Quantification of Cyclin-CDK dissociation constants in living cells using fluorescence cross-correlation spectroscopy with green and near-infrared fluorescent proteins.
A novel red fluorescence dopamine biosensor selectively detects dopamine in the presence of norepinephrine in vitro.
Chihiro Nakamoto; Yuhei Goto; Yoko Tomizawa; Yuko Fukata; Masaki Fukata; Kasper Harpsøe; David E Gloriam; Kazuhiro Aoki; Tomonori Takeuchi
Molecular brain, 2021年12月06日, 査読有り, 責任著者
20 years of Developmental Cell: Looking forward.
Takashi Hiiragi; Amy S Gladfelter; Irene Miguel-Aliaga; Hilary A Coller; On Sun Lau; Heidi M McBride; Kazuhiro Aoki; Andreas Linkermann; Stefano Santaguida; Geoffrey Wasteneys; Nicolas C Rivron; Marta N Shahbazi; Miki Ebisuya; Madeline A Lancaster
Developmental cell, 2021年12月06日
Desiccation-induced fibrous condensation of CAHS protein from an anhydrobiotic tardigrade.
Synergistic antitumor effects of combination PI3K/mTOR and MEK inhibition (SAR245409 and pimasertib) in mucinous ovarian carcinoma cells by fluorescence resonance energy transfer imaging
Visualization of intracellular signaling with fluorescence resonance energy transfer-based biosensors
Aoki, K.
Protein Modifications in Pathogenic Dysregulation of Signaling, 2015年
Quantitative In Vivo Fluorescence Cross-Correlation Analyses Highlight the Importance of Competitive Effects in the Regulation of Protein-Protein Interactions
Fluorescence resonance energy transfer based quantitative analysis of feedforward and feedback loops in epidermal growth factor receptor signaling and the sensitivity to molecular targeting drugs
Fluorescence resonance energy transfer imaging of cell signaling from in vitro to in vivo: Basis of biosensor construction, live imaging, and image processing
Kazuhiro Aoki; Yuji Kamioka; Michiyuki Matsuda
Development Growth and Differentiation, 2013年05月, 査読有り, 責任著者
A Quantitative Model of ERK MAP Kinase Phosphorylation in Crowded Media
FRET imaging and statistical signal processing reveal positive and negative feedback loops regulating the morphology of randomly migrating HT-1080 cells
SH3BP1, an Exocyst-Associated RhoGAP, Inactivates Rac1 at the Front to Drive Cell Motility
Maria Carla Parrini; Amel Sadou-Dubourgnoux; Kazuhiro Aoki; Katsuyuki Kunida; Marco Biondini; Anastassia Hatzoglou; Patrick Poullet; Etienne Formstecher; Charles Yeaman; Michiyuki Matsuda; Carine Rosse; Jacques Camonis
Spatiotemporal regulation of small GTPases as revealed by probes based on the principle of f̈orster resonance energy transfer (FRET): Implications for signaling and pharmacology
E. Kiyokawa; K. Aoki; T. Nakamura; M. Matsuda
Annual Review of Pharmacology and Toxicology, 2011年, 査読有り
Revolving movement of a dynamic cluster of actin filaments during mitosis
Dynamics of the Ras/ERK MAPK cascade as monitored by fluorescent probes
A Fujioka; K Terai; RE Itoh; K Aoki; T Nakamura; S Kuroda; E Nishida; M Matsuda
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006年03月, 査読有り
Monitoring spatio-temporal regulation of Ras and Rho GTPases with GFP-based FRET probes
T Nakamura; K Aoki; M Matsuda
METHODS, 2005年10月, 査読有り
FRET imaging in nerve growth cones reveals a high level of RhoA activity within the peripheral domain
T Nakamura; K Aoki; M Matsuda
MOLECULAR BRAIN RESEARCH, 2005年10月, 査読有り
Mechanism and role of localized activation of Rho-family GTPases in growth factor-stimulated fibroblasts and neuronal cells
K Kurokawa; T Nakamura; K Aoki; M Matsuda
BIOCHEMICAL SOCIETY TRANSACTIONS, 2005年08月, 査読有り
Local phosphatidylinositol 3,4,5-trisphosphate accumulation recruits Vav2 and Vav3 to activate Rac1/Cdc42 and initiate neurite outgrowth in nerve growth factor-stimulated PC12 cells
K Aoki; T Nakamura; K Fujikawa; M Matsuda
MOLECULAR BIOLOGY OF THE CELL, 2005年05月, 査読有り
Improvement of the bioluminescence reporter system for real-time monitoring of circadian rhythms in the cyanobacterium Synechocystis sp strain PCC 6803
K Kucho; K Aoki; S Itoh; M Ishiura
GENES & GENETIC SYSTEMS, 2005年02月, 査読有り
Dynamics of rho-family G proteins as visualized with FRET-based probes
Y. Hisayoshi; A. Kazuhiro; M. Naoki; M. Matsuda
Folia Pharmacologica Japonica, 2005年, 査読有り
Spatio-temporal regulation of Rac1 and Cdc42 activity during nerve growth factor-induced neurite outgrowth in PC12 cells
光学 = Japanese journal of optics : publication of the Optical Society of Japan, 2020年01月
ERK MAPキナーゼによる細胞増殖と細胞集団運動の制御
青木一洋; 青木一洋
生化学, 2019年02月25日
赤色光/遠赤色光による哺乳類培養細胞のシグナル伝達系の光操作
後藤 祐平; 青木 一洋
生物物理, 2019年
Optogenetic Tools for Quantitative Biology: The Genetically Encoded PhyB–PIF Light-inducible Dimerization System and Its Application for Controlling Signal Transduction
Improvement of the biolumineseence reporter system for real-time monitoring of circadian rhythms in the cyanobacterium Synechocystis sp. strain PCC 6803 (vol 80, pg 19, 2005)
K Kucho; K Aoki; S Itoh; M Ishiura
GENES & GENETIC SYSTEMS, 2005年06月
Ras/ERK MAPKカスケードのダイナミクス
藤岡亜紀; 寺井健太; 伊藤玲奈; 青木一洋; 中村岳史; 黒田真也; 西田栄介; 松田道行
日本分子生物学会年会講演要旨集, 2005年
Local accumulation of PIP3 at plasma membrane recruits Vav2/Vav3 to activate Rac1/Cdc42 and to initiate outgrowth in NGF-stimulated PC12 cells
K Aoki; T Nakamura; M Matsuda
MOLECULAR BIOLOGY OF THE CELL, 2004年11月
講演・口頭発表等
Cytoplasmic fluidization triggers dormancy breaking in fission yeast spores
Kazuhiro Aoki
RIKEN BDR Symposium 2025 Towards Redesigning Lifecycles, 2025年03月05日, 招待有り
蛍光イメージングと光遺伝学による 細胞周期の定量解析
青木 一洋
大阪大学・ニコンイメージングセンターシリーズセミナー第27回, 2025年01月10日, 招待有り
ZO-1 shuttles between tight junctions and podosomes by riding ERK activation waves during collective cell migration
Kazuhiro Aoki
Kyoto University International Symposium“Dynamics in Cell Communications, 2024年12月04日, 招待有り
Toward understanding the physical characteristics of cells and their significance
Kazuhiro Aoki
MathHuB 2024 / Mathematical Human Biology of Shape and Movement, 2024年11月20日, 招待有り
Quantitative analysis of cell surface tension and cytoplasmic fluidity in cytokinesis and dormancy by live-cell imaging.
Kazuhiro Aoki
The 52nd Naito Conference on Frontiers of Physical and Mechanical Biology, 2024年10月02日, 招待有り
Imaging cell state transition of vegetative and spores in fission yeast.
Cytoplasmic fluidization triggers breaking spore dormancy in fission yeast
Kazuhiro Aoki
Mechanical Control of Biological Self-organization, 2024年06月17日
蛍光イメージングによる細胞周期の定量解析
青木一洋
「集え、多分野研究者!」 感染症キャンプin 宮崎, 2024年01月27日, 招待有り
Quantitative analysis of mechanical properties and their biological functions using live cell imaging and optogenetics
Kazuhiro AOKI
INFORMATION PHYSICS IN LIVING SYSTEMS, 2024年01月09日, 招待有り
Live cell imaging of cell signaling and cell fate decision
Kazuhiro AOKI
Exploratory Research Center on Life and Living Systems (ExCELLS) and KAIST Sympoisum on Complex Biological Systems (Japan-Korea Collaborative Symposium), 2023年11月24日
ExCELLS visit talks at Institute of Biological Chemistry, Academia Sinica, 2019年06月21日, 招待有り
Live-cell quantification of the concentration and dissociation constant of endogenous proteins by a combination of CRISPR/Cas9 genome editing and FCS/FCCS.
Kazuhiro Aoki
Interface between Immunology & Quantitative Biology, 2019年05月20日, 招待有り
ERK活性の細胞間伝搬による細胞集団運動の制御
青木一洋
細胞外環境と老化研究のフロンティア, 2019年03月02日, 招待有り
Optical Control of Cell Signaling by the Fully-Genetically Encoded PhyB-PIF System in Human Cells, Yeast and Worms