Basic Information

Professional Memberships

• 「細胞を創る」研究会
• 日本RNA学会
• 日本分子生物学会

Academic Degree

• 修士（生命科学）(京都大学)
• 博士（生命科学）(京都大学)

ID,URL

• J-Global ID

  201901002352782147

researchmap URL

• https://researchmap.jp/f1m1-m2

Research

Research Interests

• protein
• 核酸工学
• RNA
• ケミカルバイオロジー
• ナノバイオテクノロジー

Research Areas

• Life sciences, Molecular biology

Papers

• Split RNA switch: a novel strategy for precise gene regulation using multiple RNA switches

  Itsuki Abe; Hirohisa Ohno; Hirohide Saito

  実験医学, Dec. 2025, Invited

  
• MDM4 enables efficient human iPS cell generation from PBMCs using synthetic RNAs

  Masato Nakagawa; Mizuho Nogi; Hatsuki Doi; Ryuhei Hayashi; Tomohiko Katayama; Hirohisa Ohno; Megumi Mochizuki; Karin Hayashi; Hirohide Saito

  Scientific Reports, 08 Sep. 2025, Peer-reviewed

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• Split RNA switch orchestrates pre- and post-translational control to enable cell type-specific gene expression

  Itsuki Abe; Hirohisa Ohno; Megumi Mochizuki; Karin Hayashi; Hirohide Saito

  Nature Communications, 01 Jul. 2025, Peer-reviewed, Corresponding author

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• MDM4 enables efficient human iPS cell generation from PBMCs using synthetic RNAs

  Masato Nakagawa; Mizuho Nogi; Hatsuki Doi; Hirohisa Ohno; Megumi Mochizuki; Karin Hayashi; Hirohide Saito

  16 Apr. 2025

  
• Synthetic mRNA: Diverse engineering strategies

  大野 博久; 齊藤 博英

  生化学, Dec. 2024, Invited, Lead author

  
• Split RNA switch: Programmable and precise control of gene expression by ensemble of pre- and post-translational regulation

  Itsuki Abe; Hirohisa Ohno; Megumi Mochizuki; Karin Hayashi; Hirohide Saito

  25 Sep. 2024

  
• Synthetic RNA–protein nanostructures and their potential applications

  Hirohisa Ohno; Eriko Osada; Tan Inoue; Hirohide Saito

  RNA Nanotechnology and Therapeutics, 01 Jan. 2013

  
• Programmable Computational RNA Droplets Assembled via Kissing-Loop Interaction

  Hirotake Udono; Minzhi Fan; Yoko Saito; Hirohisa Ohno; Shin-ichiro M. Nomura; Yoshihiro Shimizu; Hirohide Saito; Masahiro Takinoue

  ACS Nano, 03 Jun. 2024, Peer-reviewed

  
• Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules

  Shodai Komatsu; Hirohisa Ohno; Hirohide Saito

  Nature Communications, 17 Nov. 2023, Peer-reviewed

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• Versatile strategy using vaccinia virus-capping enzyme to synthesize functional 5′ cap-modified mRNAs

  Hirohisa Ohno; Sae Akamine; Megumi Mochizuki; Karin Hayashi; Shinichiro Akichika; Tsutomu Suzuki; Hirohide Saito

  Nucleic Acids Research, 03 Feb. 2023, Peer-reviewed

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• Synthetic circular RNA switches and circuits that control protein expression in mammalian cells

  Shigetoshi Kameda; Hirohisa Ohno; Hirohide Saito

  Nucleic Acids Research, 16 Jan. 2023, Peer-reviewed

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• Synthetic mRNA-Based Systems in Mammalian Cells.

  Hirohisa Ohno; Sae Akamine; Hirohide Saito

  Advanced biosystems, May 2020, Peer-reviewed

  
• RNA nanostructures and scaffolds for biotechnology applications.

  Hirohisa Ohno; Sae Akamine; Hirohide Saito

  Current opinion in biotechnology, Aug. 2019, Peer-reviewed

  
• Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate.

  Tomonori Shibata; Yoshihiko Fujita; Hirohisa Ohno; Yuki Suzuki; Karin Hayashi; Kaoru R Komatsu; Shunsuke Kawasaki; Kumi Hidaka; Shin Yonehara; Hiroshi Sugiyama; Masayuki Endo; Hirohide Saito

  Nature communications, 14 Sep. 2017, Peer-reviewed

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• RNA/RNP Nanotechnology for Biological Applications

  OHNO Hirohisa; SAITO Hirohide

  Seibutsu Butsuri, 2016, Peer-reviewed, Invited

  
• RNA and RNP as Building Blocks for Nanotechnology and Synthetic Biology.

  Hirohisa Ohno; Hirohide Saito

  Progress in molecular biology and translational science, 2016, Peer-reviewed

  
• Designed Regular Tetragon-Shaped RNA-Protein Complexes with Ribosomal Protein L1 for Bionanotechnology and Synthetic Biology.

  Hirohisa Ohno; Tan Inoue

  ACS nano, 26 May 2015, Peer-reviewed

  
• A purification method for a molecular complex in which a scaffold molecule is fully loaded with heterogeneous molecules.

  Shoji J Ohuchi; Fumihiko Sagawa; Hirohisa Ohno; Tan Inoue

  PloS one, 2015, Peer-reviewed

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• Design, assembly, and evaluation of RNA-protein nanostructures.

  Hirohisa Ohno; Eriko Osada; Hirohide Saito

  Methods in molecular biology (Clifton, N.J.), 2015, Peer-reviewed

  
• Cell-surface receptor control that depends on the size of a synthetic equilateral-triangular RNA-protein complex.

  Yoshihiko Fujita; Rie Furushima; Hirohisa Ohno; Fumihiko Sagawa; Tan Inoue

  Scientific reports, 19 Sep. 2014, Peer-reviewed

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• Engineering RNA-protein complexes with different shapes for imaging and therapeutic applications.

  Eriko Osada; Yuki Suzuki; Kumi Hidaka; Hirohisa Ohno; Hiroshi Sugiyama; Masayuki Endo; Hirohide Saito

  ACS nano, 26 Aug. 2014, Peer-reviewed

  
• Synthetic RNA-protein complex shaped like an equilateral triangle.

  Hirohisa Ohno; Tetsuhiro Kobayashi; Rinko Kabata; Kei Endo; Takuma Iwasa; Shige H Yoshimura; Kunio Takeyasu; Tan Inoue; Hirohide Saito

  Nature nanotechnology, Feb. 2011, Peer-reviewed

  

Misc.

• Abstract 4340954: Self-Amplifying RNA Enhances Cardiac Protein Expression and Functional Recovery in a Myocardial Infarction-Induced Heart Failure Murine Model

  Kazuma Handa; Hirohisa Ohno; Lisa Fujimura; Akima Harada; Tadashi Watabe; Julia Whitehouse; Kenji Miki; Hirohide Saitou; Shigeru Miyagawa

  Circulation, 04 Nov. 2025

  
• Relationship between the splicing pattern of the Drosophila sodium channel gene and the phenotypic variety

  Y. Tanaka; T. Koma; C. Hatai; R. Suzuki; H. Ohno

  International Congress Series, Nov. 2005, Peer-reviewed

  
• 3S4a06 RNP synthetic biology

  Saito Hirohide; Fujita Yoshihiko; Kashida Shunichi; Ohno Hirohisa; Endo Kei; Inoue Tan

  日本生物工学会大会講演要旨集, 2011

  

Books and Other Publications

• Molecular Robotics: An Introduction

  Contributor, "4.8 RNA Nanotechnology" (pp. 157-167), "7.1 Cell-Fate Control by RNA/RNP" (pp. 249-258)

  Springer, 18 Aug. 2022

  Find Kyoto University's collection of books :

  
• 遺伝学の百科事典 : 継承と多様性の源 = Encyclopedia of genetics : origin of inheritance and diversity

  日本遺伝学会, Contributor, 『3-20 翻訳, リボソーム』(pp.122-123)

  丸善出版, Jan. 2022

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• 相分離生物学の全貌（現代化学増刊46)

  白木賢太郎, Contributor, 『54. 生命の起源と相分離』pp.268-273

  東京化学同人, Nov. 2020

  
• 人類はなぜ宇宙へ行くのか (シリーズ〈宇宙総合学〉 3)

  土山明; 大野博久; 齊藤博英; 水村好貴; 大塚敏之; 山敷庸亮; 呉羽真; 大野照文; 京都大学宇宙総合学研究ユニット, Contributor, 『chapter 2 生命の起源と宇宙』pp. 26-58

  朝倉書店, Dec. 2019

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• 分子ロボティクス概論 : 分子のデザインでシステムをつくる

  分子ロボティクス研究会; 村田智, Contributor, 『3.8 RNAナノテクノロジーの動向（大野博久, 齊藤博英）』pp.163-180;『6.1 RNA/RNPによる細胞運命の制御（大野博久, 齊藤博英）』pp.247-263

  CBI学会, May 2019

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• RNA nanotechnology and therapeutics

  Guo, Peixuan; Haque, Farzin, Contributor, Hirohisa Ohno, Eriko Osada, Tan Inoue, Hirohide Saito, "Synthetic RNA–Protein Nanostructures and Their Potential Applications", pp.303-312

  CRC Press, Aug. 2013

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External funds: Kakenhi

• Development of a highly functional artificial mRNA platform for the realization of mRNA medicine

  Grant-in-Aid for Scientific Research (C)

  Basic Section 90120:Biomaterials-related

  Kyoto University

  Hirohisa Ohno

  From 01 Apr. 2020, To 31 Mar. 2024, Project Closed

  mRNA;遺伝子治療;キャップ;人工mRNA;5'キャップ;化学修飾;RNAワクチン;5' キャップ

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K12644/
• AutoMatter: Toward creation and expansion of programmable micro-active matter

  Grant-in-Aid for Scientific Research (S)

  Broad Section J

  Tohoku University

  Shin-ichiro Nomura

  From 31 Aug. 2020, To 31 Mar. 2025, Project Closed

  オートマター;人工細胞;自己複製;大量生産;電子-分子インタフェイス

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H05701/
• Creation and development of programmable dynamic microparticles "auto-matter"

  Grant-in-Aid for Scientific Research (A)

  Medium-sized Section 61:Human informatics and related fields

  Tohoku University

  野村 慎一郎

  From 01 Apr. 2020, To 31 Mar. 2021, Discontinued

  人工多細胞;人工細胞;分子ロボティクス;オートマター;自己複製;大量生産;電子-分子インタフェイス

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20H00598/
• Spatial control of intracellular molecules by the RNA-protein nanostructure

  Grant-in-Aid for Young Scientists (B)

  Kyoto University

  Hirohisa Ohno

  From 01 Apr. 2016, To 31 Mar. 2019, Project Closed

  RNA;RNA-タンパク質相互作用;分子足場;細胞運命制御;ナノ構造体;分子設計;細胞内局在;RNA-タンパク質複合体;ナノバイオ;バイオテクノロジー;核酸;蛋白質

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16K21117/
• RNA分子の集合によるリボザイム活性発現モデルの構築

  Grant-in-Aid for Challenging Research (Exploratory)

  Medium-sized Section 17:Earth and planetary science and related fields

  Kyoto University

  大野 博久

  From 30 Jun. 2023, To 31 Mar. 2026, Granted

  RNA;ヌクレオチド;リボザイム;RNAワールド

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23K17704/
• 非天然型の構造を有する高機能RNAベクターの開発

  Grant-in-Aid for Scientific Research (B)

  Basic Section 90120:Biomaterials-related

  Kyoto University

  大野 博久

  From 01 Apr. 2025, To 31 Mar. 2029, Granted

  人工mRNA;遺伝子治療;化学修飾

  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25K03464/

Academic, Social Contribution

Social Contribution

• 生命の起源と宇宙の接点～生命は宇宙から降ってきた？

  Lecturer

  お寺で宇宙学実行委員会, 第七回 お寺で宇宙学, From 03 Sep. 2011, To 03 Sep. 2011

  
• Science Codex No. 22, 宇宙に隣人を探して

  Contributor

  サイエンスカフェ・ガリレオ・ガリレイ, From Mar. 2011, To Mar. 2011

  
• Science Codex No. 4, 生命のもとは宇宙にあり？

  Contributor

  サイエンスカフェ・ガリレオ・ガリレイ, From Sep. 2009, To Sep. 2009