichi; Sanematsu Fumiyuki; Fujiwara Takahiro; Edidin Michael; Kusumi Akihiro

  Seibutsu Butsuri, 2002

  
• 2N1600 A new internalization pathway delivers activated SCF receptor into signaling endocytic vesicles : discovery by a single molecule method

  Kobayashi T.; Murakami M.; Takeda Y.; Yasui-Ohshima C.; Irie K.; Sakuma M.; Fujiwara T.; Kawasaki T.; Yoshimura A.; Kusumi A.

  Seibutsu Butsuri, 2002

  
• 2N1545 Cooperative assembly of clathrin-coated pits as revealed by single fluorophore video observations

  Fujiwara T.; Iino R.; Ohshima C.; Kobayashi T.; Keen James; Kusumi A.

  Seibutsu Butsuri, 2002

  
• 2L1600 Single Molecule Detection of H-Ras Activation and Localization by Single Molecule Fluorescence Resonance Energy Transfer Method

  Murakoshi H.; Iino R.; Kobayashi T.; Fujiwara T.; Ohshima C.; Murakami M.; Yoshimura A.; Kusumi A.

  Seibutsu Butsuri, 2002

  
• 2L1615 Activation of GP1-anchored protein CD59 induces temporarily stabilized signaling rafts from small transient rafts.

  Suzuki K.; Sanematsu F.; Fujiawra T.; Edidin Michael; Kusumi A.

  Seibutsu Butsuri, 2002

  
• 2L1545 Regulation mechanism for the movement of a transmembrane protein BP180 as studied by single molecule techniques

  Murase K.; Hirako Y.; Fujiwara T.; Iino R.; Owaribe K.; Ritchie K.; Kusumi A.

  Seibutsu Butsuri, 2002

  
• 1H0920 Single molecule video observations of GPCR signaling

  Kasai R.; Iino R.; Fujiwara T.; Kobayashi T.; Prossnitz Eric

  Seibutsu Butsuri, 2002

  
• 1H0900 Regulation mechanism for the movement of transmembrane proteins and phospholipids : An approach by single molecule techniques

  Murase K.; Hirako Y.; Fujiwara T.; Iino R.; Owaribe K.; Ritchie K.; Kusumi A.

  Seibutsu Butsuri, 2002

  
• 1H0940 Single molecule observation of three types of GPI-anchored-protein associated rafts.

  Suzuki K.; Sanematsu F.; Fujiwara T.; Edidin Michael; Kusumi A.

  Seibutsu Butsuri, 2002

  
• 1H1020 SIngle molecule imaging of recruitment and localization of adaptor and receptor molecules into clathrin-coated pits

  Fujiwara T.; Iino R.; Ohshima C.; Kobayashi T.; Keen James; Kusumi A.

  Seibutsu Butsuri, 2002

  
• 2N1400 Accumulation of anchored proteins forms membrane diffusion barriers as neurons develop polariza-

  Nakada C.; Ritchie Kenneth; Fujiwara T.; Nakamura M.; Ooba Y.; Hotta Y.; Iino R.; Yamaguchi K.; Kusumi A.

  Seibutsu Butsuri, 2002

  
• Formation mechanism of a diffusion barrier in the initial segment membrane of the neuron as studied by single molecule techniques

  Nakada C.; Ritchie Kenneth; Fujiwara T.; Hotta Y.; Iino R.; Yamaguchi K.; Kusumi A.

  Seibutsu Butsuri, 2001

  
• Raft-like domains are unstable(a few molecules/milliseconds)on resting cell membranes

  Suzuki K.; Sanematsu F.; Fujiwara T.; Edidin M.; Kusumi A.

  Seibutsu Butsuri, 2001

  
• Developmental formation of a diffusion barrier in the axonal initial segment membrane of the neuron ; a single molecule approach.

  Nakada C.; Ritchie Kenneth; Fujiwara T.; Hotta Y.; Iino R.; Yamagucho K.; Kusumi A.

  Seibutsu Butsuri, 2001

  
• Generation of diffusion barriers in the plasma membrane plane : A Monte Carlo study

  Kenneth Ritchie; Fujiwara T; Nakada C; Murase K; Kusumi A

  Seibutsu Butsuri, 2001

  
• Activated GPI-anchored membrane protein, CD59, frequently visits/forms rafts which depend on SFK activity and actin

  Hirano H.; Suzuki K.; Sanematsu F.; Fujiwara T.; Tsuboi H.; Aihara M.; Kusumi A.

  Seibutsu Butsuri, 2001

  
• Single molecule imaging of localization processes of receptors and adaptor molecules AP2 into clathrin-coated pits

  Fujiwara T.; Murakoshi H.; Iino R.; Gaidarov Ibragim; Keen James; Kusumi A.

  Seibutsu Butsuri, 2001

  
• Localization mechanism for G protein-chemotactic receptor signaling : single molecule imaging of G-protein activation

  Kasai R.; Murakoshi H.; Iino R.; Fujiwara T.; Nakanishi H.; Yoshizumi R.; Itami N.; Kobayashi T.; Haga T.; Prossnitz Eric; Kusumi A.

  Seibutsu Butsuri, 2001

  
• Regulation mechanism for the assembly of adaptor molecules and receptors in clathrin-coated pits as studied by single fluorophore imaging

  Fujiwara T.; Murakosi H.; Iino R.; Gaidarov Ibragim; Keen James; Kusumi A.

  Seibutsu Butsuri, 2001

  
• Hop diffusion of phospholipid in the cell membrane as studied by single molecule techniques : Hops among 40 nm compartments every 5 ms

  Murase K; Fujiwara T; Iino R; Murakoshi H; Rithcie Ken; Kusumi A

  Seibutsu Butsuri, 2001

  
• Single molecule analysis of G-protein-coupled chemotaxis

  Kasai R.; Murakoshi H.; Iino R.; Fujiwara T.; Nakanishi H.; Yoshizumi R.; Itami N.; Kobayashi T.; Haga T.; Prossnitz Eric; Kusumi A.

  Seibutsu Butsuri, 2001

  
• Spatial regulation of stem cell factor(SCF)-SCF receptor signaling in melanocytes

  Kobayashi T.; Murakami M.; Irie K.; Sakuma M.; Ohsima C.; Takeda Y.; Iino R.; Fujiwara T.; Yoshimura A.; Kusumi A.

  Seibutsu Butsuri, 2001

  
• Fences and pickets formed by the membrane skeleton and its anchored membrane proteins are universally found in various cultured cells

  Kondo J; Fujiwara T; Iino R; Murase K; Yamashita H; Saito M; Ike H; Murakoshi H; Suzuki K; Kusumi A

  Seibutsu Butsuri, 2001

  
• Imaging analysis of frequent visits of a GPI-anchored protein CD59 on rafts in signal transduction

  Suzuki K.; Sanematsu F.; Fujiwara T.; Kusumi A.

  Seibutsu Butsuri, 2000

  
• Compartmentalization of the plasma membrane by the pickets of membrane skeletonanchored proteins slows the diffusion of phospholipids.

  Fujiwara T.; Murase K.; Ritchie Ken; Kusumi A

  Seibutsu Butsuri, 2000

  
• Double-compartmentalization of the movement of phospholipids in the plasma membrane through anchored-protein pickets

  Fujiwara T.; Ritchie Ken; Kusumi A.

  Seibutsu Butsuri, 2000

  
• 50nm-compartments in the plasma membrane by anchored-protein pickets, which cause slowing of the diffusion of phospholipids

  Murase K.; Fujiwara T.; Kusumi A.

  Seibutsu Butsuri, 2000

  
• Frequent visits of a GPI-Ancholred portein CD59 on rafts during signaling events

  Suzuki K.; Sanematsu F.; Fujiwara T.; Kusumi A.

  Seibutsu Butsuri, 2000

  
• Diffusinal movement of SCF receptor in melanocyte membranes as studied by single molecule tracking.

  Kobayashi T.; Murakami M.; Irie K.; Iino R.; Fujiwara T.; Kusumi A.

  Seibutsu Butsuri, 2000

  
• Compartmentalized movement of phospholipids in the plasma membrane : anchored-protein picket model.

  Fujiwara T.; Kusumi A.

  Seibutsu Butsuri, 1999

  
• Application of laser tweezers to studies of the fences and tethers of the membrane skeleton that regulate the movements of plasma membrane proteins

  A Kusumi; Y Sako; T Fujiwara; M Tomishige

  METHODS IN CELL BIOLOGY, VOL 55, 1998, Peer-reviewed

  

Misc.

• Development of Ultrafast Camera-based Single-Molecule Imaging Unravels Plasma Membrane Structure and Function in Live Cells

  Takahiro Fujiwara; Akihiro Kusumi

  生物物理, Oct. 2024, Peer-reviewed, Invited

  
• 新しい細胞膜構造

  諸根 信弘; 藤原 敬宏; 笠井 倫志; 臼倉 治郎; 湯浅 茂樹; 楠見 明弘

  バイオイメージング, 31 Oct. 2006

  
• 1分子検出蛍光顕微鏡

  藤原敬宏

  先端の分析法 第2版 エヌ・ティー・エス, Jan. 2022, Invited

  
• 暗視野観察

  藤原 敬宏

  実験医学増刊 生きてるものは全部観る! イメージングの選び方・使い方100+, Dec. 2018, Invited

  
• 光ピンセット

  楠見明弘; 藤原 敬宏

  ライフサイエンス 顕微鏡学ハンドブック 朝倉書店, Jan. 2018, Invited

  
• 超高速・超解像1蛍光分子局在顕微鏡法

  楠見明弘; 藤原敬宏

  日本免疫学会ニュースレター, 2014, Invited

  
• １分子イメージングによる細胞膜シグナル変換機構の解明

  楠見明弘; 鈴木健一; 藤原敬宏; 笠井倫志

  生体の科学, 2013, Invited

  
• 超高速･超解像１蛍光分子顕微鏡

  藤原敬宏; 笠井倫志; 楠見明弘

  現代化学, 2013, Invited

  
• シグナル伝達の1 分子イメジング

  楠見明弘; 笠井倫志; 吉田謙太; 廣澤幸一朗; 藤原敬宏; 鈴木健一

  実験医学 増刊「シグナル伝達研究最前線2012」, 2012, Invited

  
• トランスポートソームの組織化における細胞骨格系の役割

  木下専; 藤原敬宏

  トランスポートソームの世界－膜輸送研究の源流から未来へ－, 2011, Invited

  
• ランダムな運動に法則を見出す

  楠見明弘; 藤原敬宏

  math stories 変化をとらえる, 2009, Invited

  
• 高速１粒子追跡法

  藤原敬宏

  非侵襲・可視化技術ハンドブック－ナノ・バイオ・医療から情報システムまで－, 2007, Invited

  
• The Slowing Mechanism of Phospholipid Diffusion in the Cell Membrane : Anchored Membrane-protein Picket Model

  FUJIWARA Takahiro; KUSUMI Akihiro

  MEMBRANE, 2005, Invited

  
• アダプター分子の集合からみたクラスリン被覆ピットの形成過程 ～一分子法によるアプローチ～

  藤原敬宏

  実験医学別冊 バイオイメージングでここまで理解(わか)る, 2003, Invited

  
• １分子のリン脂質の運動をも抑制する細胞膜中の仕切り

  藤原敬宏

  実験医学別冊 バイオイメージングでここまで理解(わか)る, 2003, Invited

  
• 1分子蛍光共鳴エネルギー移動法による生細胞内でのH-Ras活性化のイメージング

  村越 秀治; 飯野 亮太; 小林 剛; 藤原 敬宏; 大島 智香; 村上 瑞奈; 吉村 昭彦; 楠見 明弘

  バイオイメージング, 21 Oct. 2002

  
• ラフトによる細胞膜外層-内層シグナル分子のカップリングの可視化解析

  小山 郁子; 飯野 亮太; 村越 秀治; 藤原 敬宏; 梶川 絵理子; 吉村 昭彦; 小林 剛; 楠見 明弘

  バイオイメージング, 21 Oct. 2002

  
• ヘミデスモソーム膜タンパク質BP180の拡散抑制機構の1分子法による研究

  村瀬 琴乃; 平子 善章; 藤原 敬宏; 飯野 亮太; 尾張部 克志; リッチー ケン; 楠見 明弘

  バイオイメージング, 21 Oct. 2002

  
• 光ピンセットによる膜タンパク質の1分子操作

  藤原敬宏; Ken Ritchie; 山下英俊; 楠見明弘

  ナノ光工学ハンドブック, 2002, Invited

  
• Regulation Mechanisms for Molecular Diffusion in the Cell Membrane by the Membrane Skeleton : A Single Molecule Approach

  MURASE Kotono; RITCHIE Kenneth; FUJIWARA Takahiro; IINO Ryota; NAKADA Chieko; KUSUMI Akihiro

  MEMBRANE, 2002, Invited

  
• 細胞膜上での1分子観察と操作―細胞膜上のコンパートメントやドメインの構造と機能を調べる

  藤原敬宏; Ken Ritchie; 鈴木健一

  細胞工学, 2001, Invited

  
• 生細胞中での光ピンセットによる１分子操作

  藤原敬宏; 楠見明弘

  バイオイメージング技術の最先端, 1999, Invited

  
• 光ピンセットによる１分子操作 [光ピンセットの原理と分子操作の実例]

  藤原敬宏; 楠見明弘

  シリーズ・光が拓く生命科学, 1999, Invited

  
• 生細胞中での１分子計測と制御

  楠見明弘; 藤原敬宏

  光学, 1999, Invited

  
• Regulation mechanisms of the movements of membrane proteins and lipids as studied by laser tweezers and single particle tracking.

  Fujiwara T.; Tomishige M.; Nakada C.; Kusumi A.

  Biophysics, 07 Sep. 1998

  
• Intercompartmental hop diffusion of a phospholipid in the plasma membrane as studied by single particle tracking.

  Fujiwara T.; Kusumi A.

  Biophysics, 07 Sep. 1998

  
• 光ピンセット法とその細胞膜研究への応用

  佐甲靖志; 富重道雄; 藤原敬宏

  ファルマシア, 1996, Invited

  
• デスモソームの生物学

  楠見明弘; 藤原敬宏

  医学のあゆみ, 1993, Invited

  
• 細胞膜タンパク質の運動、会合、集合と 機能

  楠見明弘; 佐甲靖志; 藤原敬宏

  Annual Review 細胞生物学, 1993, Invited

  

Presentations

• 生体膜構造の超解像観察が拓く新しい世界

  藤原敬宏

  日本化学会 第14回 CSJ化学フェスタ2024 テーマ企画「細胞外小胞の新しい世界～細胞からのメッセージとその活用～」, 23 Oct. 2024, Invited

  
• 超高速1蛍光分子観察による接着斑メゾ構造分子動態の解明

  藤原敬宏

  定量生物学の会 第11回年会 セッション1 「時間と空間の限界を突破する」, 06 Jan. 2024, Invited

  
• Mesoscopic architecture and molecular dynamics in focal adhesions as revealed by ultrafast single-molecule imaging

  Takahiro Fujiwara

  第61回日本生物物理学会年会 シンポジウム3SEA「細胞のメゾ構造体の形成と機能の機構：先端イメジング法による解明」, 16 Nov. 2023, Invited

  
• Mesoscopic architecture and molecular dynamics in focal adhesions as revealed by ultrafast single-molecule imaging

  T. Fujiwara

  The East Asia Single Molecule Biology Symposium 2023 (EASMB 2023), 22 Sep. 2023, Invited

  
• Unraveling mesoscopic organization of the plasma membrane by ultrafast single fluorescent-molecule tracking and localization microscopy

  Takahiro Fujiwara

  NanoLSI Open Seminar at NanoLSI, Kanazawa University, 12 Nov. 2021, Invited

  
• Organization of focal adhesion based on actin-induced compartments and dynamic protein islands as revealed by ultrafast single-molecule imaging

  T. Fujiwara

  1st WPI NanoLSI-iCeMS Joint Symposium on Nanoimaging and Advanced Materials for Life Science, 23 Jan. 2020, Invited

  
• Actin-induced compartments and islands in focal adhesions as revealed by simultaneous ultrafast PALM and single-molecule tracking

  Takahiro Fujiwara

  第57回日本生物物理学会年会 シンポジウム3SGA「超解像顕微鏡による生物物理学的生理学・病理学」, 26 Sep. 2019, Invited

  
• Dynamic entrance and exit of integrin molecules at focal adhesion protein islands as revealed by super-spatiotamporal resolution single-molecule microscopy

  Takahiro Fujiwara

  Academia Sinica-Kyoto University Bilateral Symposium: Cellular and Molecular Sensing, Recognition and Response, 24 Jun. 2019, Invited

  
• Actin-induced compartmentalization of the channels between the focal-adhesion-protein islands as revealed by simultaneous ultrafast PALM and single-molecule tracking

  T. Fujiwara

  16th International Membrane Research Forum, 18 Mar. 2019, Invited

  
• Ultrafast single fluorescent-molecule imaging reveals hop diffusion within focal adhesion: actin-induced compartmentalization of the channels between the focal-adhesion-protein islands

  T. Fujiwara; A. Kusumi

  Cold Spring Harbor Laboratory meeting on Single Biomolecules, 31 Aug. 2018

  
• Revealing mesoscopic organization and function of the plasma membrane by developing high-speed single-molecule microscopy

  T. Fujiwara

  Bangalore Microscopy Course 2017, Bangalore, India, 23 Sep. 2017, Invited

  
• Revealing plasma membrane compartmentalization and function: an ultrafast single-molecule imaging study

  T. Fujiwara

  The UK-Japan Spring Neuroscience Symposium, Kyoto, 16 Mar. 2017, Invited

  
• Revealing plasma membrane organization by developing high-speed single-molecule imaging methods

  藤原 敬宏

  CREST 永井チーム・ワークショップ 『超解像「生理機能」イメージング法の開発と細胞状態解析への応用』、沖縄, 27 Jul. 2016, Invited

  
• Plasma membrane compartmentalization and function as revealed by high-speed single-molecule microscopy

  T. Fujiwara

  Single Molecule Workshop: Theory Meets Experiment, Telluride, USA, 15 Jul. 2016, Invited

  
• Mesoscopic organization and function of the plasma membrane: an ultrafast single-molecule imaging study

  T. Fujiwara

  The 2016 Gordon Research Conference on Single Molecule Approaches to Biology, Single-Molecule Microscopy: Life at a Higher Resolution, Hong Kong, China, 07 Jul. 2016, Invited

  
• Revealing mesoscopic organization of the plasma membrane by developing high-speed single-molecule localization and tracking techniques

  T. Fujiwara

  Spectroscopical Society of Japan International Symposium: Advanced spectroscopy and imaging with nano-scale resolution, Osaka, 25 May 2016, Invited

  
• Plasma membrane compartmentalization and function as revealed by ultrafast single fluorescent-molecule imaging

  T. Fujiwara

  Cold Spring Harbor Asia Conferences on New Advances in Optical Imaging of Live Cells and Organisms, Suzhou, China, 09 Dec. 2015, Invited

  
• 1分子局在超解像法による細胞内動態の理解に向けて

  藤原敬宏

  日本顕微鏡学会第71回学術講演会 チュートリアルセッションT4「超解像蛍光顕微鏡法のための試料調製と像解釈」、京都, 15 May 2015, Invited

  
• Revealing plasma membrane compartmentalization and function by developing ultrafast single-molecule imaging techniques

  T. Fujiwara

  The 9th Asian Biophysics Association Symposium (ABA2015), Hangzhou, China, 10 May 2015, Invited

  
• Mesoscopic organization of the plasma membrane as revealed by the development of ultrafast single-fluorescent molecule imaging

  T. Fujiwara

  15th International Membrane Research Forum, Kyoto, 02 Mar. 2015, Invited

  
• Dynamic regulation of molecular diffusion in compartmentalized plasma membrane as revealed by ultrafast single-molecule imaging

  T. Fujiwara

  Institute for Protein Research Seminar "Molecular Crowding and Macromolecular Association", OSAKA, 05 Feb. 2015, Invited

  
• Revealing plasma membrane organization and function by developing ultrafast single-molecule tracking and localization techniques

  T. Fujiwara

  Biophysics Colloquia at Cornell University, Ithaca, USA, 21 Jan. 2015, Invited

  
• Mesoscopic organization and function of the plasma membrane as revealed by ultrafast single-molecule localization microscopy

  T. Fujiwara

  Chemistry and Randall Seminar at King’s College London, London, UK, 16 Jan. 2015, Invited

  
• Plasma membrane compartmentalization and function: an ultrafast single-molecule imaging study

  T. Fujiwara

  Structural Biology Seminar Series at MRC National Institute for Medical Research, London, UK, 15 Jan. 2015, Invited

  
• Mesoscopic organization and function of the plasma membrane as revealed by ultrafast single-molecule localization microscopy

  T. Fujiwara

  細胞のメゾスケール構造機能シンポジウム、京都, 13 Dec. 2014, Invited

  
• Mesoscopic organization and function of the plasma membrane as revealed by ultrafast single-fluorescent molecule localization microscopy

  T. Fujiwara

  2013 University System of Taiwan-Kyoto University iCeMS International Symposium, Hsinchu, Taiwan, 18 Nov. 2013, Invited

  
• Hop diffusion in the plasma membrane as detected by ultrafast single-molecule localization

  T. Fujiwara

  14th International Membrane Research Forum, Kyoto, 16 Mar. 2013

  
• Hop diffusion in the plasma membrane as detected by ultrafast single-molecule localization

  T. Fujiwara

  14th International Membrane Research Forum, Kyoto, 06 Mar. 2013, Invited

  
• Hop diffusion in the plasma membrane as revealed by ultrafast camera for single fluorescent-molecule localization

  T. Fujiwara

  日本バイオイメージング学会 第21回学術集会 シンポジウムII, 27 Aug. 2012, Invited

  
• 膜骨格の仕切りによる細胞膜の組織化：高速１分子追跡による研究

  藤原敬宏

  第4回JSBi応用システムバイオロジー研究会 「細胞環境の測定とモデリング」ワークショップ、神戸, 07 Nov. 2011, JSBi応用システムバイオロジー研究会, Invited

  
• Hop diffusion of membrane lipids and proteins in the plasma membrane as directly observed by high-speed single fluorescent-molecule tracking

  T. Fujiwara

  13th International Membrane Research Forum, Kyoto, 29 Jan. 2010, Invited

  
• Compartmentalization of the plasma membrane by actin-based membrane skeleton as revealed by high-speed single fluorescent-molecule tracking

  T. Fujiwara; S. Takeuchi; Y. Nagai; K. Hanaka; K. Iwasawa; K. Suzuki; A. Kusumi

  第82回日本生化学会大会 シンポジウム1S12a, 21 Oct. 2009, Invited

  
• 超高速1蛍光分子追跡法による、コンパートメント化された細胞膜内でのリン脂質のホップ拡散の検出

  藤原敬宏

  第61回日本細胞生物学会大会 ランチョンセミナー1、名古屋, 02 Jun. 2009, 株式会社ニコン, Invited

  
• 蛍光分子追跡法による，コンパートメント化された細胞膜内でのリン脂質のホップ拡散の検出

  藤原敬宏

  第61 回日本細胞生物学会大会ランチョンセミナー1（2009.6.2 名古屋）, 2009

  
• Super-speed single fluorescent-molecule tracking revealed hop diffusion of a phospholipid in the compartmentalized plasma membrane

  T. Fujiwara

  第46回日本生物物理学会年会 シンポジウム2S2 細胞膜とアクチン骨格との相互作用：生物物理のアプローチ、福岡, 04 Dec. 2008, Invited

  
• Compartmentalization of the plasma membrane by the membrane skeleton: high-speed single-molecule tracking study

  T. Fujiwara; A. Kusumi

  The 9th Asia-Pacific Microscopy Conference (APMC9), Jeju, Korea, 05 Nov. 2008, Invited

  
• Super-speed single fluorescent-molecule tracking revealed hop diffusion of a phospholipid in the \ncompartmentalized plasma membrane

  藤原敬宏

  第46回日本生物物理学会年会シンポジウム2S2「細胞膜とアクチン 骨格との相互作用：生物物理のアプローチ」（2008.12.3-5．福岡）, 2008

  
• Compartmentalization of the plasma membrane by the membrane skeleton : high-speed \nsingle-molecule tracking study

  T. Fujiwara; A. Kusumi

  The 9th Asia-Pacific Microscopy Conference (2008.11.2-7. Jeju, Korea), 2008

  
• Compartmentalized movement of lipids in the cell membrane as revealed by single molecule techniques

  T. Fujiwara

  NCBS-ICORP-iCeMS Symposium on Spatial and Temporal Mapping of Membrane Molecules, National Centre for Biological Sciences, Bangalore, India, 20 Nov. 2007, Invited

  
• 一分子法で語る細胞膜のダイナミクス

  藤原敬宏

  日本生化学会関東支部講演会「手法で語る生体膜－生体膜の謎解きのために－」、東京, 14 Jul. 2007, Invited

  
• １分子法による生体膜分子の拡散運動の可視化

  藤原敬宏

  日本物理学会2007春季大会 領域12シンポジウム「粒子の拡散と媒質の構造」、鹿児島, 20 Mar. 2007, Invited

  
• Corralling of phospholipids and transmembrane proteins by “fences” and “pickets” in the cell membrane : single-molecule tracking study.

  T. Fujiwara; K. Iwasawa; K. Ritchie; K. Murase; Y. Umemura; H. Yamashita; K. Suzuki; A. Kusumi

  Fifth East Asian Biophysics Symposium & Forty-Fourth Annual Meeting of the Biophysical Society of Japan, Okinawa（Japan）2006.11.12－16, 12 Nov. 2006

  
• Compartmentalized movement of lipids in the cell membrane as revealed by single molecule techniques

  T. Fujiwara; A. Kusumi

  The 6th Congress of the Federation of Asian and Oceanian Physiological Societies, Seoul, Korea, 18 Oct. 2006, Invited

  
• Regulation mechanism for assembly of adaptor protein AP2 molecules in clathrin-coated pits as studied by single fluorophore video microscopy

  T. Fujiwara

  The 16th International Microscopy Congress, Sapporo, 04 Sep. 2006, Invited

  
• Revealing cell membrane organization using single-molecule tracking techniques

  T. Fujiwara

  Mini Symposium on “New Trends in Bioscience”, POSTECH, Pohang, Korea, 20 Jun. 2006, Invited

  
• Assembly mechanism for adaptor protein AP2 molecules in clathrin-coated pits as studied by single fluorescent molecule video imaging

  T. Fujiwara; N. Morone; E. Kajikawa; J. Keen; and A

  20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, Kyoto, 19 Jun. 2006

  
• Revealing cellular signaling nanosystems using single-molecule tracking

  T. Fujiwara; A. Kusumi

  Focus on Microscopy 2006, Perth, Australia, 09 Apr. 2006, Invited

  
• Hop diffusion of transmembrane proteins and lipids in various cell types as studied by single molecule techniques

  T. Fujiwara

  9th International Membrane Research Forum, Kyoto, 17 Mar. 2006, Invited

  
• １分子追跡による、細胞膜のシグナル変換機構の研究

  藤原敬宏

  第2回インビトロジェン・シンポジウム「バイオサイエンスの最先端」、湘南国際村, 02 Sep. 2005, Invited

  
• Formation mechanism of clathrin-coated pit as studied by recruitment of single AP2 molecules

  T. Fujiwara; N. Morone; E. Kajikawa; J. H. Keen; A. Kusumi

  The 58th Annual Meeting of the Japan Society for Cell Biology, Omiya, 17 Jun. 2005, Invited

  
• 1 分子蛍光法による、クラスリン被覆ピットアダプター分子AP2 の集合過程の可視化解析

  藤原敬宏; 諸根信弘; 梶川絵理子; 小林 剛; James H. Keen; 楠見明弘

  日本顕微鏡学会第61回学術講演会 シンポジウムBS06「1 分子GFP イメージング」、つくば, 01 Jun. 2005, Invited

  
• Regulation mechanism for the assembly of adaptor protein AP2 molecules in clathrin-coated pits as studied by single fluorescent molecule video imaging

  T. Fujiwara; N. Morone; H. Mizoguchi; E. Kajikawa; C. Ohshima; T. Kobayashi; J. H. Keen; A. Kusumi

  American Biophysical Society 49th Annual Meeting, Long Beach, USA, 13 Feb. 2005, Invited

  
• Formation mechanisms of clathrin-coated pit as studied by recruitment of single AP2 molecules

  T. Fujiwara; N. Morone; E. Kajikawa; J.H. Keen; A. Kususmi

  第58回日本細胞生物学会大会 （2005.6.17. 大宮）, 2005

  
• Continuous rapid in/out’s of the adaptor protein AP2 by an antenna mechanism in clathrin-coated pit formation as revealed by single fluorophore molecule video microscopy

  T. Fujiwara; N. Morone; H. Mizoguchi; E. Kajikawa; C. Ohshima; T. Kobayashi; J. H. Keen; A. Kusumi

  The American Society for Cell Biology 44th Annual Meeting, Washington, DC, USA, 07 Dec. 2004, Invited

  
• Regulation mechanism for the formation of clathrin-coated pits as revealed by single fluorescent molecule video imaging of adaptor protein AP2

  T. Fujiwara

  8th International Membrane Research Forum, Nagoya, 23 Nov. 2004, Invited

  
• 細胞膜上のリン脂質の拡散運動遅延メカニズム：アンカード膜タンパク質ピケットモデル

  藤原敬宏; 楠見明弘

  日本膜学会第26回年会 ミニシンポジウム「細胞膜脂質のマイクロドメイン構造とその機能」、東京, 20 May 2004, Invited

  
• Continuous rapid exchange of the adaptor protein AP2 by an antenna mechanism in clathrin-coated pit formation

  T. Fujiwara

  Biology Department Seminar: Single molecule microscopy of plasma membrane dynamics, Johns Hopkins University, Baltimore, USA, 18 Feb. 2004, Invited

  
• Regulation mechanism for the assembly of adaptor protein AP2 molecules in clathrin-coated pits as studied by single fluorophore video microscopy

  T. Fujiwara; H. Mizoguchi; E. Kajikawa; N. Morone; C. Ohshima; T. Kobayashi; J. H. Keen; A. Kusumi

  JSPS-DST Asia Academic Seminar, NCBS Japan Workshop on Single Molecule Biophysics, National Centre for Biological Sciences, Tata Institute of Fundamental Research, GKVK Campus, Bangalore, India, 05 Jan. 2004, Invited

  
• Dynamic and cooperative assembly of clathrin-coated pits as revealed by single fluorophore video observations of AP2 and receptor molecules

  T. Fujiwara

  The American Society for Cell Biology 42nd Annual Meeting, San Francisco, USA, 16 Dec. 2002, Invited

  
• Dynamic and cooperative assembly of clathrin-coated pits as revealed by single fluorophore video observations of AP2 and receptor molecules

  T. Fujiwara

  6th International Membrane Research Forum, Nagoya, 04 Nov. 2002, Invited

  
• 1分子蛍光追跡による、AP2とトランスフェリン受容体のクラスリン被覆ピットへの集合機構の解析

  藤原敬宏

  第55回日本細胞生物学会・第35回日本発生生物学会 合同大会 オリンパスランチョンセミナー、横浜, 22 May 2002, オリンパス, Invited

  
• 全反射蛍光顕微鏡のクラスリン被覆ピット形成への応用

  藤原敬宏

  第1回オリンパスTIRFM全反射蛍光顕微鏡ワークショップ、東京, 15 Mar. 2002, オリンパス, Invited

  
• Regulation mechanism for the assembly of receptors and adaptor molecules in clathrin-coated pits as studied by single fluorophore video microscopy

  T. Fujiwara

  Membrane organization, domain formation and localized signaling in live cells: The single molecule approach, The Scripps Research Institute, USA, 28 Feb. 2002, Invited

  
• Regulation mechanism for the assembly of receptors and adaptor molecules in clathrin-coated pits as studied by single fluorophore video microscopy

  T. Fujiwara; R. Iino; I. Gaidarov; J. H. Keen; A. Kusumi

  American Biophysical Society 46th Annual Meeting, San Francisco, USA, 24 Feb. 2002, Invited

  
• 細胞膜の組織化と機能：１分子法による研究

  藤原敬宏; 小林剛; 楠見明弘

  第54回コロイドおよび界面化学討論会 (日本化学会) 依頼講演 3D08、日野, 18 Sep. 2001, Invited

  
• Double-compartmentalization of the movement of phospholipids in the plasma membrane through anchored-protein pickets

  T. Fujiwara

  5th International Membrane Research Forum, Nagoya, 04 Jun. 2001, Invited

  
• 膜骨格フェンスとアンカード膜タンパク質ピケットによる、細胞膜のコンパートメント化：1分子可視化による研究

  藤原敬宏; Ritchie Ken; 楠見明弘

  日本電子顕微鏡学会第57回学術講演会 シンポジウム BS09.「1分子技術による生細胞中の超分子システム研究の展開」、福岡, 10 May 2001, Invited

  
• 細胞膜におけるリン脂質のコンパートメント化された運動：アンカード膜タンパク質ピケットモデル

  藤原敬宏; Ritchie Kenneth; 楠見明弘

  第73回日本生化学会大会 シンポジウム41 「動態・情報の1分子イメージング」、横浜, 13 Oct. 2000, Invited

  
• Compartmentalized movement of phospholipids in the plasma membrane: anchored-protein picket model

  T. Fujiwara; A. Kusumi

  American Biophysical Society 44th Annual Meeting, New Orleans, USA, 15 Feb. 2000, Invited

  
• 細胞膜におけるリン脂質のホップ拡散：一分子レベルでの観察

  藤原敬宏; 楠見明弘

  第71回日本生化学会大会 シンポジウム 「見る、操作する生化学」、名古屋, 15 Oct. 1998, Invited

  

External funds: Kakenhi

• Unraveling plasma membrane organization based on cooperative regulation by heterogeneous cytoskeletal filaments

  Grant-in-Aid for Scientific Research (B)

  Basic Section 28040:Nanobioscience-related

  Kyoto University

  藤原 敬宏

  From 01 Apr. 2024, To 31 Mar. 2027, Granted

  細胞膜;細胞骨格;高速1蛍光分子追跡;生細胞超解像観察

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24K01310/
• Development of cross-scale technologies for investigating correlations of structures, dynamics, and functions of intracellular molecules

  Grant-in-Aid for Transformative Research Areas (A)

  Transformative Research Areas, Section (III)

  Tohoku University

  水上 進

  From 10 Sep. 2021, To 31 Mar. 2026, Granted

  クロススケール;マルチ計測;1分子超解像イメージング;細胞内標識

  https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-21H05252/
• Revealing the nano-liquid signaling platform by developing the ultrafast single-molecule super-resolution method

  Grant-in-Aid for Scientific Research (A)

  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields

  Okinawa Institute of Science and Technology Graduate University

  楠見 明弘

  From 05 Apr. 2021, To 31 Mar. 2025, Granted

  超速超解像1分子イメジング;アクチン膜骨格;液状ナノ構造;過渡的形成;シグナル基盤

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H04772/
• Direct single-molecule observation of the elementary process of molecular complex formation regulated by the compartmentalized plasma membrane

  Grant-in-Aid for Scientific Research (B)

  Basic Section 28040:Nanobioscience-related

  Kyoto University

  Takahiro Fujiwara

  From 01 Apr. 2020, To 31 Mar. 2023, Project Closed

  1分子観察;超解像観察;細胞膜;アクチン膜骨格;閉じ込め効果

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H02585/
• Development of high-speed super-resolution membrane skeleton elasticity mapping and its application to cell motility analysis

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 28:Nano/micro science and related fields

  Kyoto University

  Takahiro Fujiwara

  From 29 Jun. 2018, To 31 Mar. 2021, Project Closed

  超解像蛍光顕微鏡法;アクチン膜骨格;１蛍光分子追跡;バネ弾性;1蛍光分子追跡

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18K19001/
• Signal transduction by transient molecular complexes and its regulation by actin membrane skeleton: single-molecule tracking study

  Grant-in-Aid for Scientific Research (S)

  Okinawa Institute of Science and Technology Graduate University;Kyoto University

  Akihiro Kusumi

  From 31 May 2016, To 31 Mar. 2021, Project Closed

  超長時間１分子イメジング;超高速１分子PALM同時イメジング;過渡的シグナル分子複合体;アクチン膜骨格;インテグリン;LATベシクル;Gタンパク質共役受容体ダイマー;過渡的分子複合体;細胞膜;シグナル複合体;シグナル変換

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16H06386/
• Diamond NVC for protein analysis

  Grant-in-Aid for Challenging Exploratory Research

  Kyoto University

  Hidehito Tochio

  From 01 Apr. 2016, To 31 Mar. 2017, Project Closed

  ダイアモンドNVC;ダイヤモンド;NVC;１分子計測;蛍光イメージング;磁気共鳴

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K14679/
• Dynamic focal adhesion molecular islands as revealed by super-spatiotemporal-resolution single fluorescent-molecule localization microscopy

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Takahiro Fujiwara

  From 01 Apr. 2016, To 31 Mar. 2019, Project Closed

  接着斑;アクチン骨格;拡散運動;1分子計測;超解像;１分子計測

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16H04775/
• １分子超解像観察による、一次シリア基底部の分子選択的拡散障壁機構の解明

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Biological Sciences

  Kyoto University

  藤原 敬宏

  From 01 Apr. 2015, To 31 Mar. 2017, Project Closed

  一次シリア;拡散運動;１分子計測;３次元追跡;超解像;細胞膜

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-15H01212/
• Site-specific modification of respiratory complex I for single molecule analysis

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Hideto Miyoshi

  From 01 Apr. 2014, To 31 Mar. 2017, Project Closed

  生物制御化学;呼吸鎖複合体-I;化学修飾;ユビキノン;ミトコンドリア呼吸鎖;トシル化学;複合体-I;ミトコンドリア;呼吸鎖;呼吸鎖酵素

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26292060/
• Spatio-temporal analysis of extracellular ATP signaling of pancreatic islets

  Grant-in-Aid for Scientific Research (B)

  Kyoto University

  Hiromi Imamura

  From 01 Apr. 2014, To 31 Mar. 2017, Project Closed

  ATP;バイオセンサー;蛍光タンパク質;ブリン受容体;インスリン;プリン受容体;ライブイメージング;生物発光;BRET;ATPシグナル;イメージング

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26291027/
• 動的少数分子複合体ユニット機構：３次元１分子超局在顕微鏡による解明

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Biological Sciences

  Kyoto University

  藤原 敬宏

  From 01 Apr. 2014, To 31 Mar. 2016, Project Closed

  １分子計測;３次元追跡;超解像;細胞膜;接着構造

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-26115707/
• Analysis of moisturization mechanism of stratum corneusm

  Grant-in-Aid for Scientific Research (C)

  Institute of Physical and Chemical Research

  Takeshi Matsui

  From 01 Apr. 2013, To 31 Mar. 2018, Project Closed

  皮膚;表皮;角質層;バリア;細胞死;イメージング;ケラチン;epidermis;stratum granulosum;barrier;differentiation;skin;保湿;細胞;アトピー性皮膚炎;プロテアーゼ;バリアー

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25461667/
• 一次シリア基底部における選択的拡散障壁・分子フィルター機構の解明

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Biological Sciences

  Kyoto University

  藤原 敬宏

  From 01 Apr. 2013, To 31 Mar. 2015, Project Closed

  １分子計測;細胞膜;一次シリア;拡散運動;1分子計測

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-25113515/
• 動的カドヘリン複合体の機能：３次元１分子超追跡法の開発による解明

  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Biological Sciences

  Kyoto University

  藤原 敬宏

  From 01 Apr. 2012, To 31 Mar. 2014, Project Closed

  1分子計測;3次元追跡;細胞膜;細胞間接着分子;１分子計測;３次元追跡

  https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-24115510/
• Development of single-molecule tracking using fluorescent silicon nanoparticles and its application to signaling mechanisms in clathrin-coated pits

  Grant-in-Aid for Scientific Research (C)

  Kyoto University

  Takahiro FUJIWARA

  From 01 Apr. 2009, To 31 Mar. 2012, Project Closed

  1分子追跡;蛍光プローブ;細胞膜;受容体;クラスリン被覆ピット;シグナル伝達

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570167/