Basic Information

Affiliated programs (koza)

• Graduate School of Science, 生物科学専攻　 生体分子情報学講座, 助教

Professional Memberships

• 日本生化学会
• 日本結晶学会

Academic Degree

• 修士（理学）(東京工業大学)
• 博士（理学）(東京工業大学)

Language of Instruction

• English

ID,URL

• J-Global ID

  200901086359209193

researchmap URL

• https://researchmap.jp/read0042646

Research

Research Topics, Overview of the research

• Research Topics

  Structural Biology, Protein Crystallography

• Overview of the research

  The structural biology about the relationships between protein structures and their functions and properties based on the crystal structures determined by X-ray analyses is studied. The main research themes are elucidation of the enzymatic reaction mechanism and the adaptation strategy of proteins from thermophilic or cold-adapted bacteria.
  Structural Study for Thermostability of Protein Molecules
  

Research Interests

• タンパク質結晶学
• Protein Crystallography

Research Areas

• Life sciences, Biophysics

Papers

• The crystal structure of maleylacetate reductase from Rhizobium sp strain MTP-10005 provides insights into the reaction mechanism of enzymes in its original family

  Tomomi Fujii; Ai Sato; Yuko Okamoto; Takae Yamauchi; Shiro Kato; Masahiro Yoshida; Tadao Oikawa; Yasuo Hata

  PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, Aug. 2016, Peer-reviewed

  
• Crystallographic studies of aspartate racemase from Lactobacillus sakei NBRC 15893

  Tomomi Fujii; Takae Yamauchi; Makoto Ishiyama; Yoshitaka Gogami; Tadao Oikawa; Yasuo Hata

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Aug. 2015, Peer-reviewed

  
• Crystal Structure Analysis of GraE Protein from the Gene Cluster of γ-Resorcylate Catabolic System

  T. Yamauchi; T. Fujii; T. Oikawa; Y. Hata

  SPring-8 / SACLA Research Report, 2015, Peer-reviewed

  
• Crystal Structures of Bacterial Flavin Reductase GraD and Its Complex with NADH

  Yamauchi, T; Fujii, T; Yoshida, M; Oikawa, T; Hata, Y

  Acta Cryst., 2014, Peer-reviewed

  
• Crystal Structure Analyses of Oxygenase Component of Resorcinol Hydroxylase

  Fujii, T; Kobayashi, K; Yamauchi, T; Yoshida, M; Oikawa, T; Hata, Y

  Acta Cryst., 2014, Peer-reviewed

  
• Structural Features and Low-Temperature Adaptation of Aspartate Racemase

  Hata, Y; Fujii, T; Yamauchi, T; Gogami, Y; Oikawa, T

  Acta Cryst., 2014, Peer-reviewed

  
• Crystal Structure of Flavin Reductase from Rhizobium sp. strain MTP-10005

  Hata, Y; Yamauchi, T; Fujii, T; Yoshida, M; Oikawa, T

  Abstract of AsCA’13, 2013, Peer-reviewed

  
• Structural Studies on Thermostability of Aspartate Racemase

  Hata, Y; Fujii, T; Yamauchi, T; Gogami, Y; Oikawa, T

  Abstract of AsCA 12, 2012, Peer-reviewed

  
• Crystal Structure of Aspartate Rasemase from Lactobacillus sakei NBRC-15893

  Hata, Y; Fujii, T; Ishiyama, M; Yamauchi, T; Gogami, Y; Oikawa, T

  Acta Cryst., 2011, Peer-reviewed

  
• Crystal Structure of Flavin Reductase from Rhizobium sp. Strain MTP-10005

  Yamauchi, T; Fujii, T; Yoshida, M; Oikawa, T; Hata, Y

  Acta Cryst., 2011, Peer-reviewed

  
• Structure and Reaction Mechanism of Maleylacetate Reductase.

  Hata Y; Fujii T; Yoshida M; Oikawa T

  Abstract of Pacifichem 2010 Honolulu., 2010, Peer-reviewed

  
• Crystal Structure Analysis of the Oxygenase Component (GraA) of a Resorcinol Hydroxylase.

  Hata Y; Fujii T; Kobayashi K; Yoshida M; Oikawa T

  Abstract of AsCA2010 Busan., 2010, Peer-reviewed

  
• Structure of Maleylacetate Reductase from Rhizobium sp. strain MTP-10005

  Hata Y; Fujii T; Yoshida M; Oikawa T

  Abstract of AsCA'9 Beijing, 2009, Peer-reviewed

  
• Crystallization and preliminary X-ray diffraction studies of maleylacetate reductase from Rhizobium sp strain MTP-10005

  Tomomi Fujii; Yuko Goda; Masahiro Yoshida; Tadao Oikawa; Yasuo Hata

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Aug. 2008, Peer-reviewed

  
• Crystallization and preliminary X-ray diffraction studies of tetrameric malate dehydrogenase from the novel Antarctic psychrophile Flavobacterium frigidimaris KUC-1

  Tomomi Fujii; Tadao Oikawa; Ikuo Muraoka; Kenji Soda; Yasuo Hata

  ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, Nov. 2007, Peer-reviewed

  
• Structure of the carboxypeptidase Y inhibitor I-C in complex with the cognate proteinase reveals a novel mode of the proteinase-protein inhibitor interaction

  J Mima; M Hayashida; T Fujii; Y Narita; R Hayashi; M Ueda; Y Hata

  JOURNAL OF MOLECULAR BIOLOGY, Mar. 2005, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of carboxypeptidase Y inhibitor I-C complexed with the cognate proteinase

  J Mima; M Hayashida; T Fujii; Y Hata; R Hayashi; M Ueda

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Sep. 2004, Peer-reviewed

  
• Structures of two lectins from the roots of pokeweed (Phytolacca americana)

  T Fujii; M Hayashida; M Hamasu; M Ishiguro; Y Hata

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Apr. 2004, Peer-reviewed

  
• Similarity between protein-protein and protein-carbohydrate interactions, revealed by two crystal structures of lectins from the roots of pokeweed

  M Hayashida; T Fujii; M Hamasu; M Ishiguro; Y Hata

  JOURNAL OF MOLECULAR BIOLOGY, Nov. 2003, Peer-reviewed

  
• Crystallization and preliminary X-ray analysis of lectin C from the roots of pokeweed (Phytolacca americana)

  M Hayashida; T Fujii; M Hamasu; M Ishiguro; Y Hata

  ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Jul. 2003, Peer-reviewed

  
• Crystal structure of thermostable aspartase from Bacillus sp YM55-1: Structure-based exploration of functional sites in the aspartase family

  T Fujii; H Sakai; Y Kawata; Y Hata

  JOURNAL OF MOLECULAR BIOLOGY, May 2003, Peer-reviewed

  
• Structure of external aldimine of Escherichia coli CsdB, an IscS/NifS homolog: Implications for its specificity toward selenocysteine

  Hisaaki Mihara; Tomomi Fujii; Shin ichiro Kato; Tatsuo Kurihara; Yasuo Hata; Nobuyoshi Esaki

  Journal of Biochemistry, 2002, Peer-reviewed

  
• Crystal structures of the transposon Tn5-carried bleomycin resistance determinant uncomplexed and complexed with bleomycin

  M Maruyama; T Kumagai; Y Matoba; M Hayashida; T Fujii; Y Hata; M Sugiyama

  JOURNAL OF BIOLOGICAL CHEMISTRY, Mar. 2001, Peer-reviewed

  
• Structure of a NifS homologue: X-ray structure analysis of CsdB, an Escherichia coli counterpart of mammalian selenocysteine lyase

  T Fujii; M Maeda; H Mihara; T Kurihara; N Esaki; Y Hata

  BIOCHEMISTRY, Feb. 2000, Peer-reviewed

  
• A nifS-like gene, csdB, encodes an Escherichia coli counterpart of mammalian selenocysteine lyase - Gene cloning, purification, characterization and preliminary x-ray crystallographic studies

  H Mihara; M Maeda; T Fujii; T Kurihara; Y Hata; N Esaki

  JOURNAL OF BIOLOGICAL CHEMISTRY, May 1999, Peer-reviewed

  
• X-ray structure of a reaction intermediate of L-2-haloacid dehalogenase with L-2-chloropropionamide

  YF Li; Y Hata; T Fujii; T Kurihara; N Esaki

  JOURNAL OF BIOCHEMISTRY, Jul. 1998, Peer-reviewed

  
• Crystal structures of reaction intermediates of L-2-haloacid dehalogenase and implications for the reaction mechanism

  YF Li; Y Hata; T Fujii; T Hisano; M Nishihara; T Kurihara; N Esaki

  JOURNAL OF BIOLOGICAL CHEMISTRY, Jun. 1998, Peer-reviewed

  
• Conformational analysis of a fluorescent rotor, 6-(2,2-dicyanovinyl)-1-(2-hydroxyethyl)-1,2,3,4-tetrahydroquinoline

  Y Yamamoto; T Okajima; Y Fukazawa; T Fujii; Y Hata; S Sawada

  JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, Mar. 1998, Peer-reviewed

  
• Binding mode of CA074, a specific irreversible inhibitor, to bovine cathepsin B as determined by X-ray crystal analysis of the complex

  A Yamamoto; T Hara; K Tomoo; T Ishida; T Fujii; Y Hata; M Murata; K Kitamura

  JOURNAL OF BIOCHEMISTRY, May 1997, Peer-reviewed

  
• The crystal structure of zinc-containing ferredoxin from the thermoacidophilic archaeon Sulfolobus sp strain 7

  T Fujii; Y Hata; M Oozeki; H Moriyama; T Wakagi; N Tanaka; T Oshima

  BIOCHEMISTRY, Feb. 1997, Peer-reviewed

  
• Asymmetric cycloaddition of anthrone with N-substituted maleimides with C-2-chiral pyrrolidines

  K Tokioka; S Masuda; T Fujii; Y Hata; Y Yamamoto

  TETRAHEDRON-ASYMMETRY, Jan. 1997, Peer-reviewed

  
• Novel zinc-binding centre in thermoacidophilic archaeal ferredoxins

  T Fujii; Y Hata; T Wakagi; N Tanaka; T Oshima

  NATURE STRUCTURAL BIOLOGY, Oct. 1996, Peer-reviewed

  
• Molecular cloning, sequencing, and heterologous expression of a novel zinc-containing ferredoxin gene from a thermoacidophilic archaeon Sulfolobus sp strain 7

  T Wakagi; T Fujii; T Oshima

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Aug. 1996, Peer-reviewed

  
• Crystal structure of L-2-haloacid dehalogenase from Pseudomonas sp YL - An alpha/beta hydrolase structure that is different from the alpha/beta hydrolase fold

  T Hisano; Y Hata; T Fujii; JQ Liu; T Kurihara; N Esaki; K Soda

  JOURNAL OF BIOLOGICAL CHEMISTRY, Aug. 1996, Peer-reviewed

  
• Crystallization and preliminary X-ray crystallographic studies of L-2-haloacid dehalogenase from Pseudomonas sp. YL

  T Hisano; Y Hata; T Fujii; JQ Liu; T Kurihara; N Esaki; K Soda

  PROTEINS-STRUCTURE FUNCTION AND GENETICS, Apr. 1996, Peer-reviewed

  
• CRYSTAL-STRUCTURE OF THE UNLIGANDED ALKALINE PROTEASE FROM PSEUDOMONAS-AERUGINOSA IFO3080 AND ITS CONFORMATIONAL-CHANGES ON LIGAND-BINDING

  H MIYATAKE; Y HATA; T FUJII; K HAMADA; K MORIHARA; Y KATSUBE

  JOURNAL OF BIOCHEMISTRY, Sep. 1995, Peer-reviewed

  
• ALTERNATIVE FORM OF THE DICLUSTER FERREDOXIN FROM THE THERMOACIDOPHILIC ARCHAEON, SULFOLOBUS SP STRAIN-7

  T IWASAKI; T FUJII; T WAKAGI; T OSHIMA

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Jan. 1995, Peer-reviewed

  
• CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES ON SULFOLOBUS-ACIDOCALDARIUS FERREDOXIN

  T FUJII; H MORIYAMA; A TAKENAKA; N TANAKA; T WAKAGI; T OSHIMA

  JOURNAL OF BIOCHEMISTRY, Oct. 1991, Peer-reviewed

  

Misc.

• Thermococcus litoralis DSM 5473由来アスパラギナーゼのＸ線結晶構造

  藤井知実; 山内貴恵; 加藤志郎; 老川典夫; 畑安雄

  2017年度生命科学系学会合同年次大会, 2017

  
• Thermococcus litralis DSM 5473由来アスパラギナーゼのX線結晶構造解析

  山内貴恵; 藤井知実; 加藤志郎; 老川典夫; 畑安雄

  日本農芸化学会2016年度大会, Mar. 2016

  
• Thermococcus litoralis DSM 5473由来アスパラギナーゼの結晶構造解析

  藤井知実; 山内貴恵; 加藤志郎; 老川典夫; 畑安雄

  日本結晶学会平成28年度年会, 2016

  
• レゾルシノール水酸化酵素オキシゲナーゼコンポーネントの結晶構造

  藤井知実; 小林一隆; 山内貴恵; 吉田雅博; 老川典夫; 畑安雄

  第86回日本生化学会大会, 横浜, 2013

  
• レゾルシノール水酸化酵素オキシゲナーゼコンポーネントの結晶構造解析

  藤井知実; 小林一隆; 山内貴恵; 吉田雅博; 老川典夫; 畑安雄

  日本結晶学会平成２５年度年会, 熊本, 2013

  
• 微生物由来アスパラギン酸ラセマーゼのX線結晶構造解析

  山内貴恵; 藤井知実; 郷上佳孝; 老川典夫; 畑安雄

  日本農芸化学会2013年度大会, 2013

  
• 耐熱性アスパラギン酸ラセマーゼの結晶構造解析

  藤井知実; 細見卓; 山内貴恵; 郷上佳孝; 老川典夫; 畑安雄

  日本結晶学会年会2012 ,仙台, 26 Oct. 2012, Peer-reviewed

  
• 超好熱性古細菌Thermococcus litoralis DSM 5473由来アスパラギン酸ラセマーゼの結晶構造

  藤井知実; 細見卓; 山内貴恵; 郷上佳孝; 老川典夫; 畑安雄

  第85回日本生化学会大会, 福岡, 2012

  
• レゾルシノールヒドロキシラーゼの構造-機能解析

  山内貴恵; 小林一隆; 藤井知実; 吉田雅博; 老川典夫; 畑安雄

  第59回日本生化学会近畿支部例会, 2012

  
• Rhizobium sp. strain MTP-10005由来フラビン還元酵素のX線結晶構造解析

  山内貴恵; 藤井知実; 吉田雅博; 老川典夫; 畑安雄

  日本農芸化学会2012年度大会, 2012

  
• Crystal Structure of Maleylacetate Reductase

  Hata, Y; Fujii,T; Yoshida, M; Oikawa, T

  Pacific Protein Association Conference, 2011

  
• 好冷細菌Flavobacterium frigidimaris KUC-1由来マレートデヒドロゲナーゼの部位特異的変異導入及び分子動力学計算による低温適応機構の解析

  郷上佳孝; 茨木将; 藤井知実; 畑安雄; 老川典夫

  第2回近畿地区ビタミン懇話会, 神戸, 2011

  
• 根粒菌由来フラビン還元酵素のX線結晶構造解析

  山内貴恵; 藤井知実; 吉田雅博; 老川典夫; 畑安雄

  日本農芸化学会2011年度大会, 京都, 2011

  
• Structure of Maleylacetate Reductase from Rhizobium sp. strain MTP-10005

  Hata Y; Fujii T; Yoshida M; Oikawa T

  The 2010 International Chemical Congress of Pacific Basin Societies, 2010

  
• マレイル酢酸還元酵素のX線結晶構造解析

  藤井知実; 老川典夫; 畑安雄

  日本結晶学会 2010年度大会, 大阪, 2010

  
• マレイル酢酸還元酵素の結晶構造

  藤井知実; 山内貴恵; 老川典夫; 畑安雄

  日本生化学会 2010年度大会, 神戸, 2010

  
• A Psychrophilic Tetrameric Malate Dehydrogenase Has No Intersubunit Ion-pairs

  Hata Y; Fujii T; Oikawa T; Soda K

  Abstract of ABA Symposium, 2009, Peer-reviewed

  
• Crystal Structure of Tetrameric Malate Dehydrogenase from Antarctic Psychrophile

  Fujii T; Oikawa T; Muraoka I; Soda K; Hata Y

  XXI Congress and General Assembly of the International Union of Crystallography ,Osaka, 29 Aug. 2008, Peer-reviewed

  
• 「耐熱性アスパルターゼの結晶」

  畑安雄; 藤井知実

  国内特許公開, (特開2005-137218) 2005 年, 2005, Peer-reviewed

  
• アメリカヤマゴボウ由来レクチンの立体構造解析

  畑 安雄; 藤井 知実; 林田 稔; 水田 悟史; 濱洲 三加; 麻生 陽一; 石黒 正恒

  日本農芸化学会 ,, 26 Mar. 2002, Peer-reviewed

  
• 活性硫黄と活性型セレンの生成を担う酵素

  三原 久明; 栗原 達夫; 藤井 知実; 畑 安雄; 加藤 伸一郎; 吉村 徹; 江崎 信芳

  日本生化学会 ,京都, 25 Oct. 2001, Peer-reviewed

  
• 耐熱性アスパルターゼの結晶構造と機能部位探索

  藤井 知実; 酒井 久伸; 河田 康志; 畑 安雄

  日本結晶学会 ,名古屋, 03 Oct. 2001, Peer-reviewed

  
• 耐熱性アスパルターゼのX線結晶構造解析

  畑 安雄; 酒井 久伸; 藤井 知実; 河田 康志

  日本農芸化学会 ,, 02 Apr. 2000, Peer-reviewed

  
• 質量分析の活用：２ハロ酸デハロゲナーゼによる立体反転反応触媒の現場を見る

  江崎 信芳; 栗原 達夫; 李 永福; 市山 進; 藤井 知実; 畑 安雄; 小暮 佳史; 網澤 進; 左右田 健次

  日本生化学会 ,神奈川, 08 Oct. 1999, Peer-reviewed

  
• 大腸菌 NIFS 相同酵素の立体構造特性

  畑 安雄; 藤井 知実; 三原 久明; 栗原 達夫; 江崎信芳

  日本農芸化学会 ,, 31 Mar. 1999, Peer-reviewed

  
• 大腸菌由来NIFS相同酵素の結晶構造

  藤井 知実; 前田 匡穀; 三原 久明; 栗原 達夫; 江崎 信芳; 畑 安雄

  日本結晶学会 ,神奈川, 22 Nov. 1998, Peer-reviewed

  
• 大腸菌由来システインスルフィン酸デスルフィナーゼIIの結晶構造解析

  畑 安雄; 前田 匡毅; 藤井 知実; 三原 久明; 栗原 達夫; 江崎 信芳

  日本農芸化学会 ,, 02 Apr. 1998, Peer-reviewed

  
• 中間体構造に基づくL-2-ハロ酸デハロゲナーゼの触媒反応機構解析

  李 永福; 藤井 知実; 栗原 達夫; 江崎 信芳; 畑 安雄

  日本農芸化学会 ,, 01 Apr. 1998, Peer-reviewed

  
• L-2-ハロ酸デハロゲナーゼの反応機構解析-エステル中間体の構造

  畑 安雄; 李 永福; 藤井 知実; 栗原 達夫; 江崎 信芳

  日本結晶学会 ,茨木, 01 Nov. 1997, Peer-reviewed

  
• L-2ハロ酸デハロゲナーゼの立体構造と反応機能

  畑 安雄; 久野 玉雄; 藤井 知実; 栗原 達夫; 江崎 信芳; 左右田 健次

  日本農芸化学会 ,, 04 Apr. 1997, Peer-reviewed

  
• Pseudomonas sp. YL株由来L-2-ハロ酸デハロゲナーゼ：基質複合体のX線結晶解析

  李 永福; 西原 光洋; 藤井 知実; 栗原 達夫; 江崎 信芳; 畑 安雄

  日本農芸化学会 ,, 03 Apr. 1997, Peer-reviewed

  
• C2-キラルピロリジンによるアントロンと置換マレイミドとの不斉環状付加反応

  増田 聡史; 時岡 恭平; 藤井 知実; 畑 安雄; 山本 行男

  日本農芸化学会 ,, 01 Apr. 1997, Peer-reviewed

  
• L-2-ハロ酸デハロゲナーゼの結晶構造

  久野 玉雄; 畑 安雄; 藤井 知実; 栗原 達夫; 江崎 信芳; 左右田 健次

  日本結晶学会 ,名古屋, 07 Oct. 1995, Peer-reviewed

  
• 古細菌由来フェレドキシンの結晶構造解析

  藤井 知実; 畑 安雄; 大関 雅人; 森山 英明; 田中 信夫; 若木 高善; 大島 大郎

  日本結晶学会 ,大阪, 26 Nov. 1994, Peer-reviewed

  

Presentations

• 耐熱性アスパラギン酸ラセマーゼの結晶構造解析

  日本結晶学会年会2012, 26 Oct. 2012, 仙台

  
• Crystal Structure of Tetrameric Malate Dehydrogenase from Antarctic Psychrophile

  XXI Congress and General Assembly of the International Union of Crystallography, 29 Aug. 2008, Osaka

  
• アメリカヤマゴボウ由来レクチンの立体構造解析

  日本農芸化学会, 26 Mar. 2002

  
• 活性硫黄と活性型セレンの生成を担う酵素

  日本生化学会, 25 Oct. 2001, 京都

  
• 耐熱性アスパルターゼの結晶構造と機能部位探索

  日本結晶学会, 03 Oct. 2001, 名古屋

  
• 耐熱性アスパルターゼの結晶構造

  日本農芸化学会, 25 Mar. 2001

  
• 質量分析の活用：２ハロ酸デハロゲナーゼによる立体反転反応触媒の現場を見る

  日本生化学会, 08 Oct. 1999, 神奈川

  
• 大腸菌 NIFS 相同酵素の立体構造特性

  日本農芸化学会, 31 Mar. 1999

  
• 大腸菌由来NIFS相同酵素の結晶構造

  日本結晶学会, 22 Nov. 1998, 神奈川

  
• 大腸菌由来システインスルフィン酸デスルフィナーゼIIの結晶構造解析

  日本農芸化学会, 02 Apr. 1998

  
• 中間体構造に基づくL-2-ハロ酸デハロゲナーゼの触媒反応機構解析

  日本農芸化学会, 01 Apr. 1998

  
• L-2-ハロ酸デハロゲナーゼの反応機構解析-エステル中間体の構造

  日本結晶学会, 01 Nov. 1997, 茨木

  
• L-2ハロ酸デハロゲナーゼの立体構造と反応機能

  日本農芸化学会, 04 Apr. 1997

  
• Pseudomonas sp. YL株由来L-2-ハロ酸デハロゲナーゼ：基質複合体のX線結晶解析

  日本農芸化学会, 03 Apr. 1997

  
• C2-キラルピロリジンによるアントロンと置換マレイミドとの不斉環状付加反応

  日本農芸化学会, 01 Apr. 1997

  
• L-2-ハロ酸デハロゲナーゼの結晶構造

  日本結晶学会, 07 Oct. 1995, 名古屋

  
• 古細菌由来フェレドキシンの結晶構造解析

  日本結晶学会, 26 Nov. 1994, 大阪

  

External funds: Kakenhi

• アスパルターゼ機能発現部位における構造変化機構の解明

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  藤井 知実

  From 01 Apr. 2003, To 31 Mar. 2005, Project Closed

  構造生物学;X線結晶解析;アスパルターゼ;耐熱性;立体構造;結晶構造

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15780069/
• 高活性耐熱性アスパルターゼ反応機構の複合体結晶解析による解明

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  藤井 知実

  From 01 Apr. 2001, To 31 Mar. 2003, Project Closed

  構造生物学;X線結晶解析;アスパルターゼ;耐熱性;立体構造;結晶構造;局所構造比較

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13760070/
• 古細菌2-オキソ酸:フェレドキシン酸化還元酵素群の構造生物学研究

  Grant-in-Aid for Scientific Research on Priority Areas

  Biological Sciences

  Kyoto University

  畑 安雄

  From 01 Apr. 2001, To 31 Mar. 2003, Project Closed

  結晶構造;NifS;システインデスルフラーゼ;ビリドキサール5'-リン酸;PLP;セレノシステインリアーゼ;シッフ塩基;NifS相同タンパク質;PLP酵素;X線結晶構造解析;X線解析;立体構造

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13033020/
• 古細菌2-オキソ酸:フェレドキシン酸化還元酵素群の構造生物学研究

  Grant-in-Aid for Scientific Research on Priority Areas (A)

  Biological Sciences

  Kyoto University

  畑 安雄

  From 01 Apr. 1999, To 31 Mar. 2001, Project Closed

  NifS;NifS相同タンパク質;PLP酵素;X線結晶構造解;X線解析;結晶構造;立体構造;Nifs;Nifs相同タンパク質;リアーゼ;X線結晶解析;大腸菌酵素;二量体分子;α;β構造

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11169220/
• 硫黄供給酵素による脱離反応機構の構造生物学的研究

  Grant-in-Aid for Encouragement of Young Scientists (A)

  Kyoto University

  藤井 知実

  From 01 Apr. 1998, To 31 Mar. 2000, Project Closed

  構造生物学;X線結晶構造解析;ピリドキサールリン酸;システイン

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10760048/

Education

Teaching subject(s)

• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biomolecular Information IIB

  9094, Fall, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biomolecular Information IIA

  9093, Spring, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biomolecular Information ID

  9092, Fall, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biomolecular Information IID

  9096, Fall, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biomolecular Information IIC

  9095, Spring, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biomolecular Information IC

  9091, Spring, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biomolecular Information IB

  9090, Fall, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Seminar on Biomolecular Information IA

  9089, Spring, Graduate School of Science, 1
• From 01 Apr. 2023, To 31 Mar. 2024

  Advanced Course on Biological Science B

  9012, Fall, Graduate School of Science, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biomolecular Information IIA

  9093, Spring, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biomolecular Information IIB

  9094, Fall, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biomolecular Information ID

  9092, Fall, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biomolecular Information IA

  9089, Spring, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biomolecular Information IIC

  9095, Spring, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biomolecular Information IC

  9091, Spring, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biomolecular Information IID

  9096, Fall, Graduate School of Science, 1
• From 01 Apr. 2022, To 31 Mar. 2023

  Advanced Course on Biological Science B

  9012, Fall, Graduate School of Science, 2
• From 01 Apr. 2022, To 31 Mar. 2023

  Seminar on Biomolecular Information IB

  9090, Fall, Graduate School of Science, 1
• From Apr. 2017, To Mar. 2018

  Seminar on Biomolecular Information IA

  Spring, 理学研究科
• From Apr. 2017, To Mar. 2018

  Seminar on Biomolecular Information IB

  Fall, 理学研究科
• From Apr. 2017, To Mar. 2018

  Seminar on Biomolecular Information IC

  Spring, 理学研究科
• From Apr. 2017, To Mar. 2018

  Seminar on Biomolecular Information ID

  Fall, 理学研究科
• From Apr. 2017, To Mar. 2018

  Seminar on Biomolecular Information IIA

  Spring, 理学研究科
• From Apr. 2017, To Mar. 2018

  Seminar on Biomolecular Information IIB

  Fall, 理学研究科
• From Apr. 2017, To Mar. 2018

  Seminar on Biomolecular Information IIC

  Spring, 理学研究科
• From Apr. 2017, To Mar. 2018

  Seminar on Biomolecular Information IID

  Fall, 理学研究科
• From Apr. 2018, To Mar. 2019

  Seminar on Biomolecular Information IA

  Spring, 理学研究科
• From Apr. 2018, To Mar. 2019

  Seminar on Biomolecular Information IB

  Fall, 理学研究科
• From Apr. 2018, To Mar. 2019

  Seminar on Biomolecular Information IC

  Spring, 理学研究科
• From Apr. 2018, To Mar. 2019

  Seminar on Biomolecular Information ID

  Fall, 理学研究科
• From Apr. 2018, To Mar. 2019

  Seminar on Biomolecular Information IIA

  Spring, 理学研究科
• From Apr. 2018, To Mar. 2019

  Seminar on Biomolecular Information IIB

  Fall, 理学研究科
• From Apr. 2018, To Mar. 2019

  Seminar on Biomolecular Information IIC

  Spring, 理学研究科
• From Apr. 2018, To Mar. 2019

  Seminar on Biomolecular Information IID

  Fall, 理学研究科
• From Apr. 2019, To Mar. 2020

  Seminar on Biomolecular Information IA

  Spring, 理学研究科
• From Apr. 2019, To Mar. 2020

  Seminar on Biomolecular Information IB

  Fall, 理学研究科
• From Apr. 2019, To Mar. 2020

  Seminar on Biomolecular Information IC

  Spring, 理学研究科
• From Apr. 2019, To Mar. 2020

  Seminar on Biomolecular Information ID

  Fall, 理学研究科
• From Apr. 2019, To Mar. 2020

  Seminar on Biomolecular Information IIA

  Spring, 理学研究科
• From Apr. 2019, To Mar. 2020

  Seminar on Biomolecular Information IIB

  Fall, 理学研究科
• From Apr. 2019, To Mar. 2020

  Seminar on Biomolecular Information IIC

  Spring, 理学研究科
• From Apr. 2019, To Mar. 2020

  Seminar on Biomolecular Information IID

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Biomolecular Information IA

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Biomolecular Information IB

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Biomolecular Information IC

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Biomolecular Information ID

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Biomolecular Information IIA

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Biomolecular Information IIB

  Fall, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Biomolecular Information IIC

  Spring, 理学研究科
• From Apr. 2020, To Mar. 2021

  Seminar on Biomolecular Information IID

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Biomolecular Information IA

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Biomolecular Information IB

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Biomolecular Information IC

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Biomolecular Information ID

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Biomolecular Information IIA

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Biomolecular Information IIB

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Biomolecular Information IIC

  Spring, 理学研究科
• From Apr. 2021, To Mar. 2022

  Seminar on Biomolecular Information IID

  Fall, 理学研究科
• From Apr. 2021, To Mar. 2022

  Advanced Course on Biological Science B

  Fall, 理学研究科

Administration

Faculty management (title, position)

• From 01 Apr. 2023, To 31 Mar. 2024

  化学研究所放射線障害防止委員会
• From 01 Apr. 2017, To 31 Mar. 2019

  宇治地区化学物質管理委員会 委員
• From 01 Apr. 2017, To 31 Mar. 2018

  化学研究所総務・教務委員会 委員
• From 01 Apr. 2017, To 31 Mar. 2019

  化学物質専門委員会 委員
• From 01 Apr. 2020, To 31 Mar. 2021

  化学研究所広報委員会委員