Basic Information

Academic Degree

• (The University of Tokyo)
• (The University of Tokyo)

Research History

• From Apr. 2018, To Present

  Kyoto University, Graduate School of Biostudies, Assistant Professor
• From Apr. 2012, To Mar. 2018

  RIKEN, Brain Science Institute, Researcher

Profile

• Profile

  コツコツと地道に研究しています。

Language of Instruction

• Japanese
• English

ID,URL

• J-Global ID

  201301060869537385

researchmap URL

• https://researchmap.jp/aki-miya

Research

Research Topics, Overview of the research

• Research Topics

  Elucidating the regulatory machinery of neuronal network by using

• Overview of the research

  Synaptic plasticity has a fundamental roles on learning and memory formation. Neuron has a lot of small membranous protrusion called spine that receives information from a single axon. The shape and size of spine controls synaptic plasticity. Thus I want to visualize precise distributions and interactions of functional proteins that control the structure of spine by using a novel super resolution microscopy, IRIS, and clarify the regulatory roles of synaptic plasticity.

Research Interests

• Antibody engineering
• Super resolution microscopy
• imaging
• apoptosis
• calcium

Research Areas

• Life sciences, Morphology, anatomy
• Life sciences, Neuroscience - general

Papers

• 

  Protocol to generate fast-dissociating recombinant antibody fragments for multiplexed super-resolution microscopy

  Qianli Zhang; Akitoshi Miyamoto; Naoki Watanabe

  STAR Protocols, Sep. 2023, Peer-reviewed, Invited

  Access this paper :

  
• 

  Engineered fast-dissociating antibody fragments for multiplexed super-resolution microscopy

  Qianli Zhang; Akitoshi Miyamoto; Shin Watanabe; Takao Arimori; Masanori Sakai; Madoka Tomisaki; Tai Kiuchi; Junichi Takagi; Naoki Watanabe

  Cell Reports Methods, Oct. 2022, Peer-reviewed

  Access this paper :

  
• Tuberous Sclerosis Complex (TSC) Inactivation Increases Neuronal Network Activity by Enhancing Ca²⁺ Influx via L-Type Ca²⁺ Channels

  Chihiro Hisatsune; Tadayuki Shimada; Akitoshi Miyamoto; Amy Lee; Kanato Yamagata

  The Journal of Neuroscience, 29 Sep. 2021, Peer-reviewed

  
• Bidirectional Control of Synaptic GABA(A)R Clustering by Glutamate and Calcium

  Hiroko Bannai; Fumihiro Niwa; Mark W. Sherwood; Amulya Nidhi Shrivastava; Misa Arizono; Akitoshi Miyamoto; Kotomi Sugiura; Sabine Levi; Antoine Triller; Katsuhiko Mikoshiba

  CELL REPORTS, Dec. 2015, Peer-reviewed

  
• 3PT206 Regulation of GABAergic synapses by IP_3/Ca^<2+> signaling(The 50th Annual Meeting of the Biophysical Society of Japan)

  Bannai Hiroko; Niwa Fumihiro; Sherwood Mark W.; Arizono Misa; Miyamoto Akitoshi; Sugiura Kotomi; Levi Sabine; Triller Antoine; Mikoshiba Katsuhiko

  Seibutsu Butsuri, 2012

  
• Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs

  Toru Matsu-ura; Hideki Shirakawa; Kenichi G; N. Suzuki; Akitoshi Miyamoto; Kotomi Sugiura; Takayuki Michikawa; Akihiro Kusumi; Katsuhiko Mikoshiba

  Scientific Reports, 2019, Peer-reviewed

  
• A novel multi lines analysis tool of Ca2+ dynamics reveals the nonuniformity of Ca2+ propagation.

  Akitoshi Miyamoto; Katsuhiko Mikoshiba

  Cell Calcium, 2019, Peer-reviewed, Lead author, Corresponding author

  
• A novel gain-of-function mutation in the ITPR1 suppressor domain causes spinocerebellar ataxia with altered Ca2+ signal patterns

  Jillian P. Casey; Taisei Hirouchi; Chihiro Hisatsune; Bryan Lynch; Raymond Murphy; Aimee M. Dunne; Akitoshi Miyamoto; Sean Ennis; Nick van der Spek; Bronagh O'Hici; Katsuhiko Mikoshiba; Sally Ann Lynch

  JOURNAL OF NEUROLOGY, Jul. 2017, Peer-reviewed

  
• Probes for manipulating and monitoring IP3.

  Akitoshi Miyamoto; Katsuhiko Mikoshiba

  Cell Calcium, 2016, Peer-reviewed, Invited, Lead author

  
• Development of a convenient and supersensitive high-throughput screening system for genetically encoded fluorescent probes of small molecules using a confocal microscope.

  Akitoshi Miyamoto; Kotomi Sugiura; Katsuhiko Mikoshiba

  Cell Calcium, 2016, Peer-reviewed, Lead author

  
• Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by disulfide reductase ERdj5

  Ryo Ushioda; Akitoshi Miyamoto; Michio Inoue; Satoshi Watanabe; Masaki Okumura; Ken-ichi Maegawa; Kaiku Uegaki; Shohei Fujii; Yasuko Fukuda; Masataka Umitsu; Junichi Takagi; Kenji Inaba; Katsuhiko Mikoshiba; Kazuhiro Nagata

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Oct. 2016, Peer-reviewed, Lead author

  
• Differential calcium-dependent control of synaptic GABAA receptor clustering by ionotropic and metabotropic glutamate receptors

  Hiroko Bannai; Fumihiro Niwa; Mark W Sherwood; Amulya Nidhi Shrivastava; Misa Arizono; Akitoshi Miyamoto; Kotomi Sugiura; Sabine Lévi; Antoine Triller; Katsuhiko Mikoshiba

  Cell Reports, 2015, Peer-reviewed

  
• Apoptosis induction-related cytosolic calcium responses revealed by the dual FRET imaging of calcium signals and caspase-3 activation in a single cell

  Akitoshi Miyamoto; Hiroshi Miyauchi; Takako Kogure; Atsushi Miyawaki; Takayuki Michikawa; Katsuhiko Mikoshiba

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Apr. 2015, Peer-reviewed, Lead author

  
• Optimal microscopic systems for long-term imaging of intracellular calcium using a ratiometric genetically-encoded calcium indicator

  Akitoshi Miyamoto; Hiroko Bannai; Takayuki Michikawa; Katsuhiko Mikoshiba

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 2013, Peer-reviewed, Lead author

  
• Receptor-Selective Diffusion Barrier Enhances Sensitivity of Astrocytic Processes to Metabotropic Glutamate Receptor Stimulation

  Misa Arizono; Hiroko Bannai; Kyoko Nakamura; Fumihiro Niwa; Masahiro Enomoto; Toru Matsu-ura; Akitoshi Miyamoto; Mark W. Sherwood; Takeshi Nakamura; Katsuhiko Mikoshiba

  SCIENCE SIGNALING, Apr. 2012, Peer-reviewed

  
• Mechanistic basis of bell-shaped dependence of inositol 1,4,5-trisphosphate receptor gating on cytosolic calcium

  Tadashi Shinohara; Takayuki Michikawa; Masahiro Enomoto; Jun-Ichi Goto; Miwako Iwai; Toru Matsu-ura; Haruka Yamazaki; Akitoshi Miyamoto; Akio Suzuki; Katsuhiko Mikoshiba

  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Sep. 2011, Peer-reviewed

  

Misc.

• Receptor-selective diffusion barrier enhances sensitivity of astrocytic processes to metabotropic glutamate receptor stimulation

  M. Arizono; H. Bannai; K. Nakamura; F. Niwa; M. Enomoto; T. Matsu-ura; A. Miyamoto; M. W. Sherwood; T. Nakamura; K. Mikoshiba

  JOURNAL OF NEUROCHEMISTRY, Oct. 2012

  
• Mechanistic basis of the bell-shaped dependence of inositol 1,4,5-trisphosphate receptor gating on cytosolic calcium

  Takayuki Michikawa; Tadashi Shinohara; Masahiro Enomoto; Jun-Ichi Goto; Miwako Iwai; Toru Matsu-ura; Haruka Yamazaki; Akitoshi Miyamoto; Akio Suzuki; Katsuhiko Mikoshiba

  NEUROSCIENCE RESEARCH, 2011

  
• Astrocyte processes exhibit confined mGluR5 diffusion to maintain high mGluR5 density and high sensitivity to mGluR agonist

  Misa Arizono; Takeshi Nakamura; Kyoko Nakamura; Masahiro Enomoto; Akitoshi Miyamoto; Hiroko Bannai; Katsuhiko Mikoshiba

  NEUROSCIENCE RESEARCH, 2010

  

Books and Other Publications

• Precision Medicine 「病理/組織画像評価法の潮流」

  宮本章歳; 渡邊直樹, 多重高密度超解像顕微鏡IRISによる多分子複合体マッピングの病理診断への応用

  Sep. 2023

  

Industrial Property Rights

• 特願2022-126485, 多重抗原検出用抗体

  張千里; 宮本章歳; 渡邊直樹

  

Awards

• 11 Jan. 2016

  Glial Assembly: a New Regulatory Machinery of Brain Function and Disorders, YoungGlia Platinum Award

External funds: Kakenhi

• 革新的な超解像顕微鏡を用いた神経伝達機構の解明

  Grant-in-Aid for Scientific Research (C)

  Basic Section 46010:Neuroscience-general-related

  Kyoto University

  宮本 章歳

  From 01 Apr. 2023, To 31 Mar. 2026, Granted

  超解像顕微鏡;神経伝達

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K05963/